I-EasyAmp yi-Macro kunye noVavanyo oluNcinci—-Isixhobo esiphathwayo sokukhulisa i-Isothermal Fluorescence Isixhobo esihambelana neLAMP/RPA/NASBA/HDA

Ukusebenza okugqwesileyo kunye neSicelo esiBanzi

Easy Amp, ngeteknoloji yesothermal nucleic acid amplificationibonakaliswe ngokuphezuluubuntununtunu kunyemfutshaneukusabelaixesha ngaphandle kweemfuno ze inkqubo yokutshintsha ubushushu. Ngoko ke, ibevelaednjengeuninziitekhnoloji ethandwayo yovavanyo olukhawulezayo lwe-nucleic acid.Ngaphandle kobunzima tIinkqubo zokukhwela ibhayisekile, isixhobo esilula nesiphathwayo sokukhulisa i-isothermalinokwanelisa iyonke inkqubo yokusabela.

IMacro & Micro-Test iphuhlise ngokuzimeleyokwaye yasungulwai EasyAmp,isixhobo esiphathwayo se-fluorescent isothermal amplificationkwizicelo ezahlukeneyo.

Okwangoku, iEasy Amp iqinisekisiwe ukubaiyahambelana neendlela ezininzi zokukhulisa i-isothermal: I-Loop-Mediated Isothermal Amplification(LAMP), i-Recombinase Polymerase Amplification (RPA/RAA/MIRA), i-Nucleic Acid Sequence Based Amplification(NASBA), kunye ne-Helicase Dependent Amplification(HDA).Iya kuba sesinye isixhobo sokusika sovavanyo olukhawulezayo lwe-nucleic acid kunye nezicelo ezibanzi kakhulu.

· I-Loop-Mediated Isothermal Amplification (LAMP):Ukwandisa kwi60-65°Cngokupheleleyoukusabelainkquboigqitywe kwimizuzu engama-30.Ukubona uMfanekiso 1 wokukhulisaumgaqo:

 

Umzobo 1. Umgaqoof ISIbane 

· Hlanganisa kwakhona iPolymerase Amplification (RPA/RAA/MIRA):Ukwandisa kwi37°C-42°Cngokupheleleyoukusabelainkquboigqitywe kwimizuzu eyi-10-30.Ukubona uMfanekiso 2 wayoumgaqo wokukhulisa:

Umzobo 2. Umgaqoof Phinda udibanise iPolymerase Amplification

·I-Nucleic Acid Based Based Amplification (NASBA):Ukwandisa kwi42°Cngokupheleleyoukusabelainkquboigqitywe kwimizuzu engama-40-120.Ukubona uMfanekiso 3 wayoumgaqo wokukhulisa:

Umzobo 3. Umgaqoof I-Nucleic Acid eSequence Based Amplification

· Ukwandiswa koXhomekeke kwiHelicase (HDA):Ukwandisa kwi65°Cngokupheleleyoukusabelainkquboigqitywe kwi-70-90 imizuzu.Ukubona uMfanekiso 4 wayoumgaqo wokukhulisa:

Fig4.Umgaqoof Ukwandiswa kwe-Helicase eXhomekekileyo

· I EasyAmpyenzaukukhulisa okusebenzayo kunye nozinzo kubhaqo lwe-DNA kunye ne-RNA kusetyenziswa iindlela ezine ze-isothermal ezikhankanywe ngasentla.Ifezekisa ubuntununtunu bokukhulisa ukuya kuthi ga kwiikopi ze-200 / mL kwaye iqinisekisa ukuchaneka okuphezulu ngaphandle kokusebenza kwakhona kwithagethi yokukhulisa..(Iiboniswe kwiMifanekiso 5 ukuya ku-7).

Ukufunyanwa kwe-DNA ye-RNA

Isazobe sesi-5.I-RAA/MIRA Platform

Ukufunyanwa kwe-DNA ye-RNA

Isazobe 6.Iqonga lesibane

 

Ukufunyanwa kwe-DNA ye-RNA

Isazobe 7.Iqonga leNASBA

Izinto eziluncedo kwiMveliso:

Ngokukhawuleza

· EPIAiteknoloji iyaqondaisiphumo esihleifunyenwe kwi-5 min.

· Ukusebenza ngokulula kunye nokutsalwa kwenyathelo elinyeukudibanisa kwisixhobo.

Esebenzayo

· Iimodyuli ezi-4 ezizimeleyo zokufunwa, uvavanyo ngaxeshanye lwe-4 ezahlukeneyouhlobo lokuvavanyas.

· Imodyuli nganye ekwaziyo ukuhlalutya iisampuli ezine ngaxeshanye.

· Zimbini/Inefluorescenceimijeloukubhaqa okujoliswe kuko okuninzi.

Ukuhambelana

· Iyahambelana neezininziiiplatifti ze-isothermal ezifana ne-LAMP, i-RPA, i-NASBA, i-HDA.

· Yenzelwe ukukhulisa ubushushu obuphezulu emva koshicilelo olubuyela umva lobushushu obuphantsi.

· Customizuyakwaziukuhlangabezana neemfuno ezithile zabaxhasi.

Isebenziseka kakuhle

· Puyilo ortable(290×245×128mm, 2kg) ukuba izicelo ezahlukeneyo senarios.

· 7-intshi umbala touchscreenukusebenza lula kunye nomfanekiso ocacileyo weziphumo.

· Iprinta eyakhelwe-ngaphakathi ye-thermal yoshicilelo olukhawulezileyo lweziphumo.

· Iskena esidityanisiweyo sokufunda into ezenzekelayo.