Utshintsho lwezikhombisi kwi-gene ye-KRAS lubandakanyeka kuluhlu lwee-tumor zabantu, kunye namazinga otshintsho amalunga ne-17%–25% kwiintlobo zee-tumor, i-15%–30% kumhlaza wemiphunga, kunye ne-20%–50% kumhlaza we-colorectal. Olu tshintsho luqhuba ukunganyangeki konyango kunye nokuqhubela phambili kwe-tumor ngendlela ephambili: iproteni ye-P21 ebhalwe yi-KRAS isebenza ngezantsi kwendlela yokubonisa i-EGFR. Nje ukuba i-KRAS iguqulwe, ihlala isebenza ngokuqhubekayo, okwenza unyango olujoliswe kwi-EGFR oluphezulu lungasebenzi kwaye lukhokelela ekwandeni okuqhubekayo kweeseli ezinobungozi. Ngenxa yoko, utshintsho lwe-KRAS lunxulunyaniswa nokunganyangeki kwi-EGFR tyrosine kinase inhibitors kumhlaza wemiphunga kunye nonyango lwe-anti-EGFR antibody kumhlaza we-colorectal.

Ngowama-2008, iNational Comprehensive Cancer Network (NCCN) yaseka izikhokelo zeklinikhi ezicebisa uvavanyo lwe-KRAS mutation kuzo zonke izigulane ezine-metastatic colorectal cancer (mCRC) ngaphambi kokuba unyango luqale. Ezi zikhokelo zibonisa ukuba uninzi lwe-activated KRAS mutations lwenzeka kwi-codons 12 kunye ne-13 ye-exon 2. Ke ngoko, ukufunyanwa kwe-KRAS mutation ngokukhawuleza nangokuchanekileyo kubalulekile ekukhokeleni unyango olufanelekileyo lweklinikhi.

Kutheni uvavanyo lwe-KRAS lubaluleke kakhulu kwi-Mi-etastaticCi-olorectalCi-ancer(mCRC)

Umhlaza weColorectal (CRC) awusosifo sinye kodwa yingqokelela yee-subtypes ezahlukeneyo zeemolekyuli. Utshintsho lwe-KRAS—olukhoyo malunga nama-40–45% ezigulane ze-CRC—lusebenza njenge-"on" switch eqhubekayo, lukhuthaza ukukhula komhlaza ngaphandle kwemiqondiso yangaphandle. Kwizigulane ezine-mCRC, imeko ye-KRAS imisela ukusebenza kakuhle kwee-anti-EGFR monoclonal antibodies ezifana neCetuximab kunye nePanitumumab:

Uhlobo lweWild-Type KRAS:Izigulane zisenokuxhamla kunyango oluchasene ne-EGFR.

I-KRAS eguqulweyo:Izigulane azifumani nzuzo kwezi arhente, nto leyo ebeka emngciphekweni iziphumo ebezingalindelekanga, iindleko ezongezelelekileyo, kunye nokulibaziseka kunyango olusebenzayo.

Uvavanyo lwe-KRAS oluchanekileyo nolunovelwano ke ngoko lusisiseko socwangciso lonyango lomntu ngamnye.

Umngeni Wokufumanisa: Ukwahlula Isignali Yoguquko

Iindlela zemveli zihlala zingenalo uvakalelo kwiinguqu ezincinci, ngakumbi kwiisampuli ezinomthamo omncinci wethumba okanye emva kokususwa kwecalcium. Ubunzima busekuhlukaniseni isignali ye-DNA eguqukileyo engabonakaliyo ngokuchasene nemvelaphi ephezulu yasendle—njengokufumana inaliti kwinqwaba yengca. Iziphumo ezingachanekanga zinokukhokelela kunyango olungenalwazi kwaye iziphumo zibe sengozini.

Isisombululo Sethu: Yenziwe Ngobuchule Bokufumanisa Uguquko Oluqinisekileyo

I-KRAS Mutation Detection Kit yethu idibanisa ubuchwepheshe obuphambili ukuze boyise le mida, inika ukuchaneka okugqwesileyo kunye nokuthembeka kwisikhokelo sonyango lwe-mCRC.

Indlela iTekhnoloji yethu eqinisekisa ngayo ukusebenza kakuhle

• Iteknoloji ye-ARMS ephuculweyo (iNkqubo yoTshintsho lwe-Amplification Refractory): Yakhelwe phezu kweteknoloji ye-ARMS, ibandakanya iteknoloji yokuphucula eyiyo ukuze kwandiswe ukuchongwa okucacileyo.
• Ukutyebisa ii-Enzymatic: Isebenzisa ii-endonucleases ezithintela ukugaya uninzi lwemvelaphi ye-genome yomntu, igcina iintlobo ze-mutant, ngaloo ndlela iphucula isisombululo sokufumanisa kwaye inciphise ukwanda okungachazwanga ngenxa yemvelaphi ephezulu ye-genomic.
• Ukuthintela ubushushu: Kuzisa amanyathelo athile obushushu kwinkqubo ye-PCR, okubangela ukungalingani phakathi kwee-primers eziguquliweyo kunye neetemplate ze-wild-type, ngaloo ndlela kunciphisa imvelaphi ye-wild-type kwaye kuphuculwe isisombululo sokufumanisa.
• Uvakalelo Oluphezulu: Ibona ngokuchanekileyo i-DNA eguqukileyo engaphantsi kwe-1%.
• Ukuchaneka Okugqwesileyo: Isebenzisa imigangatho yangaphakathi kunye ne-enzyme ye-UNG ukuthintela iziphumo ezilungileyo nezingalunganga ezingezizo.
• Kulula kwaye Kukhawuleza: Igqiba uvavanyo malunga nemizuzu eli-120, isebenzisa iityhubhu ezimbini zokusabela ukuze kube lula ukufumanisa utshintsho olusibhozo olwahlukileyo, nto leyo evelisa iziphumo ezichanekileyo nezithembekileyo.
• Ukuhambelana kwezixhobo: Ihambelana nezixhobo ezahlukeneyo ze-PCR.

Unyango oluchanekileyo kumhlaza we-colorectal luqala ngokuxilongwa ngokuchanekileyo kwe-molecular. Ngokusebenzisa i-KRAS Mutation Detection Kit yethu, ilabhoratri yakho inokubonelela ngeziphumo eziqinisekileyo nezisebenzayo ezibumba ngqo indlela yonyango lwesigulana.

Nika amandla ilebhu yakho ngetekhnoloji ethembekileyo nesemgangathweni—kwaye unike amandla ukhathalelo olukhethekileyo ngokwenene.

Qhagamshelana nathi: ukuthengisa@mmtest.Com

Funda ngakumbi malunga nokudibanisa esi sisombululo siphambili kumsebenzi wakho wokuxilonga.

#Umhlaza #I-DNA #Utshintsho #Ukuchaneka #Ijoliswe #Unyango #Umhlaza

Ukuvula Unyango Oluchanekileyo kwiCancer yeColorectal

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