Umhlaza wemiphunga usengunobangela ophambili wokufa okubangelwa ngumhlaza kwihlabathi liphela,Umhlaza wemiphunga ongengoweeseli ezincinci (i-NSCLC) umalunga nama-85% azo zonke iimeko.Kangangamashumi eminyaka, unyango lwe-NSCLC ephucukileyo beluxhomekeke kakhulu kwi-chemotherapy, isixhobo esibuthuntu esasinika ukusebenza okulinganiselweyo kunye nobuthi obukhulu.
Utshintsho kunyango lwaqala ngokufunyanwa "kweenguqu zomqhubi" - utshintsho oluthile lwemfuza olukhuthaza ukukhula kwethumba. Oku kwabangela unyango olujoliswe kulo, olusebenza njengemijukujelwa ekhokelwa ngokuchanekileyo, lutshabalalisa iiseli zomhlaza ngokukhetha ngelixa lugcina eziphilileyo. Nangona kunjalo, impumelelo yolu nyango luguqukayo ixhomekeke ngokupheleleyo kuvavanyo lwemfuza oluchanekileyo noluthembekileyo ukuze kuchongwe ithagethi elungileyo yesigulana esifanelekileyo.
Iimpawu zeBiomarkers ezibalulekileyo: i-EGFR, i-ALK, i-ROS1, kunye ne-KRAS
Iibhayomarker ezine zimi njengeentsika ekuxilongweni kwe-molecular ye-NSCLC, zikhokela izigqibo zonyango zomgca wokuqala:
-I-EGFR:Eyona nguqu ixhaphakileyo enokwenzeka, ingakumbi kubantu baseAsia, abangabasetyhini, nabangatshayiyo. I-EGFR tyrosine kinase inhibitors (TKIs) efana ne-Osimertinib iphucule kakhulu iziphumo zezigulane.
-I-ALK:"Utshintsho lwedayimani," lufumaneka kwi-5-8% yeemeko ze-NSCLC. Izigulane ezine-ALK fusion-positive zihlala zisabela kakhulu kwi-ALK inhibitors, ziphumelela ekusindeni ixesha elide.
-I-ROS1:Njengoko isabelana ngokufana kwesakhiwo ne-ALK, le "gugu inqabileyo" iyenzeka kwi-1-2% yezigulane ze-NSCLC. Iindlela zonyango ezisebenzayo ziyafumaneka, nto leyo eyenza ukuba ukufunyaniswa kwayo kube yinto ebalulekileyo.
-I-KRAS:Ngokwembali, utshintsho lwe-KRAS luqhelekile. Ukuvunywa kwakutshanje kwe-KRAS G12C inhibitors kutshintshe le biomarker ukusuka kwi-prognostic marker ukuya kwi-target enokwenzeka, nto leyo eguqula ukhathalelo lwale nxalenye yesigulane.
Ipotifoliyo ye-MMT: Yenzelwe Ukuqiniseka Ngokuxilonga
Ukuze kuhlangatyezwane nesidingo esingxamisekileyo sokuchonga i-biomarker ngokuchanekileyo, i-MMT inikezela ngepotifoliyo yeekhithi zokufumanisa i-PCR zexesha langempela eziphawulwe nge-CE-IVD, nganye yenziwe ngobuchwepheshe obuphambili ukuqinisekisa ukuzithemba kokuxilonga.
1. Ikhithi yokuFumanisa uTshintsho lwe-EGFR
-Ubuchwepheshe be-ARMS obuphuculweyo:Izinto zokuphucula ubunini bomzimba zonyusa ukwanda kwenguqu ethile.
-Ukuphucula i-Enzymatic:Ii-endonucleases zokuthintela zigaya imvelaphi ye-genomic yohlobo lwasendle, ziphucula ulandelelwano lwe-mutant kwaye ziphucula isisombululo.
-Ukuthintela ubushushu:Inyathelo elithile lobushushu linciphisa i-priming engacwangciswanga, nto leyo enciphisa ngakumbi imvelaphi yohlobo lwasendle.
-Iingenelo eziphambili:Uvakalelo olungenakuthelekiswa nanto luye kwi1%i-mutant allele frequency, ukuchaneka okugqwesileyo kunye nolawulo lwangaphakathi kunye ne-UNG enzyme, kunye nexesha elikhawulezayo lokutshintsha malungaimizuzu eli-120.
- Iyahambelana nezombini iisampulu ze-tissue kunye ne-liquid biopsy.
2. Ikhithi yokuFumana iMMT EML4-ALK Fusion
- Uvakalelo oluphezulu:Ifumanisa ngokuchanekileyo utshintsho lwe-fusion kunye nomda ophantsi wokufumanisa iikopi ezingama-20/impendulo.
-Ukuchaneka Okugqwesileyo:Ibandakanya imigangatho yangaphakathi yolawulo lwenkqubo kunye ne-enzyme ye-UNG ukuthintela ukungcola okuqhubekayo, ithintela ngempumelelo izinto ezilungileyo nezingalunganga ezingezizo.
