I-Enterovirus Universal, EV71 kunye ne-CoxA16 Nucleic Acid
Igama lemveliso
Ikhithi yokuFumana i-HWTS-EV010-Enterovirus Universal, EV71 kunye neCoxA16 Nucleic Acid (iFluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla sisifo esosulelayo esibangelwa zii-enterovirus. Okwangoku, kufunyenwe ii-serotypes ezili-108 zee-enterovirus, ezahlulwe zangamaqela amane: A, B, C kunye no-D. Phakathi kwazo, i-enterovirus EV71 kunye ne-CoxA16 zezona zifo ziphambili. Esi sifo sixhaphake kakhulu kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye sinokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo, kwaye inani elincinci labantwana linokubangela iingxaki ezifana ne-myocarditis, i-pulmonary edema, i-aseptic meningoencephalitis, njl.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | Oiiswabhu ze-ropharyngeal,Hiisampulu zolwelo lwe-erpes |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhupha kufuneka kwenziwe ngokwemiyalelo. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
