I-DNA yeMycobacterium Tuberculosis
Igama lemveliso
Ikhithi yokuFumanisa i-DNA ye-HWTS-RT001-Mycobacterium Tuberculosis (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Mycobacterium culosis ibizwa ngokuba yiTubercle bacillus (TB). I-Mycobacterium tuberculosis ebangela izifo ebantwini ngoku ithathwa njengeyohlobo lwabantu, iinkomo, kunye neentlobo zaseAfrika. I-pathogenicity yayo inokunxulunyaniswa nokudumba okubangelwa kukwanda kweebhaktheriya kwiiseli zezicubu, ubuthi beenxalenye zebhaktheriya kunye ne-metabolites, kunye nomonakalo womzimba kwiinxalenye zebhaktheriya. Izinto ezibangela izifo zinxulunyaniswa neekhaphu, ii-lipids kunye neeproteni.
Isifo sephepha iMycobacterium sinokuhlasela izinto ezinobungozi ngokusebenzisa indlela yokuphefumla, indlela yokugaya ukutya okanye ukwenzakala kwesikhumba, nto leyo ebangela isifo sephepha kwizicubu ezahlukeneyo kunye namalungu omzimba, apho esiqhelekileyo sisifo sephepha semiphunga ngokusebenzisa indlela yokuphefumla. Sidla ngokuvela ebantwaneni, kwaye sibonakalisa iimpawu ezifana nomkhuhlane ophantsi, ukubila ebusuku, kunye nenani elincinci le-hemoptysis. Usulelo lwesibini lubonakala kakhulu njengomkhuhlane ophantsi, ukubila ebusuku, kunye ne-hemoptysis. Ngokuyintloko sisifo esinganyangekiyo esihlala ixesha elide. Ngo-2018, malunga nezigidi ezili-10 zabantu kwihlabathi liphela bosulelwe sisifo sephepha iMycobacterium, apho malunga nezigidi ezili-1.6 zafa.
Itshaneli
| I-FAM | I-Target (IS6110 kunye ne-38KD) i-nucleic acid DNA |
| I-VIC (HEX) | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-sputum |
| Ct | ≤39 |
| CV | I-Lyophilized: ≤5.0％,Ulwelo: ＜5.0% |
| I-LoD | Ibhaktheriya e-1/mL |
| Ukuchaneka | Akukho kudibana kwe-genome yomntu kunye nezinye izifo ezingezizo ze-Mycobacterium tuberculosis kunye ne-pneumonia |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, i-BioRad Inkqubo yePCR yeXesha langempela yeCFX Opus 96 |
Isisombululo se-PCR iyonke
Ukhetho 1.
Ukhetho 2.
