Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Isoniazid Resistance(INH)

Inkcazelo emfutshane:

Le mveliso ilungele ukufunyaniswa komgangatho we-Mycobacterium tuberculosis DNA kwiisampulu zesikhohlela somntu kwi-vitro, kunye nokuguqulwa kwe-homozygous kwingingqi ye-507-533 ye-amino acid ye-codon (81bp, i-rifampicin emisela ummandla wokumelana) yejini ye-rpoB ebangela isifo sephepha seMycobacterium. ukumelana ne-rifampicin.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Ikhithi yokuFumana (Ijika elinyibilikayo)

I-Epidemiology

I-Mycobacterium tuberculosis, kungekudala njenge-Tubercle bacillus (TB), yibhaktiriya ebangela isifo sephepha.Okwangoku, amachiza alwa nesifo sephepha asetyenziswayo odidi lokuqala aquka isoniazid, rifampicin kunye ne-ethambutol, njl. Nangona kunjalo, ngenxa yokusetyenziswa gwenxa kwamachiza okulwa nesifo sephepha kunye neempawu zesakhiwo sodonga lweseli yesifo sephepha se-mycobacterium, isifo sephepha se-mycobacterium siphuhlisa ukuxhathisa ichiza kumachiza okulwa nesifo sephepha, ezisa imingeni enzima kuthintelo kunye nonyango lwesifo sephepha.

Umjelo

Igama ekujoliswe kulo			Intatheli	Ukucima
Isithinteli sokuphendulaA	Isithinteli sokuphendulaB	Isithinteli sokuphendulaC	Intatheli	Ukucima
rpoB 507-514	rpoB 513-520	IS6110	FAM	Akukho nanye
rpoB 520-527	rpoB 527-533	/	CY5	Akukho nanye
/	/	Ulawulo lwangaphakathi	I-HEX(VIC)	Akukho nanye

Isithinteli sokuphendulaD	Intatheli	Ukucima
Ummandla wokukhuthaza i-InhA -15C>T, -8T>A, -8T>C	FAM	Akukho nanye
KatG 315 codon 315G>A,315G>C	CY5	Akukho nanye
Ummandla womgqugquzeli we-AhpC -12C>T, -6G>A	ROX	Akukho nanye

IiParameters zobuGcisa

Ugcino	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	isikhohlela
CV	≤5.0%
LoD	I-LoD ye-mycobacterium tuberculosis yesizwe ireferensi yi-50 bacteria/mL.I-LoD yohlobo lwasendle oluxhathisa i-rifampicin yi-2×10³ibhaktheriya/mL, kunye ne-LoD yohlobo oluguqukileyo yi-2×10³ibhaktheriya/mL.I-LoD yohlobo lwasendle lwebhaktiriya ekwazi ukumelana ne-isoniazid yi-2x10³ibhaktiriya/mL, kunye ne-LoD yebhaktiriya eguqukayo yi-2x10³ibhaktheriya/mL.
Ukuchaza ngokuthe ngqo	Iziphumo zovavanyo lomnqamlezo zibonise ukuba akukho mpendulo inqamlezileyo ekubhaqweni kwegenome yomntu, ezinye iintsholongwane ze-mycobacteria ezingeyosifo sephepha kunye nenyumoniya ngale khithi;Kwakungekho kusabela okunqamlezileyo okuchongwe kwiindawo eziguqukayo zezinye iijini ezixhathisa ngamachiza kuhlobo lwasendle lweMycobacterium tuberculosis.
Izixhobo Ezisebenzayo	SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, Itekhnoloji ye-Hangzhou Bioer iQuantGene 9600 Inkqubo yePCR yexesha lokwenyani, QuantStudio^®5 Inkqubo yePCR yexesha langempela.