Uphononongo lwakutshanje lwe-metagenomic lutyhile uluhlu olwahlukeneyo lweentsholongwane kunye neebhaktheriya ezithwalwa ziimpuku kwiPhondo laseHeilongjiang, kuquka i-Lassa virus, i-Amur virus, i-Salmonella, kunye ne-Yersinia pestis.
Uphando lusebenziseIkhithi yokuNyusa i-RNA yePathogen evela kwiMacro kunye neMicro-Test, ngokusekelwe kubuchwepheshe bokubamba iprobe. Ngokususa ngokukhetha i-host rRNA kunye nezinye iziqwenga zomgcini ezigciniweyo, le ndlela igcine i-RNA ye-microbial ngempumelelo, iphucula kakhulu uvakalelo lokufunyanwa kwe-pathogen. Eli nyathelo lokukhulisa i-RNA ye-pathogen lalibalulekile ekufezekiseni izinga eliphezulu lokufunyanwa kwaye labonelela ngenkxaso enamandla yobugcisa yokujonga i-pathogen.
Iimpuku: Iindawo eziphambili zokugcina amanzi kwizifo zezilwanyana
Iimpuku zezona ntlobo zezilwanyana ezincancisayo zininzi kwaye zisasazeke kakhulu, ziqulathe iintlobo ezingaphezu kwama-2,000 kwihlabathi liphela kwaye zingaphezulu kwama-40% azo zonke iintlobo zezilwanyana ezincancisayo.
Ngaphandle kokubaluleka kwazo kwindalo, iimpuku zisebenza njengeendawo zokugcina izilwanyana ezinkulu kunye nezilwanyana ezithwala izifo ezininzi zezilwanyana.Ngenxa yokuba iindawo zokuhlala zeempuku zihlala zidibana neendawo ezihlala abantu—ngokuluma ngqo, ukudibana nelindle, okanye ukudluliselwa ngokungathanga ngqo ngeentakumba kunye neencukuthu—iintsholongwane ezinazo ziyimingcipheko engapheliyo kwimpilo yoluntu.
Okuphawulekayo kukuba, ngaphezu kwama-70% ezifo ezisulelayo ezivelayo zivela kwizilwanyana zasendle, apho iimpuku zimela enye yezona ndawo zibalulekileyo zokugcina iintsholongwane.
Ummandla weMida yeHeilongjiang: Umgca ongaphambili wokujonga iintsholongwane
IHeilongjiang, iphondo elikumda kumantla-mpuma eTshayina, linemithombo emininzi yendalo kwaye lihlala kwimida namazwe amaninzi. Ukudibana kwezinto eziphilayo ezityebileyo kunye nokuhamba rhoqo kwabantu besuka kwamanye amazwe kudala iimeko ezilungileyo zokusasazwa kwezifo.
Phakathi kukaJuni noJulayi 2021, abaphandi baqokelele iimpuku zasendle ezingama-67 kwiDolophu yaseHengdaohezi, kwiSixeko saseMudanjiang. Iisampulu ziquka:
l 20I-Apodemus peninsulae
l 47I-Myodes rufocanus
Zonke iisampulu zabanjwa kwiindawo zokuhlala nakwiifama ezikufutshane, nto leyo ebonisa unxibelelwano olusondeleyo phakathi kweendawo zokuhlala zabantu neendawo zokuhlala zasendle.
Itekhnoloji yokuNyusa iiPathogen: Ukoyisa ukuphazamiseka kwemvelaphi yoMbumbi
Kwi-metagenomic sequencing, i-host RNA idla ngokulawula i-sequencing reads, ifihla imiqondiso ye-pathogen encinci.
Ukujongana nalo mceli mngeni, olu phando lusebenziseIkhithi yokuFumanisa iMetagenomic yeMacro kunye neMicro-Test. Ngokusebenzisa iteknoloji yokubamba iprobe, iiprobe ezenzelwe ngokukodwa zibopha kwi-rRNA ebambeyo kwaye zigcine ulandelelwano, zivumela ukuncipha kwazo kwaye ngaloo ndlela zityebisa i-RNA yeentsholongwane.
Le ndlela iphucule kakhulu uvakalelo lokufumanisa izifo, ivumela ulandelelwano olusezantsi ukuba lufumane inani eliphezulu lokufundwa kwezifo. Inyathelo lokutyebisa labeka isiseko esiqinileyo sokuchaza ngokuchanekileyo uluhlu lwezifo oluthwalwa ziimpuku.
Emva kokutyebisa, iisampulu ze-RNA zenziwe i-reverse transcription, ukwakhiwa kwethala leencwadi, kunye nokulandelelana kwe-high-throughput. Uhlalutyo lwe-Bioinformatic luquke:
l Ukucoca i-genome yoMphathi
Uhlu lwe-taxonomic kusetyenziswa iKraken2
Ukulungiswa kobuninzi ngeBracken
Olu hambo lomsebenzi luvelise iprofayili epheleleyo yeendawo eziphila nentsholongwane kunye neentsholongwane.
