Utshintsho lwamanqaku kumfuza we-KRAS luchaphazeleka kuluhlu lwamathumba abantu, kunye namazinga otshintsho amalunga ne-17% -25% kuzo zonke iintlobo zethumba, i-15% -30% kumhlaza wemiphunga, kunye nama-20% -50% kumhlaza womlomo. Olu tshintsho luqhuba ukuxhathisa kunyango kunye nokuqhubela phambili kwethumba ngendlela ephambili: iprotein ye-P21 efakwe kwikhowudi yi-KRAS isebenza ezantsi kwendlela yokubonisa i-EGFR. Nje ukuba i-KRAS iguqulwe, iqhubeka ngokuqhubekayo ukubonakaliswa komlambo, inika unyango olujoliswe kwi-EGFR oluphezulu lungasebenzi kwaye lukhokelela ekwandeni kweeseli ezinobungozi. Ngenxa yoko, iinguqulelo ze-KRAS zinxulunyaniswa nokuchasana ne-EGFR tyrosine kinase inhibitors kumhlaza wemiphunga kunye nonyango lwe-anti-EGFR lwe-antibody kumhlaza webala.

Ngo-2008, iNational Comprehensive Cancer Network (NCCN) yaseka izikhokelo zeklinikhi ezicebisa uvavanyo lokuguqulwa kwe-KRAS kuzo zonke izigulane ezinomhlaza we-metastatic colorectal (mCRC) phambi konyango. Izikhokelo ziqaqambisa ukuba uninzi lwe-KRAS olusebenzayo lwenguquko lwenzeka kwi-codons 12 kunye ne-13 ye-exon 2. Ngaloo ndlela, ukufumanisa ukuguqulwa kwe-KRAS ngokukhawuleza kunye nokuchanekileyo kubalulekile ekukhokeleni unyango olufanelekileyo lweklinikhi.

Kutheni uvavanyo lwe-KRAS lubalulekileMetastaticColorectalCumhlaza(mCRC)

Umhlaza weColorectal (CRC) ayisosifo esinye kodwa ingqokelela ye-molecularly distinct subtypes. Iinguqu ze-KRAS-ezikhoyo malunga ne-40-45% yezigulane ze-CRC-zisebenza njengento eqhubekayo "yokuvula", ukukhuthaza ukukhula komhlaza ngaphandle kwezibonakaliso zangaphandle. Kwizigulane ezine-mCRC, isimo se-KRAS simisela ukusebenza kwe-anti-EGFR monoclonal antibodies ezifana ne-Cetuximab kunye ne-Panitumumab:

Uhlobo lwasendle lwe-KRAS:Izigulane kusenokwenzeka ukuba zixhamle kunyango lwe-anti-EGFR.

I-KRAS eguqukayo:Izigulane azifumani nzuzo kwezi arhente, zibeka emngciphekweni iziphumo ebezingalindelekanga ezingeyomfuneko, ukonyuka kweendleko, kunye nokulibaziseka kunyango olusebenzayo.

Uvavanyo oluchanekileyo nolunovakalelo lwe-KRAS ke ngoko sisiseko socwangciso lonyango lomntu.

Umngeni wokuFumana: Ukwahlula uMbonakaliso weNguqu

Iindlela zesiNtu zihlala zingenabuntununtunu botshintsho obuninzi obuphantsi, ngakumbi kwiisampulu ezinomxholo ophantsi wethumba okanye emva kokuchithwa. Ubunzima buxhomekeke ekwahluleni umqondiso we-DNA oguquguqukayo obuthathaka kwimvelaphi yohlobo oluphakamileyo lwasendle—njengokufumana inaliti kwimfumba yefula. Iziphumo ezingachanekanga zingakhokelela kunyango olungachanekanga kunye neziphumo eziphazamisayo.

Isisombululo sethu: Ukuchaneka-kweNjineli yokuQinisekisa ukuGuqulwa koGuquko

Ikhithi yethu yoFundo lweKRAS yoGuquko idibanisa ubugcisa obuphambili ukoyisa le mida, inikezela ngokuchaneka okukhethekileyo kunye nokuthembeka kwisikhokelo sonyango lwe-mCRC.

Indlela iTekhnoloji yethu eqinisekisa ngayo ukusebenza okuPhezulu

• Itekhnoloji eyomeleziweyo ye-ARMS (iNkqubo yokuGuqulwa kwe-Amplification Refractory): Yakha phezu kobuchwephesha be-ARMS, ibandakanya ubuxhakaxhaka bokuphucula ubuchwephesha ukunyusa ubhaqo oluthile.
• Utyetyiswe nge-Enzymatic: Isebenzisa ii-endonucleases ezithintelweyo ukwetyisa uninzi lwemvelaphi yohlobo lwasendle lomntu, ukonga iintlobo eziguqukayo, ngaloo ndlela iphucula ukubonwa kunye nokunciphisa ukukhulisa okungangqalanga ngenxa yemvelaphi ephezulu ye-genomic.
• Ukuvinjwa kobushushu: Yazisa amanyathelo athile obushushu kwinkqubo yePCR, ebangela ukungahambelani phakathi kwe-mutant primers kunye neetemplates zohlobo lwasendle, ngaloo ndlela kuncitshiswe imvelaphi yohlobo lwasendle kunye nokuphucula isisombululo sokubona.
• I-Sensitivity ephezulu: Ibona ngokuchanekileyo isezantsi njenge-1% ye-DNA eguqukayo.
• Ukuchaneka okugqwesileyo: Isebenzisa imigangatho yangaphakathi kunye ne-UNG enzyme ukukhusela iziphumo zobuxoki kunye nezibi.
• Ilula kwaye iKhawulezayo: Igqibezela uvavanyo malunga nemizuzu eyi-120, isebenzisa iityhubhu ezimbini zokuphendula ukwenza lula ukubonwa kotshintsho olusibhozo olwahlukileyo, inika injongo kunye neziphumo ezithembekileyo.
• Ukuhambelana kwesixhobo: Iqhelanisa nezixhobo ezahlukeneyo zePCR.

Iyeza elichanekileyo kumhlaza wesisu liqala ngoxilongo oluchanekileyo lwemolekyuli. Ngokwamkela iKRAS Mutation Detection Kit yethu, ilabhoratri yakho inokunikezela ngeziphumo eziqinisekileyo, ezinokubakho ezibumba indlela yonyango yesigulana.

Xhobisa ilebhu yakho ngetekhnoloji ethembekileyo, ekumgangatho ophezulu-kwaye unike ukhathalelo olulolwakho ngokwenene.

Qhagamshelana nathi: ukuthengisa@mmtest.Com

Funda ngakumbi malunga nokudibanisa esi sisombululo siqhubela phambili kwinkqubo yakho yokuxilonga.

#Colorectal #Cancer #DNA #Mutation #Precision #Targeted #Unyango #Cancer

Ukuvula i-Precision Medicine kwi-Colorectal Cancer

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