Rhoqo ngonyaka ngomhla we-17 kuTshazimpuzi lusuku loMhlaza lweHlabathi.
01 Isishwankathelo sezehlo zoMhlaza kwiHlabathi
Kwiminyaka yakutshanje, ngokunyuka okuqhubekayo kobomi babantu kunye noxinzelelo lwengqondo, iziganeko zamathumba nazo ziyanda unyaka nonyaka.
Amathumba amabi (umhlaza) abe yenye yeengxaki ezinkulu zempilo yoluntu ezibeka esichengeni impilo yabantu baseTshayina.Ngokutsho kwedatha yezibalo zamva nje, ukufa kwamathumba anobungozi kubangela i-23.91% yazo zonke izizathu zokufa phakathi kwabahlali, kwaye iziganeko kunye nokufa kwezicubu ezinobungozi ziye zaqhubeka zikhula kwiminyaka elishumi edlulileyo.Kodwa umhlaza awuthethi "isigwebo sokufa."I-World Health Organization yachaza ngokucacileyo ukuba nje ukuba ifunyenwe kwangoko, i-60% -90% ye-cancer inokunyangeka!Isinye kwisithathu semihlaza siyathinteleka, isinye kwisithathu somhlaza siyanyangeka, kwaye isinye kwisithathu somhlaza sinokunyangwa ukuze kwandiswe ubomi.
02 Yintoni ithumba
Ithumba libhekisela kumzimba omtsha owenziwe ngokusasazeka kweeseli zethishu zendawo phantsi kwesenzo sezinto ezahlukeneyo ze-tumor.Uphononongo lufumene ukuba iiseli ze-tumor ziye zitshintshe kwi-metabolic eyahlukileyo kwiiseli eziqhelekileyo.Kwangaxeshanye, iiseli zethumba ziyakwazi ukuziqhelanisa neenguqu kwimeko yemetabolism ngokutshintsha phakathi kwe-glycolysis kunye ne-oxidative phosphorylation.
03 Unyango lweCancer oluzimeleyo
Unyango lomhlaza womntu ngamnye lusekwe kulwazi lokuxilonga lwemfuza ekujoliswe kuyo isifo kunye neziphumo zophando lwezonyango olusekwe kubungqina.Inika isiseko sokuba izigulane zifumane isicwangciso sonyango esichanekileyo, esiye saba yindlela yophuhliso lwangoku lwezonyango.Izifundo zeklinikhi ziqinisekisile ukuba ngokufumanisa ukuguqulwa kofuzo lwee-biomarkers, i-gene SNP typing, i-gene kunye nesimo sayo seprotheyini ye-protein kwiisampulu ze-biological zezigulane ze-tumor ukuqikelela ukusebenza kwechiza kunye nokuvavanya i-prognosis, kunye nokukhokela unyango olulodwa lwekliniki, lunokuphucula ukusebenza kunye nokunciphisa okubi. reactions , ukukhuthaza ukusetyenziswa okunengqiqo kwezibonelelo zonyango.
Uvavanyo lwemolekyuli yomhlaza lunokwahlulwa lube ziindidi ezi-3 eziphambili: ukuxilonga, ufuzo, kunye nonyango.Uvavanyo lonyango lusembindini wento ebizwa ngokuba "yi-pathology yonyango" okanye iyeza elenzelwe umntu, kwaye ngakumbi nangakumbi amajoni omzimba kunye nezithinteli zemolekyuli ezincinci ezinokujolisa kwiijini eziphambili zethumba kunye neendlela zokubonisa zingasetyenziswa kunyango lwamathumba.
