● I-Mycobacterium Tuberculosis
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I-Mycobacterium Tuberculosis Nucleic Acid kunye ne-Rifampicin(RIF)，Ukumelana(INH)
Le kiti isetyenziselwa ukufumanisa umgangatho we-in vitro we-Mycobacterium tuberculosis DNA kwisikhohlela somntu, inkcubeko eyomeleleyo (LJ Medium) kunye nenkcubeko yolwelo (MGIT Medium), ulwelo lokuhlamba lwe-bronchial, kunye notshintsho kwingingqi ye-507-533 amino acid yecodon (81bp, rifampicin ukuxhathisa i-rifampicin emisela ummandla we-rifampicin kwi-general resistance, i-rifampicin emisela ummandla we-tubercoBctea) ngokunjalo notshintsho kwiindawo eziphambili zoguqulo lweMycobacterium tuberculosis isoniazid resistance.Ibonelela ngoncedo ekuxilongeni usulelo lwesifo sephepha seMycobacterium, kwaye ichonge olona hlobo lofuzo lokuxhathisa lwe-rifampicin kunye ne-isoniazid, olunceda ukuqonda ukuxhathisa ngamachiza kwi-Mycobacterium tuberculosis eyosulelwe sisigulana.
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Mycobacterium Tuberculosis INH Ukuguqulwa
Le khithi ilungele ukubhaqwa komgangatho weendawo eziphambili zoguqulo kwiisampulu zesikhohlela somntu eziqokelelwe kwizigulane ezine-Tubercle bacillus positive ezikhokelela kwi-mycobacterium tuberculosis INH: ingingqi ye-InhA yomgqugquzeli -15C>T, -8T>A, -8T>C; Ummandla womgqugquzeli we-AhpC -12C>T, -6G>A; ukuguqulwa kwe-homozygous ye-KatG 315 codon 315G>A, 315G>C.
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I-Mycobacterium Tuberculosis Rifampicin Resistance
Le khithi ilungele ukufunyaniswa komgangatho wenguqu ye-homozygous kwingingqi ye-507-533 ye-amino acid ye-codon yejini ye-rpoB ebangela ukuxhathisa kwe-Mycobacterium tuberculosis rifampicin.
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I-Mycobacterium Tuberculosis Nucleic Acid kunye neRifampicin Resistance
Le khithi ilungele ukufunyaniswa okusemgangathweni kwe-Mycobacterium tuberculosis DNA kwiisampulu zesikhohlela somntu kwi-vitro, kunye nokuguqulwa kwe-homozygous kwindawo ye-507-533 ye-amino acid ye-codon yejini ye-rpoB ebangela ukuxhathisa kwe-Mycobacterium tuberculosis rifampicin.
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I-Mycobacterium Tuberculosis DNA
Ilungele ukufunyaniswa okusemgangathweni kwe-Mycobacterium tuberculosis DNA kwiisampulu zesikhohlela seklinikhi yabantu, kwaye ifanelekile kuxilongo oluncedisayo losulelo lwesifo sephepha seMycobacterium.
