Impilo yesini ingaphezulu kakhulu kokubakho okanye ukungabikho kweentsholongwane.

Indawo engqongileyo yesini yinkqubo yendalo eguquguqukayo ephenjelelwa kukunxibelelana phakathi kweendawo ezinobungozi, iintsholongwane ezinamathuba, izinto ezibangela ukugula, kunye neendlela zokuxhathisa iintsholongwane. Utshintsho ngaphakathi kwesi sixhobo sendalo lunokuba negalelo kwiimeko ezifana ne-bacterial vaginosis (BV), usulelo lwesini oluphindaphindayo, kunye nemingeni kulawulo lweklinikhi.

Kangangamashumi eminyaka, uvavanyo lwempilo yesini luxhomekeke kwiindlela eziqhelekileyo ezifana ne-microscopy, uvavanyo lwe-pH yesini, kunye ne-Amsel's criteria critic criteria. Ezi ndlela zihlala zizixhobo ezibalulekileyo kwizonyango; nangona kunjalo, zinokuba nemida xa kuvavanywa utshintsho oluntsonkothileyo lweentsholongwane, iiprofayili zeentsholongwane ezixutyiweyo, kunye nolwazi olunxulumene nokuxhathisa iintsholongwane.

Njengoko ulwazi lwesayensi malunga ne-vaginal microbiome luqhubeka luhambela phambili, izikhokelo zamazwe ngamazwe kunye neengcebiso zeklinikhi zigxininisa ngakumbi ukubaluleka kokuqwalasela ibhalansi yeentsholongwane kunye nokufunyanwa kwezifo.

Ukuxhasa le meko yokuxilonga etshintshayo,I-Macro kunye ne-Micro-Test zazisa i-Women's Health Advanced Nucleic Acid Detection Kit, isisombululo esibanzi seemolekyuli esenzelwe ukufumanisaIithagethi ezingama-20 ezifanelekileyo kwiklinikhi kuvavanyo olunye, enika ulwazi malunga neentsholongwane ezinxulumene ne-vaginal microbiota, iintsholongwane ezinobungozi, kunye nee-antimicrobial resistance (AMR).

I. Ukusuka ekuFunyanweni kwePathogen ukuya kuVavanyo olupheleleyo lweNdalo yesini

Imbono Entsha Ngempilo Yabasetyhini Yangasese

I-bacterial vaginosis (BV) yenye yezona zinto zixhaphakileyo zokukhupha ulwelo olungaqhelekanga kwisini phakathi kwabasetyhini abakwiminyaka yokuzala, kwaye ukuxhaphaka kwale ntsholongwane kuqikelelwa ukuba kumalunga23–29%.

Ngokutsho kweWorld Health Organisation (WHO), i-BV inxulunyaniswa nokungalingani kwi-vaginal microbiota, apho utshintsho kwimo yoluntu lweentsholongwane lunokuphazamisa imeko eqhelekileyo ye-vaginal.

I-American College of Obstetricians and Gynecologists (ACOG) ichaza i-BV ngokufanayo njengotshintsho kuluntu oluqhelekileyo lwebhaktheriya yesini endaweni yokubakho nje kwento enye ebangela usulelo.

Olu lwazi luye lwandisa umbono weklinikhi ngempilo yesini. Nangona ukuchonga iintsholongwane ezifanelekileyo kusabalulekile, ukuvavanya imeko-bume ebanzi yeentsholongwane kunokubonelela ngolwazi olongezelelweyo lokuqonda imeko yempilo yesini.

Iindlela ezikhoyo ngoku ziya zisiya phambili ngakumbi ngaphaya kokufunyanwa kwesifo esijolise kwinto enye ukuya kuvavanyo olupheleleyo oluqwalasela oku kulandelayo:

• · Utshintsho kwibhalansi yeentsholongwane zesini
• · Ubukho beentsholongwane ezincinci ezifanelekileyo kwiklinikhi
• · Iindlela ezinokubakho zokuxhathisa iintsholongwane

Olu tshintsho lubonisa ukuqondwa okukhulayo kokuba impilo yesini ichaphazeleka yiyo yonke inkqubo ye-microbial endaweni yesiphumo esinye sokufumanisa.

II. Kutheni Uvavanyo Olupheleleyo Lweemolekyuli Lubalulekile

Iimeko zempilo yesini zingabandakanya izinto ezininzi ezinegalelo. Iimpawu ezifanayo zeklinikhi zinokuvela kwiiprofayili ezahlukeneyo zeentsholongwane, kwaye utshintsho lweentsholongwane lunokuchaphazela ukuphinda kwesifo kunye nokuqwalaselwa konyango.

Indlela ebanzi yemolekyuli inokunceda iilabhoratri zifumane ulwazi oluthe kratya ngokudibanisa imilinganiselo emininzi yokuxilonga ngaphakathi komsebenzi omnye.

IIkhithi yokuFumanisa i-Nucleic Acid yeMpilo yabasetyhini yeMacro kunye neMicro-Testidibanisa:

1. Uvavanyo lweVaginal Microbiota

Inika ulwazi olunxulumene neempawu zoluntu lweentsholongwane kwaye ixhasa uvavanyo lokulinganisela kweentsholongwane zesitho sangasese sowesifazane.

2. Ukufunyanwa kwePathogen ngamathuba

Ivumela ukubonwa ngaxeshanye kweentsholongwane ezinxulumene nezonyango ezinxulumene neemeko zempilo yesini.

