Ukufunyanwa kwe-mRNA ye-HR-HPV yohlobo lwe-15-Type – Kuchonga ubukho kunye nomsebenzi we-HR-HPV

Umhlaza womlomo wesibeleko, oyena nobangela uphambili wokufa phakathi kwabasetyhini kwihlabathi liphela, ubangelwa kakhulu lusulelo lwe-HPV. Amandla omhlaza osulelo lwe-HR-HPV axhomekeke ekubonakalisweni okwandileyo kwezakhi zofuzo ze-E6 kunye ne-E7. Iiproteni ze-E6 kunye ne-E7 zibophelela kwiiproteni zokunciphisa ithumba i-p53 kunye ne-pRb ngokulandelelana, kwaye ziqhuba ukwanda kunye nokuguqulwa kweeseli zomlomo wesibeleko.

Nangona kunjalo, uvavanyo lwe-HPV DNA luqinisekisa ukubakho kwentsholongwane, alubonisi phakathi kosulelo olufihlakeleyo noluguqulelwa ngokukuko. Ngokwahlukileyo koko, ukufunyanwa kwee-HPV E6/E7 mRNA transcripts kusebenza njenge-biomarker ecacileyo yokubonakaliswa kwe-viral oncogene esebenzayo, kwaye ke ngoko, luphawu oluchanekileyo lwe-cervical intraepithelial neoplasia (CIN) okanye i-invasive carcinoma.

I-HPV E6/E7 mRNAUvavanyo lubonelela ngeenzuzo ezibalulekileyo ekuthinteleni umhlaza womlomo wesibeleko:

• Uvavanyo oluchanekileyo loMngcipheko: Luchonga usulelo lwe-HPV olusebenzayo nolunobungozi obuphezulu, lubonelela ngovavanyo lomngcipheko oluchanekileyo kunovavanyo lwe-HPV DNA.
• I-Triage Esebenzayo: Ikhokela oogqirha ekuchongeni izigulana ezifuna uphando olongezelelekileyo, inciphisa iinkqubo ezingafunekiyo.
• Isixhobo Sokuhlola Esinokubakho: Singasebenza njengesixhobo sokuhlola esizimeleyo kwixesha elizayo, ingakumbi kubantu abasengozini enkulu.
• Iintlobo ezili-15 ze-High-risk Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR) evela kwi-#MMT, efumanisa ngokwemigangatho uphawu losulelo olunokwenzeka oluqhubekekayo lwe-HR-HPV, sisixhobo esiluncedo sokuhlola i-HPV kunye/okanye ulawulo lwezigulane.

Iimpawu zemveliso:

• Ugutyungelwe ngokupheleleyo: Iintlobo ezili-15 ze-HR-HPV ezinxulumene nomhlaza womlomo wesibeleko zigutyungelwe;
• Uvakalelo olugqwesileyo: iikopi ezingama-500/mL;
• Ukuchaneka okugqwesileyoAkukho msebenzi udibeneyo ne-cytomegalovirus, i-HSV II kunye ne-DNA ye-genomic yomntu;
• Ixabiso eliphantsi: Iinjongo zovavanyo zinxulumene kakhulu nesifo esinokubakho, ukunciphisa uvavanyo olungeyomfuneko ngeendleko ezongezelelweyo;
• Ukuchaneka okugqwesileyo: IC kuyo yonke inkqubo;
• Ukuhambelana okubanzi: Ngeenkqubo ze-PCR eziqhelekileyo;