Iintsholongwane zokuphefumla zeentlobo ezingama-29- Ukufunyanwa okukodwa kovavanyo olukhawulezileyo noluchanekileyo kunye nokuchongwa

Iintsholongwane ezahlukeneyo zokuphefumla ezifana nomkhuhlane, i-mycoplasma, i-RSV, i-adenovirus kunye ne-Covid-19 ziye zanda ngexesha elinye kule busika, zisongela abantu abasengozini, kwaye zibangela ukuphazamiseka kubomi bemihla ngemihla. Ukuchongwa ngokukhawuleza nangokuchanekileyo kweentsholongwane ezisulelayo kwenza ukuba izigulane zifumane unyango lwe-etiological kwaye kubonelela ngolwazi malunga namaqhinga okuthintela usulelo kunye nokulawula amaziko ezempilo karhulumente.

Uvavanyo lweMacro kunye neMicro (MMT) luqalise iMultiplex Respiratory Pathogens Detection Panel, ejolise ekuboneleleni ngesisombululo sokufumanisa ngokukhawuleza nangokusebenzayo sokuhlola nokubhala ukuze kufunyanwe, kujongwe kwaye kuthintelwe iintsholongwane zokuphefumla kwiikliniki nakwimpilo yoluntu.

Isisombululo soHlolo esijolise kwiintsholongwane ezili-14 zokuphefumla

I-Covid-19, umkhuhlane A, umkhuhlane B, i-adenovirus, i-RSV, intsholongwane ye-parainfluenza, i-metapneumovirus yabantu, i-rhinovirus, i-coronavirus, i-bocavirus, i-enterovirus, i-mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Streptococcus pneumoniae.

Isisombululo soHlolo lweePathogens zokuPhefumla ezili-14

Isisombululo sokuchwetheza esijolise kwiintsholongwane ezili-15 eziphezulu zokuphefumla

Umkhuhlane A H1N1 (2009), H1, H3, H5, H7, H9, H10; Umkhuhlane B BV, BY; ICoronavirus 229E, OC43, NL63, HKU1, SARS, MERS.

Isisombululo sokuchwetheza kwiintsholongwane zokuphefumla ezili-15

Isisombululo sokuHlola kunye nesisombululo sokuTypelisa singasetyenziswa ngokudibeneyo okanye ngokwahlukeneyo, kwaye ziyahambelana nezixhobo zokuhlola ezivela kubalingani ukuze zisetyenziswe ngokudibeneyo ngokuguquguqukayo kubathengi.' iimfuno.

Izisombululo zoHlolo kunye nokuTyhila ezinceda ekuxilongweni kwangethuba kunye nokujonga ubhubhane wezifo zomchamo ziya kuqinisekisa unyango oluchanekileyo kunye nokuthintela ukusasazeka kwezifo ngobuninzi.

Inkqubo yoVavanyo kunye neempawu zeMveliso

Ukhetho 1: NgeI-Eudemon™AIO800(Inkqubo yokukhulisa iimolekyuli ngokuzenzekelayo ngokupheleleyo) yaphuhliswa ngokuzimeleyo yi-MMT

Iingenelo:

1) Ukusebenza okulula: Isampuli ingenile kwaye iphume. Yongeza kuphela iisampulu zeklinikhi eziqokelelweyo ngesandla kwaye yonke inkqubo yovavanyo iya kugqitywa ngokuzenzekelayo yiNkqubo;

2) Ukusebenza kakuhle: Ukucubungula isampuli okudibeneyo kunye nenkqubo yokusabela ngokukhawuleza kwe-RT-PCR kwenza ukuba yonke inkqubo yovavanyo igqitywe kwisithuba seyure e-1, nto leyo eyenza kube lula unyango ngexesha elifanelekileyo kunye nokunciphisa umngcipheko wokudluliselwa kwesifo;

3) Uqoqosho: Itekhnoloji ye-Multiplex PCR + itekhnoloji yokuxuba i-reagent master inciphisa iindleko kwaye iphucula ukusetyenziswa kwesampulu, okwenza kube lula ukuyisebenzisa xa kuthelekiswa nezisombululo ze-POCT ezifanayo zemolekyuli;

4) Uvakalelo Oluphezulu kunye Nokuchaneka: I-LoD ephindaphindiweyo ukuya kuthi ga kwiikopi ezingama-200/mL kunye nokuchaneka okuphezulu kuqinisekisa ukuchaneka kovavanyo kwaye kunciphisa ukuxilongwa okungachanekanga okanye ukuxilongwa okungachanekanga.

5) Ukugubungela ngokubanzi: Iintsholongwane eziqhelekileyo zesifo sokuphefumla esibukhali ezigutyungelweyo, ezibalelwa kwi-95% yeentsholongwane kwiimeko eziqhelekileyo zosulelo lokuphefumla olubukhali ngokwezifundo zangaphambili.

Ukhetho 2: Isisombululo seMolekyuli esiQhelekileyo

Iingenelo:

1) Ukuhambelana: Ihambelana kakhulu nezixhobo ze-PCR eziphambili ezikwimarike;

2) Ukusebenza kakuhle: Inkqubo yonke igqityiwe kwiyure e-1, nto leyo eyenza kube lula unyango ngexesha elifanelekileyo kwaye kuncitshiswe umngcipheko wokosuleleka;

3) Uvakalelo Oluphezulu kunye Nokuchaneka: I-LoD ephindaphindiweyo ukuya kuthi ga kwiikopi ezingama-200/mL kunye nokuchaneka okuphezulu kuqinisekisa ukuchaneka kovavanyo kwaye kunciphisa ukuxilongwa okungachanekanga okanye ukuxilongwa okungachanekanga.

4) Ukugubungela ngokubanzi: Iintsholongwane eziqhelekileyo zeklinikhi ezigutyungelweyo, ezithatha i-95% yeentsholongwane kwiimeko eziqhelekileyo zosulelo lwemiphunga olugutyungelweyo ngokwezifundo zangaphambili.

5) Ukuguquguquka: Isisombululo sokuhlola kunye nesisombululo sokuthayipha singasetyenziswa ngokudibeneyo okanye ngokwahlukeneyo, kwaye sikwahambelana nezixhobo zokuhlola ezivela kubavelisi abafanayo ukuze zisetyenziswe ngokudibeneyo ngokuguquguqukayo kwiimfuno zabathengi.

Pulwazi lwemveliso

Ikhowudi yeMveliso	Igama lemveliso	Iintlobo zeSampulu
I-HWTS-RT159A	Iintlobo ezili-14 zeKit yokuFumanisa iiPathogens zokuPhefumla eziDibeneyo (iFluorescence PCR)	I-Oropharyngeal/ i-nasopharyngeal swab
I-HWTS-RT160A	Iintlobo ezingama-29 zeKit yokuFumanisa iiPathogens zokuPhefumla eziDibeneyo (iFluorescence PCR)