1. Ukubaluleka kokufumanisa
I-Group B streptococcus (GBS) idla ngokuhlala kwi-vagina yabasetyhini kunye ne-rectum, nto leyo enokubangela usulelo olungena ngokukhawuleza (GBS-EOS) kwiintsana ezisandul’ ukuzalwa ngokudluliselwa ngokuthe nkqo ukusuka kumama ukuya kumntwana, kwaye yeyona nto iphambili ebangela i-pneumonia yeentsana ezisandul’ ukuzalwa, i-meningitis, i-septicemia kwanokufa. Ngo-2021, i-Expert Consensus on Clinical Management of High-Risk Newborns with Early-onset Infection in the Same Room of Mothers and Infants of China Maternal and Child Health Association icebise ukuba ukuhlolwa kwe-GBS kwiiveki ezingama-35-37 ngaphambi kokubeleka kunye nokuthintela i-antibiotic ngaphakathi kwesisu (IAP) yayiziindlela ezisebenzayo zokuthintela i-GBS-EOS kwiintsana ezisandul’ ukuzalwa.
2. Imingeni ejongene neendlela zangoku zokufumanisa
Ukuqhekeka kwe-membrane ngaphambi kwexesha (i-PROM) kubhekisa ekuqhekekeni kwe-membrane ngaphambi kokuba kubeleke, nto leyo exhaphakileyo kwixesha lokuzala. Ukuqhekeka kwe-membrane ngaphambi kwexesha Ngenxa yokuqhekeka kwe-membrane, i-GBS kwisini sabasetyhini abakhulelweyo kunokwenzeka ukuba isasazeke iye phezulu, nto leyo ebangela usulelo lwangaphakathi kwesibeleko. Umngcipheko wosulelo uhambelana ngqo nokukhula ngexesha lokuqhekeka kwe-membrane (> 50% yabasetyhini abakhulelweyo bazala kwisithuba seeyure ezi-1-2 emva kokuqhekeka kwe-membrane, okanye kwaneeyure ezi-1-2).
Iindlela zokufumanisa ezikhoyo azikwazi ukuhlangabezana ngokupheleleyo neemfuno zokufaneleka ngexesha elifanelekileyo (< 1h), ukuchaneka kunye nokufunyanwa kwe-GBS ngexesha lokuhanjiswa.
| Izixhobo zokufumanisa | Inkcubeko yebhaktheriya | Ixesha lenkcubeko: 18-24hUkuba uvavanyo lobuthathaka beziyobisi: yonyusa iiyure eziyi-8-16 | Izinga lokufunyanwa kwentsholongwane eliyi-60%; ngexesha lenkqubo yokuthathwa kwesampuli, ichaphazeleka ziibhaktheriya ezifana ne-Enterococcus faecalis ezijikeleze i-vagina kunye ne-anus, nto leyo ekhokelela kwiziphumo ezingezizo ezilungileyo/ezingezizo ezilungileyo. |
| I-Immunochromatography | Ixesha lokufumanisa: 15min. | Uvakalelo luphantsi, kwaye kulula ukungayiboni, ingakumbi xa inani leebhaktheriya lincinci, kunzima ukulibona, kwaye izikhokelo azicetyiswa kangako. | |
| I-PCR | Ixesha lokufumanisa: iiyure ezi-2-3 | Ixesha lokufumanisa lingaphezulu kweeyure ezi-2, kwaye isixhobo se-PCR kufuneka sivavanywe ngokwamaqela, kwaye akunakwenzeka ukulandela uvavanyo. |
3. Iimpawu eziphambili zemveliso yeMacro kunye neMicro-Test
Ukufunyanwa ngokukhawuleza: Ukusebenzisa indlela yokukhulisa ubushushu obungaguqukiyo ye-enzyme digestion probe, izigulane ezine-HIV zingayazi isiphumo emva kwemizuzu emi-5.
Ukufunyanwa nangaliphi na ixesha, akukho mfuneko yokulinda: ixhotyiswe nge-nucleic acid amplification analyzer engaguqukiyo ye-Easy Amp, kwaye iimodyuli ezine zisebenza ngokuzimeleyo, kwaye iisampulu zihlolwa njengoko zifika, ngoko ke akukho mfuneko yokulinda iisampulu.
Uhlobo lweesampulu ezininzi: i-vaginal swab, i-rectal swab okanye i-mixed vaginal swab inokubonwa, ehambelana neengcebiso zezikhokelo ze-GBS, iphucula izinga lokufunyaniswa kakuhle kwaye inciphise izinga lokufunyaniswa okungafanelekanga.
Ukusebenza kakuhle kakhulu: ukuqinisekiswa kweklinikhi kwesampulu enkulu yeziko elininzi (> iimeko ezili-1000), uvakalelo lwe-100%, ukucaciswa kwe-100%.
I-reagent evulekileyo: iyahambelana nesixhobo se-PCR esisetyenziswa kakhulu kwi-fluorescence quantitative.
4. Ulwazi lwemveliso
| Inombolo yeMveliso | Igama lemveliso | Inkcazo | Inombolo yesatifikethi sobhaliso |
| I-HWTS-UR033C | Ikhithi yokufumanisa i-Streptococcus nucleic acid yeQela B (Ukwandiswa kwe-Enzymatic Probe Isothermal) | Uvavanyo lwe-50/ikhithi | Ubhaliso loomatshini baseTshayina 20243400248 |
| I-HWTS-EQ008 | Inkqubo yokuFumanisa i-Fluorescence Isothermal ye-Easy Amp Real-time | I-HWTS-1600P (iitshaneli ezi-4) | Ubhaliso loomatshini baseTshayina 20233222059 |
| I-HWTS-1600S (iitshaneli ezi-2) | |||
| I-HWTS-EQ009 |
Ixesha leposi: Matshi-07-2024