-Elula Nekhawulezayo:Isebenza ngendlela elula nevaliweyo, egqitywe kwimizuzu eli-120.
-Ukuhambelana kwezixhobo:Iyalungiselelwa izixhobo ezahlukeneyo ze-PCR zexesha langempela, inika ukuguquguquka kuyo nayiphi na indlela yokuseta elebhu.
3. Ikhithi yokuFumana iMMT ROS1 Fusion
Uvakalelo oluphezulu:Ibonisa ukusebenza okugqwesileyo ngokufumanisa ngokuthembekileyo iikopi ezingama-20/impendulo yeethagethi ezidityanisiweyo.
Ukuchaneka Okugqwesileyo:Ukusetyenziswa kolawulo lomgangatho wangaphakathi kunye ne-enzyme ye-UNG kuqinisekisa ukuthembeka kwesiphumo ngasinye, kunciphisa umngcipheko wokubika iimpazamo.
Elula Nekhawulezayo:Njengenkqubo yetyhubhu evaliweyo, ayifuni manyathelo anzima emva kokukhulisa. Iziphumo ezichanekileyo nezithembekileyo zifunyanwa kwimizuzu emalunga ne-120.
Ukuhambelana kwezixhobo:Yenzelwe ukuhambelana ngokubanzi noluhlu lweematshini ze-PCR eziqhelekileyo, okwenza kube lula ukuhlanganiswa kwimisebenzi ekhoyo yelabhoratri.
4. Ikhithi yokuFumanisa utshintsho yeMMT KRAS
- Itekhnoloji ye-ARMS ephuculweyo, eqiniswe yi-Enzymatic Enrichment kunye ne-Temperature Blocking.
- Ukuphucula i-Enzymatic:Isebenzisa ii-restriction endonucleases ukuze ikhethe ngokugaya imvelaphi ye-genomic yohlobo lwendalo, ngaloo ndlela iphucula ulandelelwano lwe-mutant kwaye iphucula kakhulu isisombululo sokufumanisa.
-Ukuthintela ubushushu:Ingenisa inyathelo elithile lobushushu ukuze ibangele ukungalingani phakathi kwee-primers ezithile eziguqulweyo kunye neetemplate ze-wild-type, inciphisa ngakumbi imvelaphi kwaye iphucule ukucaciswa.
- Uvakalelo oluphezulu:Ifikelela kwi-1% yokuqonda ukuchongwa kwee-alleles eziguqulweyo, iqinisekisa ukuchongwa kweenguqu ezifumaneka kancinci.
-Ukuchaneka Okugqwesileyo:Imigangatho yangaphakathi edibeneyo kunye ne-enzyme ye-UNG ikhusela kwiziphumo ezilungileyo nezingalunganga ezingezizo.
-Iphaneli ebanzi:Yenziwe ngokufanelekileyo ukuze kube lula ukubhaqwa kwe-KRAS mutations ezisibhozo ezahlukeneyo kwiityhubhu ezimbini zokusabela.
- Elula Nekhawulezayo:Inika iziphumo ezichanekileyo nezithembekileyo malunga nemizuzu eli-120.
- Ukuhambelana kwezixhobo:Ilungelelaniswa ngokulula nezixhobo ezahlukeneyo ze-PCR, ibonelela ngokuguquguquka kwiilabhoratri zeklinikhi.
Kutheni Ukhetha Isisombululo se-MMT NSCLC?
Okupheleleyo: Iseti epheleleyo yeebhayomarker ezine ze-NSCLC ezibaluleke kakhulu.
Ubuchwepheshe obugqwesileyo: Ukuphuculwa kobunini (Ukuphucula i-Enzymatic, Ukuthintela ubushushu) kuqinisekisa ukucaciswa okuphezulu kunye nobuntununtunu apho kubaluleke khona.
Iyakhawuleza kwaye Isebenza Kakuhle: Iprotokholi efanayo yemizuzu eli-120 kuyo yonke ipotifoliyo ikhawulezisa ixesha lonyango.
Iguquguqukayo kwaye iyafikeleleka: Iyahambelana noluhlu olubanzi lweesampuli kunye nezixhobo ze-PCR eziqhelekileyo, nto leyo enciphisa imiqobo yokusetyenziswa.
Isiphelo
Kwixesha le-precision oncology, ukuxilongwa kweemolekyuli yindlela ekhokela ukuzulazula kwezonyango. Iikhithi zokufumanisa eziphambili ze-MMT zinika amandla oogqirha ukuba bakwazi ukudweba ngokuzithemba imeko yemfuza ye-NSCLC yesigulana, bevula amandla okusindisa ubomi onyango olujoliswe kulo.
Contact to learn more: marketing@mmtest.com
Ixesha leposi: Oktobha-14-2025