I-Easdamp ngovavanyo lwe-macro kunye novavanyo oluphawulekayo-i-isothermal fluoresces ye-amlmal slowning ehambelana nesibane / iRPA / NASBA / HDA

Ukusebenza ngokusebenza kakuhle kunye nokusetyenziswa ngokubanzi

I-AMP elula, ngetekhnoloji ye-II-sothermal ye-nucleid ye-acidiboniswe phezuluimvakalelo kunyemfutshaneimpenduloixesha ngaphandle kweemfuno ze Iqondo lobushushu lenkqubo. Ke ngoko, ibeiveleednjengeuninziItekhnoloji ethandwayo yokuvavanywa ngokukhawuleza kwe-acid ngokukhawuleza.Ngaphandle kwe-tIinkqubo zebhayisikile ezihamba ngebhayisikile, isixhobo esilula nesiphathwayounokwanelisaInkqubo yokuphendula yonke.

I-Macro kunye neMicro-Micro-micro iphuhlile ngokuzimeleyoyasungulwaI-Easdamp,Isixhobo esiphathwayo seFluorescent ye-Isothermal ye-stathermalkwizicelo ezahlukeneyo.

Okwangoku,I-AMP elula iqinisekisiwe ukuba ibeIhambelana neendlela ze-isothermal ezahlukeneyo ze-isothermal ye-isothermal ye-isothermal (isibane), lungisa i-pulymmerase ye-pulymmerase (i-RPA / raa / mira), i-NASBA), kwaye i-HEACLEADER AMAZIKO YOKUFUNDA (HDA). Iya kuba yenye isixhobo esinqunywe ngokusika ukuvavanywa ngokukhawuleza kwe-acid enezicelo ezibanzi.

I-Solop-Acthermal ye-isothermal ye-isothermal (isibane):I-Amlphation kwiI-60-65 ° Ciyonkeimpenduloinkquboigqitywe ngaphakathi kwemizuzu engama-30.Ukubona umzobo 1 ngenxa ye-amelumgaqo:

 

Umzobo 1. Umgaqoof Isibane 

· I-revzincinase ye-Polymerase ye-Polymerase (RPA / raa / mira):I-Amlphation kwiI-37 ° C-42 ° Ciyonkeimpenduloinkquboigqityiwe kwimizuzu eli-10 ukuya kwengama-30.Ndibona umzobo 2 kuyoUmgaqo we-Ampha:

Umzobo 2. Umgaqoof Phinda ufumane i-Polymerase Amplication

·Ukulandelelana kwe-nucleidI-Amlphation kwiI-42 ° Ciyonkeimpenduloinkquboigqityiwe kwimizuzu engama-40-120.Ukubona umzobo 3 ngenxa yayoUmgaqo we-Ampha:

Umzobo 3. Umgaqoof Ukulandelelana kwe-nucleid i-acid

I-Amplieling ePhakamileyo (HDA):I-Amlphation kwiI-65 ° Ciyonkeimpenduloinkquboigqitywe kwimizuzu engama-70-90.Ukubona umzobo 4Umgaqo we-Ampha:

Umzobo Umgaqoof Ukutsala ukuxhomekeka

· · ToampyenzaI-amerition esebenzayo nenezinto ezizinzileyo kwi-DNA kunye ne-RNA yokuhlolwa kwe-RNA kusetyenziswa ezona ndlela zikhankanyiweyo ngaphambili. Ifezekisa imvakalelo yokuhlola i-Amlphaitiver ukuya kwi-200 yeekopi / i-ML kwaye iqinisekisa ubunyani obuphezulu ngaphandle kokuphinda-phinda kwethagethi.(Iifakwe kwi-5 ukuya kwi-7).

I-DNA yokufumana i-DNA

Umfanekiso Iplati / iqonga le-mira

I-DNA yokufumana i-DNA

Umzobo. Iqonga le-lamp

 

I-DNA yokufumana i-DNA

Umzobo Iqonga le-NASBA

Izibonelelo zeMveliso:

Ngokukhawuleza

· · EnesiaItekhnoloji iyayibonaIsiphumo esihleifunyenwe kwimizuzu emi-5.

Ukusebenza ngokusebenza ngenyathelo elinyeI-Ampling kwisixhobo.

Ngokufanelekileyo

Iimodyuli eziyi-4 ezizimeleyo ze-on-can, ekuvavanyeni kwalo ngaxeshanye ze-4 ezahlukeneyoUhlobo lovavanyos.

Imodyuli ye-modyuli ekwaziyo ukuhlalutya iisampulu ezine ngexesha ngalinye.

· · · · Diad / quadfluorescenceIindlelaUkufumana iqashiso eliphezulu.

Ukuhambelana

· · · AtyenzisileyoezininziAmaqonga eSothermal anje ngesibane, iRPA, Nasba, HDA.

Yenzelwe ukuba kubekho amaqondo obushushu obushushu obuphakathi kweqondo lokuhlawula amaqondo obushushu.

· · · · · · · · · · · · · ·FituyakwaziUkufezekisa iimfuno ezithile zabathengi.

Isebenziseka kakuhle

· P · puyilo olugcweleyo(290×245×1I-28mm, 2kg) ye I-Senarios ezahlukeneyo.

I-TourcesCreen ye-InchNgendlela elula kunye nesiphumo esicacileyo nesiphumo.

Umshicileli we-rimer of we-irmal owuprinta ngokukhawuleza.

Iskenari edibeneyo yento yokufunda ngokuzenzekelayo.