Borrelia Burgdorferi Nucleic Acid
Igama lemveliso
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Isifo seLyme sibangelwa lusulelo lweBorrelia burgdorferi kwaye sisasazwa ikakhulu phakathi kwezilwanyana, phakathi kwezilwanyana kunye nabantu ngamakhalane aqinileyo. I-pathogen i-Borrelia burgdorferi inokubangela i-Erythema chronicum migrans, kunye nezifo ezibandakanya iinkqubo ezininzi ezifana nentliziyo, i-nerve, kunye ne-joint, njl., kwaye ukubonakaliswa kweklinikhi kuyahluka. Ngokubhekiselele ekuphuhliseni izifo, zinokwahlulwa zibe lusulelo lwendawo yokuqala, usulelo olusasazwayo oluphakathi kunye nosulelo oluzingileyo kade, oluyingozi kakhulu kwimpilo yabemi. Ke ngoko, kuxilongo lweklinikhi lweBorrelia burgdorferi, kubaluleke kakhulu ukuseka indlela elula, ekhethekileyo kunye nekhawulezayo yokuxilongwa kwe-etiological ye-Borrelia burgdorferi.
Umjelo
| FAM | I-DNA ye-Borrelia burgdorferi |
| VIC/HEX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isampuli yegazi lonke |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Iinkqubo zePCR ze-ABI 7500 ABI 7500 Fast Real-Time PCR Systems QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
QIAamp DNA Blood Midi Kit ngu Qiagen (51185).It kufuneka ikhutshwengokungqinelana ngokungqongqoukuya kumyalelo, kunye nomthamo we-lution ocetyiswayo100μL.
Ukhetho lwesi-2.
IgaziGiDNA enobuninziEikhithi yokukhupha (DP318,Hayi.: JingchangIsixhobo Record20210062) eveliswe yi-Tiangen Biochemical Technology (Beijing) Co., Ltd.. It kufuneka ikhutshwengokungqinelana ngokungqongqoukuya kumyalelo, kunye nomthamo we-lution ocetyiswayo100μL.
Ukhetho lwesi-3.
I-Wizard® Genomic DNA Purification Kit (A1120) yiPromega.It kufuneka ikhutshwengokungqinelana ngokungqongqoukuya kumyalelo, kunye nomthamo we-lution ocetyiswayo100μL.
