Iindidi ezili-14 zoMngcipheko ophezulu wePapillomavirus yabantu (16/18/52 Ukuchwetheza)
Igama lemveliso
HWTS-CC019A-Iindidi ezili-14 ze-Human Papillomavirus (16/18/52 Typing) zoMngcipheko ophezulu weNucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Uphononongo lubonise ukuba usulelo oluzingisileyo lwe-HPV kunye nosulelo oluninzi ngomnye woonobangela abaphambili bomhlaza womlomo wesibeleko.Okwangoku, unyango olusebenzayo oluvunyiweyo lusanqongophala lomhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nothintelo losulelo lomlomo wesibeleko olubangelwa yi-HPV ngundoqo ekuthinteleni umhlaza womlomo wesibeleko.Kubaluleke kakhulu ukuseka uvavanyo olulula, olucacileyo kunye nolukhawulezayo lwe-etiology yokuxilongwa kwekliniki kunye nonyango lomhlaza womlomo wesibeleko.
Umjelo
| Umjelo | Uhlobo |
| FAM | I-HPV 18 |
| VIC/HEX | I-HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| IQuasar 705/CY5.5 | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Umchamo, iSwab yoMlomo weSibeleko |
| Ct | ≤28 |
| LoD | Iikopi ezingama-300/mL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye nezinye iisampuli zokuphefumla ezifana Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2 I-B1 / B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njl. kunye ne-DNA ye-genomic yabantu. |
| Izixhobo Ezisebenzayo | I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) Inkqubo ye-BioRad CFX96 yeXesha langempela lePCR kunye neBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
1.Isampulu yomchamo
A: Thatha1.4i-mL yesampuli yomchamo ukuba ivavanywe kunye ne-centrifuge kwi-12000rpm imizuzu emi-5;lahla i-supernatant (kucetyiswa ukuba ugcine i-10-20μL ephezulu ukusuka ezantsi kwityhubhu ye-centrifuge), yongeza i-200μL ye-reagent yokukhululwa kwesampulu, kwaye ukukhutshwa okulandelayo kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa kwe-Macro & Micro-Test Sample Release. I-Reagent (HWTS-3005-8).
B: Thatha1.4mL yesampuli yomchamo ukuba ivavanywe kunye ne-centrifuge kwi-12,000rpm imizuzu emi-5;lahla i-supernatant (kucetyiswa ukuba ugcine i-10-20μL ye-supernatant ukusuka ezantsi kwityhubhu ye-centrifuge), kwaye ungeze i-200μL ye-saline eqhelekileyo ukumisa kwakhona, njengesampuli ukuba ihlolwe.Ukukhutshwa okulandelayo kunokuqhutywa kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokusetyenziswa kunye neMacro & I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ngokuhambelana ngokungqongqo nomyalelos ukusetyenziswa.Umthamo we-elution ocetyiswayo yi-80μL.
C: Thatha1.4mL yesampuli yomchamo ukuba ivavanywe kunye ne-centrifuge kwi-12,000rpm imizuzu emi-5;lahla i-supernatant (kucetyiswa ukuba ugcine i-10-20μL ye-supernatant ukusuka ezantsi kwityhubhu ye-centrifuge), kwaye ungeze i-200μL ye-saline eqhelekileyo ukumisa kwakhona, njengesampuli ukuba ihlolwe.Ukutsalwa okulandelayo kunokuqhutywa ngeI-QIAamp DNA Mini Kit (51304) nge-QIAGEN okanye i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa.Umthamo wesampula yokutsalwa yi-200μL, kunye nomthamo we-elution ocetyiswayo ngama-80μL.
2. I-swab yomlomo wesibeleko / isampuli ye-vaginal swab
A: Thatha i-1mL yesampuli ukuba ivavanywe kwi-1.5mLof ityhubhu ye-centrifuge,kwayecentrifuge kwi 12000rpm imizuzu emi-5. Discard i-supernatant (kuyacetyiswa ukuba ugcine i-10-20μL ye-supernatant ukusuka ezantsi kwityhubhu ye-centrifuge), yongeza i-100μL ye-reagent yokukhululwa kwesampulu, uze ukhuphe ngokwemiyalelo yokusetyenziswa kwe-Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Ukukhutshwa kungenziwa nge-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe nge-Macro & I-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ngokuhambelana ngokungqongqo nemiyalelo yokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
C: Ukukhutshwa kungenziwa nge-QIAamp DNA Mini Kit (51304) nge-QIAGEN okanye i-Macro & Micro-Test Viral DNA / RNA Column (HWTS-3020-50).Ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa.Umthamo wesampulu yokutsalwa ngama-200 μL, kunye nomthamo we-elution ocetyiswayo ngu80 μL.
3, I-Swab yoMbane/I-Vaginal Swab
Ngaphambi kokuba wenze isampulu, sebenzisa icotton swab ukosula ngobunono ukukhuselwa okungaphezulu komlomo wesibeleko, kwaye usebenzise enye icotton swab engene nesisombululo sogcino lweseli okanye ibrashi yovavanyo lwesampulu yomlomo wesibeleko ukuze ubambelele kumlomo wesibeleko kwaye ujike imijikelo eyi-3-5 ngasekunene ukuze ufumane. iiseli ezikhutshiweyo zomlomo wesibeleko.Kancinci uthathe i-cotton swab okanye ibrashi,kwayeyifake kwisampulu yetyhubhu ene-1mL ye-saline eqhelekileyo engenazintsholongwane. AEmva kokuhlanjululwa ngokupheleleyo, cinezela yomise i-cotton swab okanye ibrashi kudonga lwetyhubhu kwaye ulahle, qinisa umnqwazi wetyhubhu, kwaye uphawule igama lesampuli (okanye inombolo) kwaye uchwetheze kwityhubhu yesampuli.