3. Uhlalutyo lweMakethi yokuchasana neentsholongwane

Ibonelela ngolwazi olongezelelweyo lweemolekyuli kwiijini ezinxulumene nokuxhathisa ukuxhasa ulawulo lweentsholongwane.

Ngokudibanisa ezi zakhono, isisombululo sinceda ukuvala umsantsa phakathi kovavanyo oluqhelekileyo kunye nemfuno ekhulayo yokuqonda okubanzi kweemolekyuli.

III. Ukuxhasa Ulawulo Lwe-Antimicrobial Ngokusebenzisa Ulwazi Lweemolekyuli

Ukumelana neentsholongwane (i-AMR) kuye kwaba yingxaki enkulu kwimpilo yoluntu kwihlabathi liphela.

Ngokutsho kwe-World Health Organisation (WHO), ukusetyenziswa ngokufanelekileyo kwee-antimicrobial kunye nokuphuculwa kwamandla okuxilonga zizinto ezibalulekileyo ekusombululeni imingeni ye-AMR.

Kwimpilo yabasetyhini, ukuqonda iindlela ezinokubakho zokuxhathisa kunokubonelela ngolwazi olongezelelweyo xa kuvavanywa iindlela zonyango kunye nokulawula iimeko eziphindaphindayo.

I-Macro & Micro-Test Women's Health Advanced Nucleic Acid Detection Kit ibandakanya ukufunyanwa kweempawu zokuxhathisa ezifanelekileyo ngokwezonyango:

• · I-TetM— enxulumene neendlela zokuxhathisa i-tetracycline
• · i-ermB— enxulunyaniswa neendlela zokuxhathisa ze-macrolide-lincosamide-streptogramin
• · i-mefA— enxulumene neendlela zokuxhathisa i-macrolide
• · i-bLATEM— enxulumene neendlela zokuxhathisa i-beta-lactam

Ngokudibanisa uhlalutyo lwe-AMR marker kunye nokufunyanwa kweentsholongwane, esi sisombululo sixhasa uvavanyo olupheleleyo lweemolekyuli kwaye sinegalelo kwizicwangciso zolawulo lweentsholongwane ezinolwazi.

IV. Ikhithi yokuFumanisa i-Nucleic Acid yeMpilo yabasetyhini yeMacro kunye neMicro-Test

Uvavanyo olunye. Ulwazi olupheleleyo ngeMpilo yesini.

Yenzelwe ukuxilongwa kweemolekyuli zanamhlanje, iWomen's Health Advanced Nucleic Acid Detection Kit ibonelela:

Ukufunyanwa okubanzi kwe-20-Target

Ukufunyanwa ngaxeshanye kweentsholongwane ezinxulumene ne-vaginal microbiota, iintsholongwane ezinobungozi, kunye neempawu zokumelana neentsholongwane.

Uvavanyo lweMicrobiome kunye nePathogen oluDibeneyo

Ixhasa uvavanyo lwebhalansi yeentsholongwane ngelixa ichonga iintsholongwane ezifanelekileyo kwiklinikhi.

Iimbono zeMolekyuli ezinxulumene ne-AMR

Kubandakanya ukufunyanwa kweempawu zokuxhathisa zeTetM, ermB, mefA, kunye ne-bLATEM.

Uvakalelo oluphezulu lohlalutyo

Umda wokufunyanwa (i-LOD):Iikopi ezingama-800/mL

Ukuhambelana kwePlatform eguquguqukayo

Iyahambelana namaqonga e-PCR aqhelekileyo ngexesha langempela kunye nesampulu yokuya kwisiphumo ezenzekelayo ngokupheleleyoUvavanyo lweMacro kunye nolweMicro Eudemon™ AIO800inkqubo yokuxilonga iimolekyuli.

Uhlobo lweSampuli oluLungileyo

Isampulu esebenzayo:Iswabhu yomlomo wesibeleko/yesifazane

Indawo yokugcina izinto ezinzileyo kunye nokuthuthwa

Imeko yokugcina:2–25°C
Beka ubomi kwishelufa:Iinyanga ezili-12

V. Izikhokelo zokuVala kunye nokuSebenza kwezonyango

Njengoko i-BV ichaphazela inani elikhulu labasetyhini abakwiminyaka yokuzala kwihlabathi liphela, ukuphinda isifo sibuyele esiqhelweni kusengumngeni wezonyango, kwaye ukumelana neentsholongwane kuqhubeka nokuchaphazela ulawulo lwezifo ezosulelayo, iindlela ezibanzi zokuxilonga ziya zisiba zibaluleke ngakumbi.

I-Macro & Micro-Test Women's Health Advanced Nucleic Acid Detection Kit imele isizukulwana esitsha sovavanyo lweemolekyuli oluyilelwe ukuhambelana nokuqonda okuguqukayo kwempilo yesini.

Ngokudibanisa:Uvavanyo lweMicrobiome + Ukufunyanwa kwePathogen + Uhlalutyo lwe-AMR, Isisombululo sinceda iilabhoratri kunye neengcali zezempilo ukuba ziqonde ngokupheleleyo impilo yesini, zixhasa uvavanyo lokuxilongwa olunolwazi oluthe kratya kunye nokwenza izigqibo zeklinikhi.

Impilo yabasetyhini ayichazwa yintsholongwane enye.

Ibunjwa bubudlelwane obunzima phakathi kweendawo ezihlala iintsholongwane, iimeko zonyango, kunye nempilo yomntu ngamnye.

Ngaphaya kokosuleleka. Ukuqonda ngokupheleleyo impilo yabasetyhini kwisini.