I-Borrelia Burgdorferi Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-OT076 Borrelia Burgdorferi Nucleic Acid (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Isifo seLyme sibangelwa kusulelo lweBorrelia burgdorferi kwaye sidluliselwa kakhulu phakathi kwezilwanyana ezihlala kuzo, phakathi kwezilwanyana ezihlala kuzo kunye nabantu ngamakhakhayi aqinileyo. I-pathogen iBorrelia burgdorferi inokubangela i-Erythema chronicum migrans yabantu, kunye nezifo ezibandakanya iinkqubo ezininzi ezifana nentliziyo, imithambo-luvo, kunye namalungu, njl.njl., kwaye iimpawu zeklinikhi zahlukile. Ngokwendlela ezikhula ngayo izifo, inokwahlulwa ibe lusulelo lwasekhaya kwangoko, usulelo olusasazekileyo oluphakathi kunye nosulelo oluhlala luhleli emva kwexesha, oluyingozi enkulu kwimpilo yoluntu. Ke ngoko, ekuxilongweni kweklinikhi kweBorrelia burgdorferi, kubaluleke kakhulu ukuseka indlela elula, ecacileyo nekhawulezayo yokuxilongwa kweBorrelia burgdorferi.
Itshaneli
| I-FAM | I-DNA yeBorrelia burgdorferi |
| I-VIC/HEX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli yegazi elipheleleyo |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-QIAamp DNA Blood Midi Kit nguQiagen (51185).It kufuneka ikhutshwengokuhambelana ngqongqokwimiyalelo, kwaye umthamo wesisombululo ocetyiswayo ngu100μL.
Ukhetho 2.
IgaziGi-DNA ye-enomicEIkhithi yokukhupha (DP318,Hayi.: JingchangIrekhodi yesixhobo20210062) iveliswe yiTiangen Biochemical Technology (Beijing) Co., Ltd.. It kufuneka ikhutshwengokuhambelana ngqongqokwimiyalelo, kwaye umthamo wesisombululo ocetyiswayo ngu100μL.
Ukhetho 3.
I-Wizard® Genomic DNA Purification Kit (A1120) yiPromega.It kufuneka ikhutshwengokuhambelana ngqongqokwimiyalelo, kwaye umthamo wesisombululo ocetyiswayo ngu100μL.
