UVavanyo lwe-HPV Genotyping njenge-Diagnostic Biomarkers ye-Cervical Cancer Risk-Kwizicelo zoFundo lwe-HPV Genotyping

Usulelo lwe-HPV luxhaphakile kubantu ababelana ngesondo, kodwa usulelo oluzingileyo lukhula kuphela kwinani elincinci lamatyala. Ukuzingisa kwe-HPV kubandakanya umngcipheko wokuphuhlisa izilonda zomlomo wesibeleko kwaye, ekugqibeleni, umhlaza womlomo wesibeleko.

Ii-HPVs azinakukhuliswai-In vitrongeendlela eziqhelekileyo, kunye nokwahluka kwendalo okubanzi kwempendulo yomzimba yokuzikhusela emva kokuba usulelo luphazamisa ukusetyenziswa kovavanyo lwe-antibody olukhethekileyo lwe-HPV ekuxilongeni. Uxilongo losulelo lwe-HPV, ke ngoko, luphunyezwa ngovavanyo lwemolekyuli, ikakhulu ngokufunyaniswa kwe-genomic HPV DNA.

Okwangoku, kukho iindlela ezahlukeneyo zokurhweba zeHPV genotyping. Ukukhethwa kweyona ifaneleke ngakumbi ixhomekeke kusetyenziso olucetywayo, oko kukuthi: i-epidemiology, uvavanyo lwesitofu sokugonya, okanye izifundo zonyango.

Kwizifundo ze-epidemiological, iindlela ze-HPV genotyping zivumela ukuzotywa kohlobo oluthile lokuxhaphaka.
Kuphononongo lwesitofu sokugonya, olu vavanyo lubonelela ngedatha ngokumalunga notshintsho kuxhaphaka lweentlobo ze-HPV ezingaqukwanga kwizitofu zokugonya zangoku, kwaye ziququzelele ukulandelelwa kosulelo oluzingileyo.
Kwizifundo zeklinikhi, izikhokelo zangoku zamazwe ngamazwe zincoma ukusetyenziswa kovavanyo lwe-HPV genotyping phakathi kwabasetyhini abaneminyaka engama-30 ubudala nangaphezulu abane-cytology engathandekiyo kunye neziphumo ezilungileyo ze-HR HPV, kwi-HPV-16 ekhethekileyo kunye ne-HPV-18. Ukufumanisa i-HPV kunye nokucalula i-genotypes ephezulu kunye nephantsi kwengozi kabini okanye ngaphezulu ukufumana izigulane ezine-genotype efanayo yezifo eziqhubekayo, ezikhokelela kulawulo olungcono lweklinikhi.

Iikhithi ze-HPV zokuchwetheza ze-Macro kunye noVavanyo oluncinci:

• Iindidi ezili-14 zeHPV (Genotyping) iNucleic Acid Detection Kit (Fluorescence PCR)
• Iintlobo zeHPV ezili-14 ezikhenkcezisiweyo (Genotyping) iNucleic Acid Detection Kit(Fluorescence PCR)
• Iintlobo ezingama-28 ze-HPV (Genotyping) Ikhithi yokuFumana (i-Fluorescence PCR)(18 HR-HPVs +10 LR-HPVs)
• Iindidi ezingama-28 zeHPV ezomiswe ngumkhenkce( Genotyping) Ikhithi yokuFumana (i-Fluorescence PCR)

Iimpawu eziphambili zemveliso:

• Ukufunyanwa ngaxeshanye kwee-genotypes ezininzi kwimpendulo enye;
• Ixesha elifutshane lokuguqula i-PCR kwizigqibo zeklinikhi ezikhawulezayo;
• Iintlobo zeesampulu ezongezelelekileyo (umchamo/uswabhu) ukuze kujongwe ukukhululeka nokufikeleleka ngakumbi kosulelo lweHPV;
• Ulawulo lwangaphakathi oluBini luthintela ubuxoki kunye nokuqinisekisa ukuthembeka kovavanyo;
• Ulwelo kunye neenguqulelo ze-lyophilized kwiinketho zabathengi;
• Ukuhambelana neenkqubo ezininzi zePCR zokuziqhelanisa ngakumbi nelebhu.