Isixhobo sokuchongwa kwe-antibody ye-Chikungunya Virus IgM/IgG (igolide yeColloidal)
Igama lemveliso
Isixhobo sokuchongwa kwe-antibody ye-Chikungunya Virus IgM/IgG (igolide yeColloidal)
I-Epidemiology
I-Chikungunya sisifo esosulelayo esibangelwa yi-chikungunya virus (CHIKV), esidluliselwa ziingcongconi ze-Aedes, kwaye sibonakaliswa ngumkhuhlane, ukurhawuzelela kunye nentlungu yamalungu. I-Chikungunya yaqinisekiswa ukuba ixhaphakile eTanzania ngo-1952, kwaye intsholongwane yafunyanwa ngo-1956. Esi sifo sixhaphake kakhulueAfrika nakuMzantsi-mpuma weAsia, kwaye ibangele ubhubhane omkhulu kummandla woLwandlekazi lwaseIndiya kwiminyaka yakutshanje [1]. Intsholongwane ye-chikungunya ine-oneerotype kwaye inokwahlulwa ibe zii-genotypes ezintathu, ezizezi uhlobo lwaseNtshona Afrika, uhlobo oluphakathi-mpuma-mpuma-Mzantsi Afrika kunye nohlobo lwase-Asia [2]. Intsholongwane ye-chikungunya ngoku ifunyenwe kumazwe angaphezu kwe-110 e-Asia, e-Afrika, eYurophu naseMelika [3]. Iimpawu zeklinikhi zesifo ziyafana nezo zomkhuhlane we-dengue, kwaye kulula ukuxilonga ngendlela engafanelekanga. Nangona izinga lokufa liphantsi kakhulu, kulula ukwenza ukuqhambuka okukhulu kunye nobhubhane kwiindawo ezinobuninzi beengcongconi [4]. Ii-antibodies ze-IgM kunye ne-IgG kwigazi elipheleleyo lomntu, i-serum, okanye iisampulu zeplasma, kwaye zingasetyenziswa njengesixhobo sokuncedisa sokuxilonga usulelo lwentsholongwane ye-chikungunya kwangoko kunyango. Le khithi ifanelekile ekufumaneni ii-antibodies ze-Chikungunya kubantu ababonisa iimpawu ezithile.
Iiparameters zobugcisa
| Indawo yokugcina izinto | 4~30℃ |
| Beka ubomi kwishelufa | Iinyanga ezingama-24 |
| Uhlobo lweSibonelelo | I-serum, iplasma, kunye neesampuli zegazi elipheleleyo |
| I-LoD | Ukufunyanwa kwe-IgM: uvakalelo 97.56%, ukucaca98.74%, izinga elipheleleyo lokudibana kwezinto liyi-98.50%. Ukufunyanwa kwe-IgG: uvakalelo 97.67%, ukucaciswa 98.73%, izinga elipheleleyo lokungaqondani 98.50%. |
| Ukuchaneka | Akukho reactivity edibeneyo ebonwe kwiimvavanyo ze-cross-reactivity zale khithi eneIntsholongwane ye-encephalitis yaseJapan, intsholongwane ye-encephalitis ethwalwa ziikhakhayi, umkhuhlane omkhulu kunye intsholongwane ye-thrombocytopenia syndrome, intsholongwane ye-Xinjiang hemorrhagic fever, IHantavirus, iDengue virus, iHepatitis C virus, iInfluenza A virus kunye neInfluenza Intsholongwane ye-B. |
Ukuhamba komsebenzi:
Nceda ufunde incwadi yemiyalelo ngononophelo ngaphambi kokuvavanya. Ngaphambi kokuvavanya, khupha zonke ii-reagents kunye neesampuli eziza kuvavanywa kwiimeko zokugcina uze uzivumele zibuyele kubushushu begumbi. Uvavanyo kufuneka lwenziwe kubushushu begumbi.
Iisampuli ze-serum, i-plasma, kunye negazi elipheleleyo:
1. Khupha ikhadi lovavanyo kwingxowa yefoyile ye-aluminiyam uze ulibeke kwindawo ethe tyaba neyomileyo;
2. Sebenzisa i-pipette ukongeza ithontsi eli-1 le-serum, i-plasma, okanye igazi elipheleleyo (malunga ne-10µL) kumngxuma wesampuli "S";
3. Emva koko yongeza amathontsi ama-2 e-diluent yesampuli (malunga ne-70 µL) kwiqula lesampuli "S";
4. Yenza isigwebo sesiphumo kwimizuzu eli-15-20. Isiphumo esiboniswe emva kwemizuzu engama-20 asisebenzi.
