I-Enterovirus Universal, i-EV71 kunye ne-CoxA16
Igama lemveliso
I-HWTS-EV026B-Enterovirus Universal, EV71 kunye ne-CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z-I-Enterovirus eyomileyo eyomisiweyo kwi-Universal, EV71 kunye ne-CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
Isatifikethi
CE/MDA (HWTS-EV026)
I-Epidemiology
I-Hand-foot-mouth disease (HFMD) sisifo esosulelayo esixhaphakileyo ebantwaneni. Ikakhulu iyenzeka kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye inokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwamanye amalungu, kwaye inani elincinane labantwana linokubangela iingxaki ezifana ne-myocarditis, i-pulmonary edema, i-aseptic meningoencephalitis, njl njl.
Okwangoku, i-108 serotypes ye-enteroviruses ifunyenwe, eyahlula ngamaqela amane: A, B, C kunye no-D. I-Enteroviruses ebangela i-HFMD iyahlukahluka, kodwa i-enterovirus 71 (EV71) kunye ne-coxsackievirus A16 (CoxA16) zezona zixhaphakileyo kwaye ngaphezu kwe-HFMD, zingabangela i-encephalitis enzulu njenge-encephalitis, i-complication enzulu, i-encephalitis, kunye ne-complication.
Umjelo
| FAM | I-Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameniI-Lyophilization: ≤30℃ |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9I-Lyophilization: iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isampulu ye-swab yomqala, i-Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Iikopi/mL |
| Izixhobo Ezisebenzayo | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike.Iinkqubo zePCR ze-ABI 7500 ABI 7500 Fast Real-Time PCR Systems SLAN-96P Iinkqubo zePCR zeXesha langempela I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela Iinkqubo zePCR zeLightCycler®480 LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Isisombululo sePCR sisonke
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe ngeMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test yeSampuli yokuKhupha iReagent (HWTS-3005-8). Ukukhutshwa kufuneka kuqhutywe ngokuhambelana nencwadana yemiyalelo. Iisampulu zokutsalwa zii-oropharyngeal swabs okanye iisampulu ze-herpes fluid zezigulane eziqokelelwe kwisiza. Yongeza i-swabs eqokelelweyo ngokuthe ngqo kwi-Macro kunye ne-Micro-Test ye-Sample Release Reagent, i-vortex kwaye udibanise kakuhle, ubeke kwindawo yokushisa ye-5 imizuzu, ukhuphe kwaye uguqule kwaye udibanise kakuhle ukufumana i-RNA yesampuli nganye.
I-reagent yokutsalwa ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) nge-QIAGEN okanye i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokuhambelana nencwadana yomyalelo.
