Uvavanyo lwe-HPV Genotyping njengee-Biomarkers zokuxilonga umngcipheko womhlaza wesibeleko – Ngokusebenzisa i-HPV Genotyping Detection

Usulelo lwe-HPV luxhaphakile kubantu ababandakanyeka kwisondo, kodwa usulelo oluqhubekayo lukhula kuphela kwinani elincinci lamatyala. Ukuqhubeka kwe-HPV kubandakanya umngcipheko wokufumana izilonda zomlomo wesibeleko ngaphambi komhlaza, kwaye ekugqibeleni, umhlaza womlomo wesibeleko.

Ii-HPV azinakukhulai-In vitrongeendlela eziqhelekileyo, kunye notshintsho olukhulu lwendalo lwempendulo yomzimba emva kosulelo kuphazamisa ukusetyenziswa kovavanyo lwe-antibody ethile ye-HPV ekuxilongweni. Ngoko ke, ukuxilongwa kosulelo lwe-HPV kufezekiswa ngovavanyo lweemolekyuli, ikakhulu ngokufumanisa i-genomic HPV DNA.

Okwangoku, kukho iindlela ezahlukeneyo zokuchonga i-HPV zorhwebo. Ukukhethwa kwendlela efanelekileyo kuxhomekeke kwindlela ekucetywayo ukuyisebenzisa, oko kukuthi: i-epidemiology, uvavanyo lwe-vaccine, okanye izifundo zeklinikhi.

Kwiizifundo ze-epidemiological, iindlela zokuchonga i-HPV genotyping zivumela ukuba kubonwe uhlobo oluthile lokusasazeka kwesifo.
Ukuvavanya ugonyo, olu vavanyo lubonelela ngedatha malunga notshintsho ekuxhaphakeni kweentlobo ze-HPV ezingafakwanga kwiigonyo zangoku, kwaye lwenza kube lula ukulandelelwa kosulelo oluqhubekayo.
Kwizifundo zeklinikhi, izikhokelo zamazwe ngamazwe zangoku zicebisa ukusetyenziswa kovavanyo lwe-HPV genotyping phakathi kwabasetyhini abaneminyaka engama-30 nangaphezulu abane-cytology engalunganga kunye neziphumo ze-HR HPV positive, kwi-HPV-16 ekhethekileyo kunye ne-HPV-18. Ukufumanisa i-HPV kunye nokucalula ii-genotypes ezinomngcipheko ophezulu kunye nophantsi kabini okanye ngaphezulu ukufumana izigulane ezine-genotype efanayo yosulelo oluqhubekayo, okukhokelela kulawulo olungcono lweklinikhi.

Iikiti zokuchonga i-genotyping ye-HPV yeMacro kunye neMicro-Test:

• Iintlobo ezili-14 zeHPV (iGenotyping) Ikhithi yokuFumanisa iNucleic Acid (iFluorescence PCR)
• Ikhithi yokuFumanisa iNucleic Acid eyomileyo eqandisiweyo (i-PCR yeFluorescence) yeentlobo ezili-14 zeHPV (iGenotyping)
• Iintlobo ezingama-28 zeHPV (iGenotyping) Ikhithi yokuFumanisa (iFluorescence PCR)(iiHR-HPV ezili-18 +iiLR-HPV ezili-10)
• Isixhobo sokufumanisa ii-HPV eziyi-28 ezomisiweyo ngokuqandisa (iiGenotyping) (iFluorescence PCR)

Iimpawu eziphambili zemveliso:

• Ukufunyanwa ngaxeshanye kwee-genotypes ezininzi kwimpendulo enye;
• Ixesha elifutshane lokutshintsha i-PCR ukuze kwenziwe izigqibo zeklinikhi ngokukhawuleza;
• Iintlobo ezininzi zeesampulu (umchamo/iswab) ukuze kuhlolwe usulelo lwe-HPV ngendlela ekhululekileyo nefikelelekayo;
• Ulawulo lwangaphakathi oluBini luthintela iziphumo ezingezizo ezilungileyo kwaye luqinisekisa ukuthembeka kovavanyo;
• Iinguqulelo zolwelo kunye ne-lyophilized kwiinketho zabathengi;
• Ukuhambelana neenkqubo ezininzi ze-PCR ukuze kube lula ukuziqhelanisa neelebhu.