I-Borrelia Burgdorfer nucleid acid
Igama lemveliso
I-HWTS-OT076 Borrelia Burgdorfer iKid yokufumanisa ikhithi (iFluorescence PCR)
Isatifiketi
CE
Isipikumu
Isifo se-lyme sibangelwa sisifo kwi-borrelia Burgdorferni kwaye ikakhulu idluliselwa phakathi kwezinja zeZilwanyana, phakathi kwezilwanyana ezimbayo kunye nabantu ngamakhreyithi enzima. I-Pathogen Borrelia Burgdorferni inokubangela i-Erythema yoMntu, kunye nezifo ezibandakanya iinkqubo ezininzi ezinje ngentliziyo, i-nerve, kunye nokuboniswa kwezonyango kuyahluka. Ngokwesiqhelo kwezifo, inokwahlulwa kusulelo lwasekhaya lwasekhaya, ukusula okuphakathi kunye nokuqhubeka kosulelo, olunamandla kwimpilo yabantu. Ke ngoko, kwisifo sekliniki saseBorrelia Burgdorferni, okona kubaluleke kakhulu ukuseka indlela elula, eyodwa nekhawulezayo kunye nekhawulezayo yokufumana isifo se-borrelia Burgdorferferferfer.
Ijelo
Indlala | I-DNA yeBorrelia Burgdorferfe |
I-vic / hex | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
Ukugcinwa | ≤-18 ℃ |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo locaphu | Isampulu yegazi liphela |
Tt | ≤38 |
CV | ≤5.0% |
Indawo yokuhlala | Iikopi ezingama-500 / ml |
Izixhobo ezifanelekileyo | I-ABI 7500 ye-PCR ye-PCR ye-PCR I-ABI 7500 ekhawulezayo ye-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR Ukukhanya®I-480 yeyokwenene ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
I-Qiaamp DNA igazi le-midi kit ye-qiagen (51185).It kufuneka ikhutshwengokuhambelana ngqoKumyalelo, kunye nevolumu yesiqhelo10I-0μl.
Inketho 2.
IgaziGI-DNAEI-titraction kit (DP318,Hayi.: UJingchangIrekhodi yefayileI-20210062) Iveliswa yiTekhnoloji ye-biangen biochemical (Beijing) Co., Ltd.. It kufuneka ikhutshwengokuhambelana ngqoKumyalelo, kunye nevolumu yesiqhelo10I-0μl.
Inketho 3.
I-Wizard ® i-DNA yokucoca iKit (A1120) nge-promega.It kufuneka ikhutshwengokuhambelana ngqoKumyalelo, kunye nevolumu yesiqhelo10I-0μl.