Unyango olujoliswe kwimolekyuli yamathumba lujolise kwiimolekyuli ezimakishayo zeeseli zethumba kwaye zingenelele kwinkqubo yeeseli ezinomhlaza.Isiphumo salo ikakhulu kwiiseli zethumba, kodwa zinefuthe elincinci kwiiseli eziqhelekileyo.I-Tumor growth factor receptors, i-signal transduction molecules, i-cell cycle proteins, i-apoptosis regulators, i-proteolytic enzymes, i-vascular endothelial growth factor, njl.Ngomhla wama-28 kuDisemba, ngo-2020, "iMiqathango yoLawulo lweSicelo seKlinikhi ye-Antineoplastic Drugs (Trial)" ekhutshwe yiKomishoni yezeMpilo yeSizwe kunye neyoNyango yabonisa ngokucacileyo ukuba: Kumachiza aneethagethi zemfuza ezicacileyo, umgaqo wokusetyenziswa kwawo kufuneka ulandelwe emva kokuba. uvavanyo lwemfuza ekujoliswe kuyo.
04 Uvavanyo lwemfuzo olujoliswe kwithumba
Kukho iintlobo ezininzi zokuguqulwa kofuzo kumathumba, kwaye iintlobo ezahlukeneyo zokuguqulwa kofuzo zisebenzisa iziyobisi ezijoliswe kuzo.Kuphela ngokucacisa uhlobo lokuguqulwa kofuzo kunye nokukhetha ngokuchanekileyo unyango lweyeza elijoliswe kuyo izigulane zinokuzuza.Iindlela zokubona iimolekyuli zisetyenziselwe ukufumanisa ukuhluka kweejini ezinxulumene neziyobisi ezijoliswe ngokuqhelekileyo kwiithumba.Ngokuhlalutya impembelelo yokwahluka kofuzo ekusebenzeni kwechiza, sinokunceda oogqirha baphuhlise esona sicwangciso sifanelekileyo sonyango lomntu ngamnye.
05 Isisombululo
IMacro kunye neMicro-Test iphuhlise uthotho lweekhithi zokubona ukuchongwa kwe-tumor gene, ebonelela ngesisombululo esipheleleyo sonyango olujoliswe kwithumba.
I-EGFR yoMntu yeGene 29 yeKhithi yokubona iiNguqu (Fluorescence PCR)
Le khithi isetyenziselwa kwi-vitro ngokusemgangathweni ukufumanisa utshintsho oluqhelekileyo kwi-exons 18-21 yejini ye-EGFR kwiisampulu ezivela kwizigulane ezinomhlaza wemiphunga yeseli yabantu engeyincinane.
1. Inkqubo yazisa ulawulo lomgangatho wereferensi yangaphakathi, enokubeka iliso ngokubanzi inkqubo yovavanyo kwaye iqinisekise umgangatho wovavanyo.
2. Uvakalelo oluphezulu: Ukufunyanwa kwesisombululo se-nucleic acid reaction kunokuqaphela ngokuzinzileyo izinga lokuguqulwa kwe-1% phantsi kwemvelaphi ye-3ng / μL yohlobo lwasendle.
3. Ukucaciswa okuphezulu: Akukho mpendulo kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo.
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KRAS 8 Ikhithi yokuFumana iiNguqu (Fluorescence PCR)
Le khithi yenzelwe ukufumanisa umgangatho we-in vitro we-8 utshintsho kwi-codons 12 kunye ne-13 ye-K-ras gene kwi-DNA ekhutshiweyo kumacandelo e-pathological yabantu afakwe kwiparafini.
1. Inkqubo yazisa ulawulo lomgangatho wereferensi yangaphakathi, enokubeka iliso ngokubanzi inkqubo yovavanyo kwaye iqinisekise umgangatho wovavanyo.
2. Uvakalelo oluphezulu: Ukufunyanwa kwesisombululo se-nucleic acid reaction kunokuqaphela ngokuzinzileyo izinga lokuguqulwa kwe-1% phantsi kwemvelaphi ye-3ng / μL yohlobo lwasendle.
3. Ukucaciswa okuphezulu: Akukho mpendulo kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo.
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IKit ye-EML4-ALK Fusion yeGene yoMntu yokuFumana (i-Fluorescence PCR)
Le khithi isetyenziselwa ukubona ngokusemgangathweni iintlobo ezili-12 zoguqulo lwe-EML4-ALK yofuzo lwemfuza kwiisampulu zezigulane zomhlaza wemiphunga weseli yabantu ezingancinci kwi-vitro.
