I-MyCobacterium Tuberculosi stubec acid kunye ne-rifampicin (i-RIF), ukumelana (ish)

I-Mycobacterium tuberculosi stubec acid kunye ne-rifampicin (i-RIF), ukumelana (ih) umfanekiso

Inkcazo emfutshane:

Ikhithi isetyenziselwa i-vitro efanelekileyo yokufumana i-Mycobacterium ye-Mycobacterium yesifo se-Mycobacterium, inkcubeko eqinileyo (inkcubeko eqinileyo) kunye nenkcubeko engamanzi (i-MGIT), kunye notshintsho kwingingqi ye-Amino acid (81bp , I-Rifampampin Ukunganyangeki kwengingqi I-Tuberculosis Rifampining, kunye nokuguqulwa kwezi ndawo zokuhlengahlengiswa kwe-MycobacTerium Tuinasis, kwaye ibonelela ngoncedo lokufumana isifo se-Mycobacterium nase-Imoniazid, encedayo, encedayo ukunyangwa okunganyangekiyo yesifo sephepha le-Mycobacterium bosulelwe sisigulana.

Thumela i-imeyile kuthi

Iinkcukacha zemveliso

Iimpawu zeMveliso

Igama lemveliso

I-HWTS-rt107 I-MyCobacterium tuberculo yesifo se-nucleic acid kunye ne-rifampicin (i-RIF), (i-IH), (i-InH) ikhithi

Isipikumu

Isifo se-MyCobacterium, ngokufutshane njenge-tubercle bacils (i-TB), yibacterium ye-pithogenic ebandakanya i-ruberculosis, kwaye okwangoku, i-anti-tuberculosis isetyenziswa i-isoniazid, i-rifampicin kunye ne-ethambutel, njl^[1]. Nangona kunjalo, ngenxa yokusetyenziswa okungachanekanga kweziyobisi ezichasene ne-anti-tuberculosis kunye neempawu zolwakhiwo lweselfowuni ye-Mycobacterium, i-Mycobacterium ye-MycobacTerium iqulethe iziyobisi kwi-tuberculo yesifo sephepha, i-MDR- I-TB), engamelana nala machiza mabini aqhelekileyo kunye asebenzayo, i-rifampicin kunye isoniazid^[2].

Ingxaki yokungaxhathisi iziyobisi Ukwenzela ukubonelela ngezicwangciso ezichanekileyo zonyango kwizigulana zesifo sephepha, kufuneka ukuba zifumane iziyobisi ezichasene ne-Anti-Tuberculo, ngakumbi inyathelo lokuxilongwa, eliye liyinyathelo lokuphathwa kwe-tuberculosis^[3]. Nangona ukufunyanwa kokufunyanwa kukaRifampini kuxhomekeke ekufumaneni i-MDR-TB, kuphela ekuchanyoneni i-InHicin ngaphandle kwe-rifampicin) kunye ne-monomicant rifampicin (imvakalelo kwi-isoniazid kodwa ukumelana I-Rifampicin), enokukhokelela kwizigulana eziphantsi kwengqondo Iirejimeni zokuqala zonyango. Ke ngoko, kuvavanyo lokunganyangeki kwe-isoniazicicy kunye ne-rifampicin kwaye ubuncinci beemfuno ezifanelekileyo kuzo zonke iinkqubo zokulawula i-DR-TB^[⁴^].

Iiparamitha zobugcisa

Ukugcinwa	≤-18 ℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo locaphu	Isampulu ye-Sputum, inkcubeko eqinileyo (i-LJP), iNkcubeko engamanzi (MGIT ephakathi)
CV	<5.0%
Indawo yokuhlala	Indawo yokuhlala yekhithi yokufumanisa ukuba isifo sephepha se-Mycobacterium yi-10 yentsholongwane / ml;I-ALT yeKit yokufumana udidi lwasendle kunye nohlobo lwe-mguqulo yi-150 iintsholongwane / ml; Indawo yokuhlala yekhithi yokufumanisa uhlobo lwasendle lwase-Imoniazid kunye nohlobo lwe-mguquli yi-200 ye-200 ye-100 yeentsholongwane / ml.
Ubuchule	1) Akukho nto inqamlezayo xa usebenzisa ikhithi ukufumana i-DNA ye-DNA ye-gemic (500ng), ezinye iintlobo ezingama-28 zama-28 iindidi ze-mycobacterias, njengoko kubonisiwe kwiTheyibhile 3).2) Akukho proteyini yomnqamlezo xa usebenzisa ikhithi yokufumana indawo eziguqukayo zenye i-gesis enganyangekiyo ye-rifampicin kunye ne-isoniyazid ye-Itoonizid Mycobacterium Tuberculosis (njengoko kubonisiwe kwiTheyibhile 4).3) Izinto eziqhelekileyo ezixhaphakileyo kwiisampuli zokuvavanywa, ezinje nge-rifampicin (i-9mg / L), i-Isthambullin (i-12mg / l), i-oxymetin (1mg / l), i-MPIROCIN (20mg / l), iPyrazinamide (i-45mg / l), I-Zanamivir (0.5mg / L), DexamethaThasone (20MG / L) iziyobisi, awunampembelelo kwiziphumo zovavanyo lwe-kista.
Izixhobo ezifanelekileyo	I-Slan-96p yangempela yePCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.), I-biorad cfx96 yeyokwenyani ye-PFRR