Uvavanyo lwe-EasyAmp ngeMacro kunye neMicro—-Isixhobo Sokukhulisa I-Isothermal Fluorescence Esiphathekayo Esihambelana neLAMP/RPA/NASBA/HDA

Ukusebenza Okugqwesileyo kunye nesicelo esibanzi

I-Easy Amp, ngetekhnoloji ye-iukwandiswa kwe-nucleic acid ye-sothermalibonakaliswa ngobuninziuvakalelo kunyemfutshaneimpenduloixesha elingenazo iimfuneko inkqubo yokutshintsha ubushushu. Ngoko ke, ibekuvelaednjengeuninziiteknoloji ethandwayo yokuvavanya ngokukhawuleza i-nucleic acid.Ngaphandle kobunzimaiinkqubo zokujikeleza iibhayisekile zezityalo, isixhobo sokukhulisa isothermal esilula nesiphathekayoinokwanelisayonke inkqubo yokusabela.

I-Macro kunye ne-Micro-Test ziye zaphuhliswa ngokuzimeleyokwaye yaqaliswai-EasyAmp,isixhobo sokukhulisa isothermal esiphathwayo esikhanyayokwizicelo ezahlukeneyo.

Okwangoku,I-Amp elula iqinisekisiwe ukubaiyahambelana neendlela ezahlukeneyo zokukhulisa i-isothermal: i-Loop-Mediated Isothermal Amplification (LAMP), i-Recombinase Polymerase Amplification (RPA/RAA/MIRA), i-Nucleic Acid Sequence Based Amplification (NASBA), kunye ne-Helicase Dependent Amplification (HDA)Iza kuba sesinye isixhobo esiphambili sokuvavanya ngokukhawuleza i-nucleic acid kunye nokusetyenziswa okubanzi kakhulu.

· Ukwandiswa kwe-Isothermal okuPhakathiswa yiLoop (ISIBANI):Ukwandiswa kwesandi kwi60-65°Ckunye nayo yonkeimpenduloinkquboigqityiwe kwimizuzu engama-30.Ukubona uMfanekiso 1 ukuze uphucule indlela osebenza ngayoumgaqo:

 

Umfanekiso 1. Umgaqoof ISIBANI 

· Ukwandiswa kwePolymerase yeRecombinase (RPA/RAA/MIRA):Ukwandiswa kwesandi kwi37°C-42°Ckunye nayo yonkeimpenduloinkquboigqityiwe kwimizuzu eli-10-30.Ukubona uMfanekiso 2umgaqo wokukhulisa:

Umfanekiso 2. Umgaqoof Ukwandiswa kwePolymerase yeRecombinase

·Ukwandiswa okuSekelwe kwiNucleic Acid Sequence (NASBA):Ukwandiswa kwesandi kwi42°Ckunye nayo yonkeimpenduloinkquboigqityiwe kwimizuzu engama-40-120.Ukubona uMfanekiso 3umgaqo wokukhulisa:

Umfanekiso 3. Umgaqoof Ukwandiswa okuSekelwe kwiNucleic Acid Sequence

· Ukwandiswa kweHelicase (HDA):Ukwandiswa kwesandi kwi65°Ckunye nayo yonkeimpenduloinkquboigqityiwe kwimizuzu engama-70-90.Ukubona uMfanekiso 4umgaqo wokukhulisa:

Umfanekiso 4. Umgaqoof Ukukhulisa okuxhomekeke kwiHelicase

· I-EasyAmpvumelaUkwandiswa okusebenzayo nokuzinzileyo kokufunyanwa kwe-DNA kunye ne-RNA kusetyenziswa iindlela ezine ze-isothermal ezikhankanyiweyo apha ngasentla. Ifikelela kulwandiso olufikelela kwiikopi ezingama-200/mL kwaye iqinisekisa ukucaciswa okuphezulu ngaphandle kokwenza izinto ngokudibeneyo ekukhuleni okujoliswe kuko..(Ikuboniswe kwiMifanekiso 5 ukuya ku-7).

Ukufunyanwa kwe-DNA Ukufunyanwa kwe-RNA

Umfanekiso 5. Iqonga le-RAA/MIRA

Ukufunyanwa kwe-DNA Ukufunyanwa kwe-RNA

Umfanekiso 6. Iqonga leSIBANI

 

Ukufunyanwa kwe-DNA Ukufunyanwa kwe-RNA

Umfanekiso 7. Iqonga le-NASBA

Iingenelo zeMveliso:

Ngokukhawuleza

· I-EPIAitekhnoloji iyafezekisaiziphumo ezilungileyoifunyenwe kwimizuzu emi-5.

· Ukusebenza okulula ngokukhupha inyathelo elinyeyokwandisa isixhobo.

Isebenza kakuhle

· Iimodyuli ezizimeleyo ezi-4 zovavanyo olufunekayo, oluqhutywa ngaxeshanye lwezi-4 ezahlukeneyouhlobo lovavanyos.

· Imodyuli nganye ekwaziyo ukuhlalutya iisampulu ezine ngaxeshanye.

· Isibini/Ikotafukukhanyaiitshaneliukufumanisa ngokubanzi izinto ezininzi ezijolise kuzo.

Ukuhambelana

· Iyahambelana neezininziamaqonga angabonakaliyo afana neLAMP, RPA, NASBA, HDA.

· Yenzelwe ukubhalwa okungasemva kobushushu obuphezulu emva kobushushu obuphantsi.

· Yenza ngokwezifisounakoukuhlangabezana neemfuno ezithile zabathengi.

Isebenziseka kakuhle

· Puyilo lwetafile elungiselelweyo(290×245×128mm, 2kg) ye ii-senarios ezahlukeneyo zesicelo.

· Isikrini sokuchukumisa esinombala we-intshi ezi-7ukuze kube lula ukuyisebenzisa kwaye kube nemifanekiso ecacileyo.

· Iprinta yobushushu eyakhelwe ngaphakathi ukuze ifumane iziphumo ezikhawulezileyo.

· Iskena esidibeneyo sokufunda izinto ngokuzenzekelayo.