Iintlobo ezili-17 ze-HPV (16/18/6/11/44 Ukuchwetheza)
Igama lemveliso
HWTS-CC015 17 Iindidi ze-Human Papillomavirus (16/18/6/11/44 Ukuchwetheza) IKit ye-Nucleic Acid Detection (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kwindlela yokuzala yabasetyhini.Kuye kwaboniswa ukuba usulelo lwe-HPV oluzingisileyo kunye nosulelo oluninzi ngomnye woonobangela abaphambili bomhlaza womlomo wesibeleko.Okwangoku kusekho ukunqongophala konyango olwamkelekileyo jikelele lomhlaza womlomo wesibeleko obangelwa yi-HPV.Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV zizitshixo zokuthintela umhlaza womlomo wesibeleko.Ukusekwa kweemvavanyo ezilula, ezicacileyo kunye nezikhawulezayo zokuxilonga iintsholongwane zibaluleke kakhulu kuxilongo lwezonyango lomhlaza womlomo wesibeleko.
Umjelo
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ulawulo lwangaphakathi |
| I-PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isampulu yomchamo, isampulu yeswab yomlomo wesibeleko yabasetyhini, isampulu yeswabhu yelungu lobufazi |
| Ct | ≤28 |
| LoD | 300Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye Ureaplasma urealyticum, Chlamydia trachomatis of reproductive tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold , Gardnerella kunye nezinye iindidi ze-HPV ezingafakwanga kwikiti. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®IiNkqubo ze-PCR ze-480 zeXesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200μL ye-saline eqhelekileyo ukumisa kwakhona i-pellet kwinqanaba le-2.1, kwaye ke ukutsalwa kufuneka kuqhutywe ngokuhambelana kwimiyalelo yokusetyenziswa kwesi sixhobo sotsalo.Umthamo we-elution ocetyiswayo yi-80μL.
I-reagent yokutsalwa ecetyiswayo: QIAamp DNA Mini Kit (51304) okanye iMacro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Yongeza i-200μL ye-saline eqhelekileyo ukumisa kwakhona i-pellet kwinqanaba le-2.1, kwaye emva koko ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa kwesi sixhobo sokukhupha.Umthamo wesampulu okhutshiweyo weisampulu zonke ziyi-200μL, kwaye umthamo we-elution ocetyiswayo yi-100μL.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test yeSample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Yongeza i-200μL ye-reagent yokukhululwa kwesampuli ukumisa kwakhona i-pellet kwinqanaba le-2.1, kwaye emva koko ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa kwesi sixhobo sokukhupha.
