Isixhobo esitsha se-Tuberculosis sokolusi kunye nokuhlolwa kweziyobisi
Ingxelo yoncwadi: I-CCA: Imodeli yokuxilonga esekwe kwi-TNS kunye ne-tngs yokufunda, elungele abantu abanesifo sentsholongwane esincinci kunye ne-tebberculous meningitis.
Isihloko seTesis: I-Teberchgramics-Esolouch ejolise kuyo kunye nokufunda komatshini: Isicwangciso-qhinga sobuchule bokuxilonga se-UlCaifiki weTusifiki
Ixesha: "I-COSCICA Chimica Arta"
Ukuba: 6.5
Umhla woPapasho: NgoJanuwari 2024
Idityaniswe neDyunivesithi yaseTshayina yeSayensi kunye neSibhedlele saseBeijing School of Tentral University, iMacro neMicro-Micro-Micro-Paysion yaseTuebenesis ngokusekwe kwiTekhnoloji entsha yokuLawulwa kweKholeji kunye nendlela yokufunda ye-TNGS, ebonelela kakhulu Ukufunyanwa kwengqondo yesifo sephepha esinengxaki yentsholongwane kunye ne-meningitis ye-tubergous, inike indlela entsha ye-hypersitity yendlela yendlela yekliniki UKUQEQESHWA KWEZIQHELWENI ZESIFUNDO SE-TUBERculosis, kwaye wanceda ekucikisweni kwengqondo ngokuchanekileyo, ukunyangwa kweziyobisi kunye nonyango lwesifo sephepha. Kwangelo xesha, kufunyaniswe ukuba iPlasma yePlasma yesigulana inokusetyenziswa njengohlobo lwesampulu efanelekileyo yesampulu yekliniki ekufunyanisweni kwe-TB.
Kolu phando, iisampulu zeplasma ezingama-227 kunye neesampulu ze-cerbrospinal zasetyenziselwa ukuseka izihombo ezimbini zeklinikhi, apho iisampulu ze-Laboratric ezisetyenziselwa ukunyanzelwa kwe-tuberculosis, kunye neesampulu zeklinikhi zisetyenzisiwe ukuqinisekisa okusekwe Imodeli yokuqonda isifo. Zonke iisampuli zaqala ekujoliswe kuko kwi-Pobe ye-Pebege elungiselelwe ngokukodwa kwi-Mycobacterium Tuberculosis. Emva koko, ngokusekwe kwi-TB-TNGS yokulandelelana kwedatha, imodeli yomthi womthi isetyenziselwa ukwenza ukuqinisekiswa kwe-5-for proces uqeqesho kunye neeseti zokunyanzelwa kwelebhu yokugqibela, kunye nemilinganiselo yokuxilonga ye-Laboratoris kunye neesampulu ze-cerebrossinal zifunyenwe. Umbundu ofunyenwe ungeniswa kwiiseti zovavanyo ezimbini zokufumana iKliniki yeKlinikhi yokufunyanwa, kwaye umsebenzi wokusebenza komfundi uvavanywa nguRoc curve. Ekugqibeleni, kwafunyanwa imodeli yesifo sephepha.
Umzobo 1 we-schematic umzobo woyilo lophando
IZIPHUMO: Ngokwemilinganiselo ethile yesampulu ye-CSF ye-CSF (AUC = 0.974) isampulu yePlasma CFDNA Imvakalelo kunye nokuthintelwa kwesampulwa yeplasma yayingama-82.61% kunye ne-86.36%. Kuhlalutyo lweesampulu ezingama-44 ze-Plasma CFDNA kunye ne-DECEBROSINAL FLAID ezivela kwizigulana ze-tbm, isicwangciso-qhinga sokuxilonga sinokungqinelana kwePlasma CFDNA kunye nemvakalelo ye-95.45% (42/44). Kubantwana abanesifo sephepha sokuprinta, isicwangciso sokuxilongwa kolu phononongo sinovelwano ngakumbi kwiisampulu zePlasma kuneziphumo zokuchonga iisampulu ze-Xpert ezivela kwizigulana ezifanayo (ezingama-28.57% vs 15.38%).