Iziphumo zoPhando: Ukujikeleza kwegazi kunye neentsholongwane ezininzi
Iziphumo zeNtsholongwane
Iintsholongwane ezifunyenweyo bezizezeentsapho ezingama-21 zentsholongwane, kuqukaI-ArenaviridaekwayeIHantaviridae.
Ngokudlulade novoUkuhlanganiswa kusetyenziswa iMegahit, kwaveliswa iintsholongwane ezili-154, apho ezili-110 kuzo zazihambelana neentsholongwane ezili-19 ezivela kwiintsapho ezisibhozo.
In I-Apodemus peninsulae, ezi ntsholongwane zilandelayo zichongiwe:
l Arenaviruses: Lassa virus, Wenzhou virus, Gairo virus
l Hantaviruses: Intsholongwane ye-Amur, intsholongwane yePuumala
In I-Myodes rufocanus, I-arterivirus yegundane kunye ne-Amur virus zifunyenwe.
Phakathi kwezi zifo:
Intsholongwane yeLassa yimbangela yomkhuhlane wegazi obangelwa yintsholongwane, apho amazinga okufa kwabantu afikelela kwi-20%.
Intsholongwane ye-Amur yenye yeentsholongwane ezibangela umkhuhlane wegazi ohamba ngegazi kunye nesifo sezintso (i-HFRS). I-China ibika umthwalo ophezulu kwihlabathi liphela wosulelo lwe-hantavirus.
Iziphumo zeBhaktheriya
Izahlulo zebhaktheriya eziyi-1,051 zizonke.
Ezona genera zininzi zezi:
lI-Salmonella(26–31%)
lIYersinia(11–23%)
I-Salmonellayintsholongwane enkulu ebangelwa kukutya, ebangela iimeko eziqikelelwa kwizigidi ezili-9 zomkhuhlane wetyphoid kunye nokufa kwabantu abamalunga ne-110,000 minyaka le kwihlabathi liphela.
IYersinia pestis, imbangela yesifo esibulalayo, ibe negalelo kwiintsholongwane ezintathu zembali. Iphondo laseHeilongjiang laziwa njengenye yezona ndawo zibalulekileyo zaseTshayina ezijongene nesi sifo kwaye lihlala liyindawo ephambili yokujonga esi sifo.
Ukubaluleka koFundo
Igalelo leSayensi
Olu phononongo lubonisa ingxelo yokuqala yeziqwenga zentsholongwane iLassa kunye nentsholongwane ye-arteritis yeempuku kwiimpuku zaseHeilongjiang, nto leyo eyandisa ulwazi lwe-database yezifo ezithwalwa ziimpuku eTshayina.
Ichaza ngokucwangcisiweyo uluntu olunentsholongwane kunye nolwebhaktheriya kwiindawo ezihlala iimpuku, inika idatha esisiseko yokujonga izifo zezilwanyana kwimimandla esemdeni.
Iziphumo zeMpilo yoLuntu
Ezi ziphumo zibonisa imfuneko yokujonga rhoqo kunye nokuhlolwa kwezifoA. peninsulaekwayeM. rufocanus.
Le datha inika ubungqina besayensi obuxhasa amaqhinga okulawula imithombo yezifo ezifana nesifo sombefu kunye nomkhuhlane wegazi ohamba ngegazi one-renal syndrome.
Amandla eNdlela
Ulandelelwano lwe-metagenomic lwenza ukuba kufunyanwe ngokungakhethi cala iintsholongwane ezaziwayo nezintsha.
Kolu phononongo, iteknoloji yokuphucula i-pathogen yeMacro & Micro-Test ibonise ukusebenza kakuhle, iphucula ngempumelelo ukufunyanwa kwe-pathogen ekujoliswe kuyo kwiisampulu zebhayoloji ezintsonkothileyo. Iziphumo ziqinisekisa amandla ayo okuxhasa ukuchonga i-pathogen enobuzaza obuphezulu ekujongeni izilwanyana zasendle.
Ingcebiso yeMveliso
Ngokusekelwe kwisicelo sayo esiphumeleleyo kolu phononongo, i-Macro & Micro-Test Pathogen RNA Enrichment Kit icetyiswa kakhulu kuphando lokujonga i-metagenomic.
Ngokususa ngempumelelo ii-nucleic acids ze-host kunye nokuphucula ulandelelwano lwee-microbial, le khithi iphucula kakhulu uvakalelo lokufumanisa kunye nomgangatho wedatha. Imele isixhobo esibalulekileyo sokufumanisa ngokuchanekileyo i-pathogen kwiisampulu ezintsonkothileyo kwaye inika inkxaso yobugcisa eqinileyo yokujonga impilo yoluntu, uvavanyo lomngcipheko we-zoonotic, kunye nokulungiselela ukuqhambuka.
Contact to learn more: marketing@mmtest.com
Ixesha lokuthumela: Matshi-02-2026