Iqela B Streptococcus nucleic acid ukubona ikiti (Enzymatic Probe Isothermal Amplification)
1.Ukubaluleka kokufumanisa
Iqela B streptococcus (GBS) lidla ngokufakwa kwilungu lobufazi kunye nerectum yabasetyhini, nto leyo enokukhokelela kusulelo oluhlaselayo lwangethuba (GBS-EOS) kwiintsana ezisandul’ ukuzalwa ngokosulelo olusuka kumama lusiya emntwaneni, kwaye ngoyena nobangela wenyumoniya engekazalwa, imeningitis, i-septicemia kunye nokufa.Ngo-2021, iNgxowa-mali yeNgcali malunga noLawulo lweKlinikhi yoMngcipheko ophezulu waBasanda kuzalwa abaneSifo sokuQala kwiGumbi elifanayo loMama kunye neNtsana yaseTshayina yoMbutho weMpilo kaMama kunye noMntwana ucebise ukuba ukuhlolwa kwe-GBS kwiiveki ezingama-35-37 ngaphambi kokunikezelwa kunye nokuthintela i-intrapartum antibiotic (IAP). ) yayingamanyathelo asebenzayo okuthintela i-GBS-EOS kwiintsana ezisanda kuzalwa.
2. Imingeni ejongene neendlela zangoku zokubona
Ukuqhawuka kwangaphambi kwexesha kweembrane (PROM) kubhekiselele ekuqhawukeni kwenwebu phambi kokubeleka, okuyingxaki eqhelekileyo kwixesha lokubeleka.Ukugqabhuka kwangaphambi kwexesha kweembrane Ngenxa yokugqabhuka kweembrane, i-GBS kwilungu lobufazi labasetyhini ababandakanyekayo kunokwenzeka ukuba isasazeke ukuya phezulu, okukhokelela kusulelo lwe-intrauterine.Umngcipheko wosulelo uhambelana ngokuthe ngqo ekukhuleni ngexesha lokuqhekeka kweembrane (> 50% yabasetyhini abakhulelweyo bazala kwiiyure ezi-1-2h emva kokugqabhuka kweembrane, okanye kwiiyure ezingama-1-2).
Iindlela zokubona ezikhoyo azikwazi ukuhlangabezana ngokupheleleyo neemfuno zexesha (< 1h), ukuchaneka kunye nokufunyanwa kwe-GBS ekufowuneni ngexesha lokunikezelwa.
| Izixhobo zokufumanisa | Inkcubeko yebhaktheriya | Ixesha lenkcubeko : 18-24hUkuba uvavanyo lweziyobisi : ukwanda kwe-8-16h | 60 % izinga lokufunyanwa okulungileyo;Ngexesha lenkqubo yesampulu, ichaphazeleka kwiibhaktheriya ezinjenge-Enterococcus faecalis ejikeleze i-vagina kunye ne-anus, okukhokelela kwiziphumo ezingezizo ezingalunganga / ezingeyonyani. |
| Immunochromatography | Ixesha lokubona: 15min. | Uvakalelo luphantsi, kwaye kulula ukuphosakela ukubonwa, ngakumbi xa inani lebhaktheriya lincinci, kunzima ukulibona, kwaye izikhokelo zicetyiswa ngaphantsi. | |
| I-PCR | Ixesha lokubona : 2-3h | Ixesha lokufumanisa lingaphezu kweeyure ze-2, kwaye isixhobo se-PCR kufuneka sivavanywe kwiibhetshi, kwaye akunakwenzeka ukulandela uvavanyo. |
3. Macro & Micro-Test imveliso ebalaseleyo
Ukufunyaniswa ngokukhawuleza: Ukusebenzisa i-enzyme enelungelo elilodwa lomenzi wokucolisa inkqubo probe indlela yokukhulisa ubushushu obungaguqukiyo, izigulane ezine-positive zingasazi isiphumo ngokukhawuleza kwimizuzu emi-5.
Ukufumanisa nangaliphi na ixesha, akukho mfuneko yokulinda: ixhotywe ngeqondo lokushisa eliqhubekayo le-nucleic acid amplification analyzer Easy Amp, kwaye iimodyuli ezine ziqhuba ngokuzimeleyo, kwaye iisampuli zihlolwe njengoko zifika, ngoko akukho mfuneko yokulinda iisampuli.
Uhlobo oluninzi lweesampulu: i-vaginal swab, i-rectal swab okanye i-swab ye-vaginal exubileyo inokufunyanwa, ehlangabezana nezindululo zemigaqo ye-GBS, iphucula izinga lokufumanisa okulungileyo kunye nokunciphisa izinga lokuxilongwa kakubi.
Ukusebenza okugqwesileyo: iisampulu ezininzi zesampulu eqinisekisiweyo yeklinikhi (> iimeko ze-1000), ubuntununtunu 100%, ngokukodwa 100%.
Vula i-reagent: iyahambelana nesixhobo sePCR esiqhelekileyo se-fluorescence quantitative.
4. Ulwazi lwemveliso
| Inombolo yeMveliso | Igama lemveliso | Inkcazo | Inombolo yesatifikethi sobhaliso |
| HWTS-UR033C | Iqela B Streptococcus nucleic acid ukubona ikiti (I-Enzymatic Probe Isothermal Amplification) | Iimvavanyo ezingama-50/ikhithi | China Machinery Registration 20243400248 |
| HWTS-EQ008 | Easy Amp Real-time Fluorescence Isothermal Ukufunyanwa System | HWTS-1600P (4-itshaneli) | China Machinery Registration 20233222059 |
| HWTS-1600S (2-channel) | |||
| HWTS-EQ009 |
Ixesha lokuposa: Mar-07-2024