Umzobo 2 Ukuvavanywa kokusebenza kwemodeli ye-tuberculo yesifo sephepha
Imiphumo emi-3 yokuxilongwa kweesampulu ezibini
Ukuqukumbela: Indlela yokuxilongwa nge-hypersostical ye-tuberculosis yasekwa kolu phononongo, enokubonelela ngesixhobo sokufumanisa okuphezulu kwezona zihlobo zibalulekileyo kwizigulana (inkcubeko engeyo). Ukufunyanwa kwe-tuberculotives ye-hypesculotives esekwe kwiPlasma CFDNA inokuba luhlobo olufanelekileyo lwesifo sephepha esisebenzayo se-tuberculous (iisampulu ze-tubergous (iisampulu zePlasma kulula ukuyiqokelela i-cerebrosclosis yesifo sephepha).
Ikhonkco loqobo: https/0W) nge-% 3dihub
Ngokwamfutshane ukuqaliswa kweMveliso yokuBonelelanisa iBeration Tuble
Xa ujonga imeko yesandla enzima yeziguli kunye neemfuno ezahlukeneyo, uvavanyo lwe-MACRO & NOR-Micro ubonelela ngeseti epheleleyo yezisombululo ze-NGS, ilayibrari yelayibrari ye-Sputum kunye nohlalutyo lwedatha. Iimveliso zigubungela ukufunyanwa kwesifo sephepha, ukunyangwa kwesifo sephepha, ukuchwetheza kwe-Mycobacterium Tuberculosis kunye ne-NTM, isifo se-NTERSINITISITION, isifo sephepha esinengxaki, njl.
Ukufunyanwa kwe-sertiation ye-tuts yesifo sephepha kunye ne-mycobacteria:
| Into | Igama lemveliso | Umxholo wovavanyo lwemveliso | Uhlobo lwesampulu | Imodeli esebenzayo |
| I-HWTS-3012 | Isampulu yokukhutshwa kwesampulu | Isetyenziswe kunyango olungalunganga lweesampulu zesikhohlela, ifumene inombolo yerekhodi yeklasi yokuqala, izixhobo zomatshini weSutong 20230047. | sputum | |
| I-HWTS-NGS-P00021 | Ikhithi yokufumana ubungakanani be-SMEDROMS ye-hypesculotis yesifo sephepha | Ukuchongwa okungabhaliswanga (ulwelo lwe-biopsy) lokufumana i-hyperveritical ye-pucteria-ne-pulmoary yesifo se-pulmonary sturculosis kunye ne-subles yengqondo; Iisampulu zabantu ekrokrelwa ukuba bosulelwe sisifo sephepha okanye isifo sephepha le-turcobacterias bahlalutywa ngamandla e-megevolosis, kunye nolwazi lokufumana i-tuberculosul okanye i-turculosis ye-tuberculosus okanye i-My-Gen-Gen-Gen-Gen-Guqula le-Mycobacterium banikezelwa. | Igazi le-Peipheral, iAlveolar Lavage, i-Hydrothorax kunye ne-Ascites, isampulu yenzuzo, i-cerebrospinal fluid. | Isizukulwana sesibini |
| I-HWTS-NGS-T001 | I-MyCobacterium yoThengising kunye neKikhi yokuHlanganisa iziyobisi | Uvavanyo lokuchwetheza kwe-Mycobacterium, kubandakanya i-MTBC kunye ne-187 thm;Ukufunyanwa kokunganyangeki kwamachiza kwe-Mycobacterium Tuberculosis igubungela iziyobisi ezili-13 kunye nezi-16 ze-16 zokuguqula utshintsho lwezihlazo. | I-Sputum, i-Alveolar Lavage fluid, i-Hydrothorax kunye ne-ASCITS, i-Fikation Plation, i-cerebrospinal fluid. | Okwesibini / kwisithathu seplanga |
Amagqabantshintshi: I-HWTS-NGS-T001 MyCobacteriacactelium yoThengising kunye neKit yokuHlola kweZiyobisi
Intshayelelo Intshayelelo
Imveliso isekwe kumachiza aphambili kunye neyesibini echazwe kwiZikhokelo zoNyango lwe-TB, i-maccorollies kunye ne-aminoglycoscoscossides isetyenziswa kwizikhokelo zonyango ze-NTM, kunye neendawo zokuchasana neziyobisi zigubungela lonke iqela lemichiza enxulumene neziyobisi kwi Ngubani onesifo se-mycobacterium tuberculo ye-tutarculotium ye-putalisis, kunye nezinye iichiza ezixeliweyo zeziyobisi kunye neendawo zokuguqulela ngokophando kunye neenkcukacha-manani zobomi obukhulu ekhaya kunye phesheya.
