Iintlobo ezili-17 ze-HPV (16/18/6/11/44 Uhlobo lokuchwetheza)

Inkcazo emfutshane:

Le khithi ifanelekile ekuchongeni iintlobo ezili-17 ze-human papillomavirus (HPV) (HPV 6, 11, 16,18,31, 33,35, 39, 44,45, 51, 52.56,58, 59,66,68) ezithile ze-nucleic acid fragments kwisampuli yomchamo, isampuli ye-cervical swab yabasetyhini kunye nesampuli ye-vaginal swab yabasetyhini, kunye ne-HPV 16/18/6/11/44 typing ukunceda ekuxilongeni nasekunyangeni usulelo lwe-HPV.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-CC015 Iintlobo ezili-17 zeHuman Papillomavirus (16/18/6/11/44 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)

I-Epidemiology

Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Kuye kwabonakala ukuba usulelo oluqhubekayo lwe-HPV kunye nosulelo oluninzi zezinye zezizathu eziphambili zomhlaza womlomo wesibeleko. Okwangoku kusekho ukunqongophala konyango olusebenzayo olwamkelweyo ngokubanzi lomhlaza womlomo wesibeleko olubangelwa yi-HPV. Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV zizitshixo zokuthintela umhlaza womlomo wesibeleko. Ukusekwa kovavanyo olulula, olucacileyo nolukhawulezileyo lokuxilonga iintsholongwane kubaluleke kakhulu ekuxilongweni komhlaza womlomo wesibeleko ngokwezonyango.

Itshaneli

I-PCR-Mix1	I-FAM	18
	I-VIC/HEX	16
	I-ROX	31,33,35,39,45,51,52,56,58,59,66,68
	CY5	Ulawulo lwangaphakathi

I-PCR-Mix2	I-FAM	6
	I-VIC/HEX	11
	I-ROX	44
	CY5	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	Isampuli yomchamo, isampuli yeswabhu yomlomo wesibeleko yowesifazane, isampuli yeswabhu yowesifazane engaphantsi komfazi
Ct	≤28
I-LoD	Iikopi ezingama-300/mL
Ukuchaneka	Akukho ntsholongwane isasazekayo kwi-Ureaplasma urealyticum, i-Chlamydia trachomatis yendlela yokuzala, i-Candida albicans, i-Neisseria gonorrhoeae, i-Trichomonas vaginalis, i-Mold, i-Gardnerella kunye nezinye iintlobo ze-HPV ezingagutyungelwanga yikhithi.
Izixhobo Ezisebenzayo	Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler^®Iinkqubo ze-PCR zeXesha langempela ezingama-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela

Ukuhamba komsebenzi

I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200μL ye-saline eqhelekileyo ukuze uphinde umise i-pellet kwinyathelo lesi-2.1, kwaye emva koko ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wokucoca ocetyiswayo yi-80μL.

I-reagent yokukhupha ecetyiswayo: I-QIAamp DNA Mini Kit (51304) okanye i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Yongeza i-200μL ye-saline eqhelekileyo ukuze uphinde umise i-pellet kwinyathelo lesi-2.1, uze emva koko ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wesampulu ekhutshiweyo weesampuli zonke ziyi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-100μL.

I-reagent yokukhupha ecetyiswayo: I-Reagent yokukhupha isampulu yeMacro kunye neMicro-Test (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Yongeza i-200μL ye-reagent yokukhupha isampulu ukuze uphinde umise i-pellet kwinyathelo lesi-2.1, uze emva koko ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha.

Ngaphambili: I-Borrelia Burgdorferi Nucleic Acid

Okulandelayo: I-Mycobacterium Tuberculosis Nucleic Acid kunye neRifampicin(RIF)，Resistance(INH)

Bhala umyalezo wakho apha uze uwuthumele kuthi