1. Inkqubo yazisa ulawulo lomgangatho wereferensi yangaphakathi, enokubeka iliso ngokubanzi inkqubo yovavanyo kwaye iqinisekise umgangatho wovavanyo.
2. Uvakalelo oluphezulu: Le khithi inokubona ukuguqulwa kwe-fusion engaphantsi kweekopi ze-20.
3. Ukucaciswa okuphezulu: Akukho mpendulo kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo.
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I-ROS1 ye-Fusion Gene Mutation yeKit yoMntu yokuFumana (i-Fluorescence PCR)
Le khithi isetyenziselwa ukufunyaniswa komgangatho we-vitro weentlobo ezili-14 ze-ROS1 yokuguqulwa kofuzo lwe-fusion kwiisampulu zomhlaza wemiphunga weseli ezingezizo ezincinci.
1. Inkqubo yazisa ulawulo lomgangatho wereferensi yangaphakathi, enokubeka iliso ngokubanzi inkqubo yovavanyo kwaye iqinisekise umgangatho wovavanyo.
2. Uvakalelo oluphezulu: Le khithi inokubona ukuguqulwa kwe-fusion engaphantsi kweekopi ze-20.
3. Ukucaciswa okuphezulu: Akukho mpendulo kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo.
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IKit ye-BRAF yoMntu ye-V600E yokuFundisa iNguqu (i-Fluorescence PCR)
Le khithi yovavanyo isetyenziselwa ukukhangela ngokomgangatho ukuguqulwa kofuzo lwe-BRAF ye-V600E kwiisampulu zeparafini ezifakwe kwi-melanoma yomntu, umhlaza we-colorectal, umhlaza wedlala kunye nomhlaza wemiphunga kwi-vitro.
1. Inkqubo yazisa ulawulo lomgangatho wereferensi yangaphakathi, enokubeka iliso ngokubanzi inkqubo yovavanyo kwaye iqinisekise umgangatho wovavanyo.
2. Uvakalelo oluphezulu: Ukufunyanwa kwesisombululo se-nucleic acid reaction kunokuqaphela ngokuzinzileyo izinga lokuguqulwa kwe-1% phantsi kwemvelaphi ye-3ng / μL yohlobo lwasendle.
3. Ukucaciswa okuphezulu: Akukho mpendulo kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo.
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| Inombolo Yekhathalogu | Igama lemveliso | Inkcazo |
| HWTS-TM012A/B | IKit ye-EGFR yoMntu yeGene 29 yoFundo lweNguqu (Fluorescence PCR) | Iimvavanyo ezili-16/ikhithi, iimvavanyo ezingama-32/ikhithi |
| HWTS-TM014A/B | IKRAS 8 yeKit yokuFumana iiNguqu (Fluorescence PCR) | Iimvavanyo ezingama-24/ikhithi, iimvavanyo ezingama-48/ikhithi |
| HWTS-TM006A/B | IKit ye-EML4-ALK Fusion yeGene yoMntu yokuFumana (i-Fluorescence PCR) | Iimvavanyo ezingama-20/ikhithi, iimvavanyo ezingama-50/ikhithi |
| HWTS-TM009A/B | I-ROS1 ye-Fusion Gene Mutation yeKit yoMntu yokuFumana (i-Fluorescence PCR) | Iimvavanyo ezingama-20/ikhithi, iimvavanyo ezingama-50/ikhithi |
| HWTS-TM007A/B | IKit ye-BRAF yoMntu ye-V600E yokuFundisa iNguqu (i-Fluorescence PCR) | Iimvavanyo ezingama-24/ikhithi, iimvavanyo ezingama-48/ikhithi |
| HWTS-GE010A | I-BCR-ABL yoMntu iFusion Gene Mutation Kit (Fluorescence PCR) | Iimvavanyo ezingama-24/ikhithi |
Ixesha lokuposa: Apr-17-2023