Isifo Sephepha iMycobacterium Ukumelana neRifampicin
Igama lemveliso
Ikhithi yokuHlola ukuKhawuleza kweRifampicin ye-HWTS-RT074A-Mycobacterium Tuberculosis (iFluorescence PCR)
I-Epidemiology
I-Rifampicin ibisetyenziswa kakhulu kunyango lwezigulane ezine-pulmonary tuberculosis ukususela ngasekupheleni kweminyaka yoo-1970, kwaye inefuthe elibalulekileyo. Ibilukhetho lokuqala ukufinyeza i-chemotherapy yezigulane ezine-pulmonary tuberculosis. Ukuxhathisa kwe-rifampicin kubangelwa kakhulu kukuguquka kwe-rpoB gene. Nangona amayeza amatsha okulwa nesifo sephepha ephuma rhoqo, kwaye ukusebenza kakuhle kwezonyango kwezigulane ezine-pulmonary tuberculosis nako kuqhubekile nokuphucuka, kusekho ukunqongophala kwamayeza okulwa nesifo sephepha, kwaye imeko yokusetyenziswa kweziyobisi ezingenangqondo kwiklinikhi iphezulu kakhulu. Ngokucacileyo, isifo sephepha iMycobacterium kwizigulane ezine-pulmonary tuberculosis asinakubulawa ngokupheleleyo ngexesha elifanelekileyo, nto leyo ekugqibeleni ekhokelela kumanqanaba ahlukeneyo okuxhathisa amayeza emzimbeni wesigulane, yandise ixesha lesifo, kwaye yonyusa umngcipheko wokufa kwesigulane. Le khithi ifanelekile ekuxilongweni okuncediswayo kosulelo lwesifo sephepha iMycobacterium kunye nokufunyanwa kwe-rifampicin resistance gene, eluncedo ekuqondeni ukuxhathisa kwamayeza kwi-mycobacterium tuberculosis eyosulelwe zizigulane, kunye nokubonelela ngeendlela ezincedisayo zesikhokelo samayeza eklinikhi.
I-Epidemiology
| Igama elijoliswe kulo | Intatheli | Umcimi-mlilo | ||
| I-Reaction BufferA | I-Reaction BufferB | I-Reaction BufferC | ||
| rpoB 507-514 | rpoB 513-520 | IS6110 | I-FAM | Akukho nanye |
| rpoB 520-527 | rpoB 527-533 | / | CY5 | Akukho nanye |
| / | / | Ulawulo lwangaphakathi | I-HEX(VIC) | Akukho nanye |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-sputum |
| CV | 5% |
| I-LoD | uhlobo lwasendle olumelana ne-rifampicin: 2x103iintsholongwane/mL i-homozygous mutant: 2x103iintsholongwane/mL |
| Ukuchaneka | Ifumanisa isifo sephepha se-mycobacterium esikwindalo kunye neendawo zokuguquka kwezinye iijini ezichasene namayeza ezifana ne-katG 315G>C\A, InhA-15C>T, iziphumo zovavanyo azibonisi kuxhathisa kwi-rifampicin, oko kuthetha ukuba akukho ntshukumo idibeneyo. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) |
Ukuhamba komsebenzi