Ukuchongwa kotywala kusekwe kwii-NTM ze-NTM zishwankathelwe kwizikhokelo ze-NTM ezipapashwe yijenali yaseTshayina yesifo se-tuberculosis kunye nezibonelelo zokuphefumla kunye nokuvumelana kweengcali. I-pring ekhethiweyo eyileweyo inokukhulisa, ukulandelelana kwaye ichaze ngaphezu kwe-190 yeentlobo ze-NTM.
Ngeetekhnoloji ze-Iscge ejolise kwi-PROLCE, i-genotyping genes kunye nemfuza ye-mycobacterium yaphakanyiswa yi-Andesx Patr, kunye nendibaniselwano ye-Amplican yeGeelis ukuze ifunyanwe ifunyenwe. Iimveliso ezilungiselelwe zona zinokwakhiwa kubungakanani besibini okanye ukufaka isiseko sesithathu sokufaka ilayisensi, kwaye zonke iqonga lesibini kunye neqonga lesithathu linokwenziwa phantsi kwendlela enzulu yokufumana ulwazi olujolise kuyo. Ngokuthelekisa utshintsho olwaziwayo olufumaneka kwidatha eyakhiweyo (kubandakanya i-MyCobacTetersis Tubialogus Turiculogus Stoght Stoght Stoght kunye nokunganyangeki kwamachiza okanye ukuhambelana kweziyobisi ze-Anti-Tuberculosis. Zidityaniswe nesisombululo esivunyiweyo sonyango lwe-sputum ye-Macro & Micro-Micro-Micro-Micro, Ingxaki ye-Incleid ephantsi ye-Spuku ye-Sputum (izihlandlo ezilishumi ngaphezulu kwendlela yemveli) iconjululwe, ukuze itshintshwe i-tracqueded yeziyobisi isetyenziswa ngokuthe ngqo kwiisampulu zeklinikhi.
Uluhlu lweMveliso
34Iphenyane ngokuchaseneyo neziyobisi18iziyobisi ezichasene nesifo sephepha kunye6Iziyobisi ze-NTM zafunyanwa, igubungela297Iindawo ezinganyangekiyo iziyobisi; Iintlobo ezilishumi ze-Mycobacterium Tuberculosis kunye nangaphezuluI-190Iindidi ze-NTM zifunyenwe.
Itheyibhile 1: Ulwazi lwe-18 + 6 Iyobisi + i-190 + NTM
Inzuzo yemveliso
Iisampulu ezomeleleyo zeklinikhi: Iisampulu zesikhohlela zinokufunyanwa ngokuthe ngqo nearhente yokuzimela ngaphandle kwenkcubeko.
Umsebenzi wovavanyo ulula: Umjikelo wokuqala wokusebenza ulula, kwaye ukwakhiwa kwelayibrari kugqityiwe kwiiyure ezi-3, eziphucula ukusebenza kakuhle.
Ukuchwetheza iziyobisi kunye nokuchasana neziyobisi: Ukugubungela iisayithi zokunyathela ze-MTB kunye ne-NTM, ezona zinto zibalulekileyo zekliniki, ukuxhasa iingxelo zohlalutyo ezichanekileyo, kunye nokuvelisa iingxelo zokucoca kunye nokuvelisa iingxelo zokucoca.
Ukuqulunqwa: Imveliso yemveliso, ukuziqhelanisa ne-witstream il kunye ne-MGI / ze-MGI.
Ukuchazwa kwemveliso
| Ikhowudi yemveliso | Igama lemveliso | Iplati yokufumanisa | Inkcazo |
| I-HWTS-NGS-T001 | I-MyCobacterium yoThengising kunye neKikhi yokuHlanganisa iziyobisi | I-Ont, I-Ilnumumina, Mgi, Salus Pro | I-16 / 96RXN |
IXESHA LOKUQALA: UJan-23 ukuya ku-2024