● Isifo Sephepha seMycobacterium
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I-Mycobacterium Tuberculosis Nucleic Acid kunye neRifampicin(RIF)，Resistance(INH)
Le khithi isetyenziselwa ukufumanisa i-Mycobacterium tuberculosis DNA kwi-sputum yomntu, i-solid culture (LJ Medium) kunye ne-liquid culture (MGIT Medium), i-bronchial lavage fluid, kunye ne-mutations kwindawo ye-507-533 amino acid codon (81bp, i-rifampicin resistance determining region) ye-rpoB gene ye-Mycobacterium tuberculosis rifampicin resistance, kunye ne-mutations kwiindawo eziphambili ze-mutation ze-Mycobacterium tuberculosis isoniazid resistance. Inceda ekuxilongweni kosulelo lwe-Mycobacterium tuberculosis, kwaye ifumanisa ii-gene eziphambili ze-rifampicin kunye ne-isoniazid, ezinceda ukuqonda ukuxhathisa amayeza kwe-Mycobacterium tuberculosis eyosulelwe sisigulana.
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Uguquko lweMycobacterium Tuberculosis INH
Le khithi ifanelekile ekufumaneni iindawo eziphambili zotshintsho kwizampulu zesikhohlela zabantu eziqokelelwe kwizigulane ezineTubercle bacillus ezikhokelela kwisifo sephepha se-mycobacterium INH: Ummandla we-promoter we-InhA -15C>T, -8T>A, -8T>C; Ummandla we-promoter we-AhpC -12C>T, -6G>A; i-homozygous mutation ye-KatG 315 codon 315G>A, 315G>C.
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Isifo Sephepha iMycobacterium Ukumelana neRifampicin
Le khithi ifanelekile ekufumaneni i-homozygous mutation kwi-507-533 amino acid codon region ye-rpoB gene ebangela ukuxhathisa kweMycobacterium tuberculosis rifampicin.
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I-Mycobacterium Tuberculosis Nucleic Acid kunye neRifampicin Resistance
Le khithi ifanelekile ekufumaneni iMycobacterium tuberculosis DNA kwiisampuli zesputum zomntu kwi-vitro, kunye ne-homozygous mutation kummandla we-507-533 amino acid codon we-rpoB gene ebangela ukuxhathisa kweMycobacterium tuberculosis rifampicin.
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I-DNA yeMycobacterium Tuberculosis
Ifanelekile ukufumanisa iMycobacterium tuberculosis DNA ngokwemigangatho yayo kwiisampuli zesputum zabantu, kwaye ifanelekile ukufumanisa isifo seMycobacterium tuberculosis ngokwemigangatho yayo.
