Isixhobo esitsha sokuFunyaniswa kweSifo sePhepha kunye nokuFunyaniswa koKuchasana kweziyobisi: Isizukulwana esitsha sokuSetyenziswa kokuJoliswe (tNGS) kudityaniswe neSifundo soShishino seSifo sePhepha ngoVavanyo lwe-hypersensitivity
Ingxelo yoncwadi: I-CCa: imodeli yokuxilonga esekelwe kwi-tNGS kunye nokufunda komatshini, ofanelekileyo kubantu abanesifo sofuba esincinci kunye ne-meningitis ye-tuberculous.
Isihloko se-Thesis: Ulandelelwano lwesizukulwana esilandelayo esijoliswe kwiTuberculous kunye nokufunda koomatshini: isicwangciso sokuxilongwa kwe-ultra-sensitive ye-paucific pulmonary tubulars kunye ne-meningitis ye-tubular.
Ngamaxesha athile: 《Clinica Chimica Acta》
UKUBA: 6.5
Umhla wokupapashwa: Janyuwari 2024
Idityaniswe neYunivesithi yaseTshayina yeSayensi yeSayensi kunye neSibhedlele saseBeijing Chest yeYunivesithi yezoNyango, iMacro kunye neMicro-Test yaseka imodeli yokuxilongwa kwesifo sephepha esekwe kwisizukulwana esitsha sobuchwephesha obujoliswe ekulandeleleni (tNGS) kunye nendlela yokufunda koomatshini, ebonelela nge-ultra-high. ukufumanisa ubuntununtunu besifo sephepha esineebhaktheriya ezimbalwa kunye ne-meningitis ye-tuberculous, inike indlela entsha yokuxilongwa kwe-hypersensitivity yokuxilongwa kwekliniki yeentlobo ezimbini zesifo sephepha, kwaye yanceda ukuxilongwa ngokuchanekileyo, ukufumanisa ukuxhathisa kumachiza kunye nonyango lwesifo sephepha.Kwangaxeshanye, kufunyaniswe ukuba iplasma cfDNA yesigulana ingasetyenziswa njengohlobo olufanelekileyo lwesampulu yesampulu yeklinikhi kuxilongo lwe-TBM.
Kolu phononongo, iisampulu zeplasma ezingama-227 kunye neesampulu zolwelo lwe-cerebrospinal zisetyenziselwe ukuseka amaqela amabini ekliniki, apho iisampulu zoxilongo lwelabhoratri zasetyenziselwa ukuseka imodeli yokufunda ngomatshini wokuxilongwa kwesifo sephepha, kunye neesampulu zoxilongo lweklinikhi ziye zasetyenziselwa ukuqinisekisa imodeli yokuxilonga.Zonke iisampulu zaqale zajoliswa yiphuli eyilwe ngokukodwa ekujoliswe kuyo ukubamba i-Mycobacterium tuberculosis.Emva koko, ngokusekelwe kwidatha yokulandelelana kwe-TB-tNGS, imodeli yomthi wesigqibo isetyenziselwa ukwenza i-5-fold cross-validation kwi-training and validation seti ye-laboratory diagnostic queue, kunye nemigangatho yokuxilongwa kweesampuli ze-plasma kunye neesampuli ze-cerebrospinal fluid zifunyenwe.Umda ofunyenweyo ungeniswa kwiiseti ezimbini zovavanyo lokuxilongwa kweklinikhi ukuze kubonwe, kwaye ukusebenza kokuxilonga komfundi kuvavanywa yi-ROC curve.Ekugqibeleni, imodeli yokuxilongwa kwesifo sofuba yafunyanwa.
Isazobe soku-1 soyilo loyilo lophando
Iziphumo: Ngokwemilinganiselo ethile ye-CSF yesampuli ye-DNA (AUC = 0.974) kunye nesampuli ye-plasma cfDNA (AUC = 0.908) egqitywe kolu cwaningo, phakathi kweesampuli ze-227, uvakalelo lwesampulu ye-CSF yi-97.01%, i-specific i-95.65%, kwaye uvakalelo kunye neenkcukacha zesampulu yeplasma yayiyi-82.61% kunye ne-86.36%.Kuhlalutyo lweesampulu ezidityanisiweyo ezingama-44 zeplasma cfDNA kunye ne-cerebrospinal fluid DNA evela kwizigulane ze-TBM, isicwangciso sokuxilonga solu phononongo sinokuhambelana okuphezulu kwe-90.91% (40/44) kwiplasma cfDNA kunye ne-cerebrospinal fluid DNA, kwaye ubuntununtunu yi-95.45% (42/44).Kubantwana abanesifo sephepha semiphunga, isicwangciso sokuxilonga solu phononongo sinovakalelo ngakumbi kwiisampuli zeplasma kuneziphumo zokufumanisa i-Xpert yeesampulu zejusi yesisu kwizigulane ezifanayo (28.57% VS 15.38%).
Umzobo 2 Uhlalutyo lwentsebenzo yemodeli yokuxilongwa kwesifo sephepha kwiisampulu zabemi
