Iintlobo ezili-18 ze-Human Papilloma Virus enobungozi obuphezulu (16/18 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)
Igama lemveliso
Iintlobo ezili-18 ze-Human Papilloma Virus enobungozi obuphezulu (16/18 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Human Papillomavirus (HPV) ikwintsapho yePapillomaviridae yentsholongwane ye-DNA encinci, engagqunywanga, ejikelezayo enemicu emibini, enobude be-genome obumalunga ne-8000 base pairs (bp). I-HPV yosulela abantu ngokudibana ngqo okanye ngokungathanga ngqo nezinto ezingcolisiweyo okanye ukudluliselwa ngokwesondo. Le ntsholongwane ayijoliswanga nje kuphela kumninimzi, kodwa ikwajoliswa nakwizicubu ezithile, kwaye inokosulela kuphela ulusu lomntu kunye neeseli ze-epithelial ze-mucosal, nto leyo ebangela iintlobo ngeentlobo zee-papillomas okanye ii-warts eluswini lomntu kunye nomonakalo okhulayo kwi-epithelium yokuzala.
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zibonise ukuba usulelo oluqhubekayo lwe-HPV kunye nosulelo oluninzi zezinye zezona zizathu ziphambili zomhlaza womlomo wesibeleko. Okwangoku, unyango olusebenzayo oluqatshelweyo alusekho kumhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV yeyona nto iphambili ekuthinteleni umhlaza womlomo wesibeleko. Kubaluleke kakhulu ukumisela uvavanyo olulula, olucacileyo nolukhawulezayo lokuxilonga i-etiology yokuxilongwa kweklinikhi kunye nonyango lomhlaza womlomo wesibeleko.
Itshaneli
| I-Reaction Buffer | Intatheli | Umcimi-mlilo |
| I-18HPV Reaction Buffer | I-FAM | Akukho nanye |
| I-VIC (HEX) | Akukho nanye | |
| I-ROX | Akukho nanye | |
| CY5 | Akukho nanye |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤ -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli yomchamo, Isampuli yeswabhu yomlomo wesibeleko yabasetyhini, Isampuli yeswabhu yomlomo wesibeleko yabasetyhini |
| CV | <5.0% |
| I-LoD | Umda woKuchongwa (i-LoD): I-LoD yekhithi yi-300Copies/mL |
| Ukuchaneka | Akukho mpendulo ihambelanayo nezinye izifo ezinxulumene nendlela yokuzala kunye ne-DNA ye-genomic yomntu. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-SLAN-96P ze-Real-Time PCR (iHongshi Medical Technology Co., Ltd.), iiNkqubo ze-Applied Biosystems 7500 ze-Real-Time PCR, iiNkqubo ze-Applied Biosystems 7500 ze-Fast Real-Time PCR, iiNkqubo ze-QuantStudio®5 ze-Real-Time PCR, iiNkqubo ze-LightCycler®480 ze-Real-Time PCR, iiNkqubo ze-LineGene 9600 Plus ze-Real-Time PCR Detection (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) kunye ne-MA-6000 ye-Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), iiNkqubo ze-BioRad CFX96 ze-Real-Time PCR, iiNkqubo ze-BioRad CFX Opus 96 ze-Real-Time PCR. |
Ukuhamba komsebenzi: Jonga iKit IFU ukuze ufumane iinkcukacha
Isisombululo 1 (Ukukhupha i-nucleic acid ngokuzenzekelayo)
Isisombululo sesi-2: (I-Macro kunye ne-Micro-Test Sample Release Reagent)
Ii-reagents ziyafuneka kodwa azibonelelwa
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), i-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwa
I-1.5mL yeetyhubhu ze-centrifuge ezingenayo i-DNase/RNase, ii-tips ezingenayo i-DNase/RNase, ii-strips ze-8-tube ze-PCR, i-desktop centrifuge, i-desktop vortex mixer.
