I-Dengue Virus I/II/III/IV Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-Nucleic Acid ye-HWTS-FE034-Dengue Virus I/II/III/IV (i-Fluorescence PCR)
I-HWTS-FE004-Isixhobo sokuFumanisa i-Nucleic Acid ye-Dengue eyomileyo eqandisiweyo (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Umkhuhlane weDengue (DF), obangelwa lusulelo lweDenguevirus (DENV), sesinye sezifo ezosulelayo ze-arbovirus ezixhaphakileyo. I-DENV ikwi-flavivirus phantsi kwe-flaviviridae, kwaye inokwahlulwa ibe zii-serotypes ezi-4 ngokwe-antigen yomphezulu. Indlela yayo yokudlulisela iquka i-Aedes aegypti kunye ne-Aedes albopictus, exhaphake kakhulu kwiindawo ezishushu nezishushu.
Iimpawu zonyango zosulelo lwe-DENV ziquka intloko ebuhlungu, umkhuhlane, ubuthathaka, ukwanda kwe-lymph node, i-leukopenia njl.njl., kunye nokopha, ukothuka, ukwenzakala kwesibindi okanye nokufa kwiimeko ezinzima. Kwiminyaka yakutshanje, utshintsho lwemozulu, ukufudukela kwabantu ezidolophini, uphuhliso olukhawulezileyo lwezokhenketho kunye nezinye izinto ziye zabonelela ngeemeko ezikhawulezayo nezilula zokusasaza nokusasazeka kwe-DF, nto leyo ekhokelela ekwandeni rhoqo kwendawo ebhubhileyo ye-DF.
Itshaneli
| I-FAM | Intsholongwane yeDengue I |
| I-VIC(HEX) | Intsholongwane yeDengue II |
| I-ROX | Intsholongwane yeDengue III |
| CY5 | Intsholongwane yeDengue IV |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ ebumnyameni; i-lyophilization: ≤30℃ ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9; i-lyophilization: iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Serum entsha |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-500/mL |
| Ukuchaneka | Yenza uvavanyo lwe-cross reaction yentsholongwane ye-encephalitis yaseJapan, intsholongwane ye-Forest encephalitis, umkhuhlane onzima one-thrombocytopenia syndrome, umkhuhlane we-Xinjiang hemorrhagic, intsholongwane ye-hantaan, intsholongwane ye-hepatitis C, intsholongwane ye-influenza A, intsholongwane ye-influenza B njl. Akukho mpendulo ifunyenweyo |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Isisombululo se-PCR iyonke
