I-Enterovirus Universal, i-EV71 kunye ne-CoxA16
Igama lemveliso
I-HWTS-EV026B-Enterovirus Universal, EV71 kunye neCoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
I-HWTS-EV020Y/Z-I-Enterovirus Universal, EV71 kunye neCoxA16 Nucleic Acid Detection Kit eyomileyo eqandisiweyo (Fluorescence PCR)
Isatifikethi
I-CE/MDA（HWTS-EV026）
I-Epidemiology
Isifo somlomo wesandla (i-HFMD) sisifo esosulelayo esixhaphakileyo ebantwaneni. Sidla ngokuvela kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye sinokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo, kwaye inani elincinci labantwana linokubangela iingxaki ezifana ne-myocarditis, i-pulmonary edema, i-aseptic meningoencephalitis, njl. Abantwana ngabanye abanezifo ezinzulu bayawohloka ngokukhawuleza kwaye basengozini yokufa ukuba abanyangwa ngokukhawuleza.
Okwangoku, kufunyenwe ii-serotypes ezili-108 zee-enteroviruses, ezahlulwe zibe ngamaqela amane: A, B, C kunye no-D. Ii-Enteroviruses ezibangela i-HFMD zahlukile, kodwa i-enterovirus 71 (EV71) kunye ne-coxsackievirus A16 (CoxA16) zezona zixhaphakileyo kwaye ukongeza kwi-HFMD, zinokubangela iingxaki ezinkulu zenkqubo ye-nervous system ezifana ne-meningitis, i-encephalitis, kunye ne-acute flaccid paralysis.
Itshaneli
| I-FAM | I-Enterovirus |
| I-VIC (HEX) | I-CoxA16 |
| I-ROX | EV71 |
| CY5 | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ EbumnyameniUkwenziwa kwe-Lyophilization: ≤30℃ |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9I-Lyophilization: iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli ye-throat swab, i-Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo.Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Isisombululo se-PCR iyonke
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhupha kufuneka kwenziwe ngokwemiyalelo yesikhokelo. Iisampulu zokukhupha zii-oropharyngeal swabs okanye iisampuli ze-herpes fluid zezigulana eziqokelelwe kwindawo. Yongeza ii-swabs eziqokelelweyo ngqo kwi-Macro & Micro-Test Sample Release Reagent, i-vortex kwaye udibanise kakuhle, ubeke kubushushu begumbi imizuzu emi-5, uyikhuphe uze uyijike kwaye udibanise kakuhle ukuze ufumane i-RNA yesampulu nganye.
I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN okanye i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhupha kufuneka kwenziwe ngokuhambelana ngqo nencwadi yemiyalelo.
