I-Human Papillomavirus (Iintlobo ezingama-28) Uhlalutyo lwe-genotyping
Igama lemveliso
I-HWTS-CC013-I-Human Papillomavirus (Iintlobo ezingama-28) Ikhithi yokuFumanisa iGenotyping (i-Fluorescence PCR)
I-HWTS-CC016A-I-Human Papillomavirus eyomileyo eqandisiweyo (iintlobo ezingama-28) Ikhithi yokuFumanisa iGenotyping (i-Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zangaphambili zibonise ukuba usulelo oluqhubekayo kunye nosulelo oluninzi lwe-human papillomavirus yenye yezona zinto zibalulekileyo zomhlaza womlomo wesibeleko. Okwangoku, kusekho ukunqongophala kweendlela zonyango ezisebenzayo ze-HPV, ngoko ke ukufunyaniswa kwangoko kunye nokuthintela kwangoko i-HPV yomlomo wesibeleko zezona zinto zibalulekileyo zokuthintela umhlaza. Ukuseka indlela elula, ethe ngqo nekhawulezayo yokuxilonga i-etiological kubaluleke kakhulu ekuxilongweni kweklinikhi komhlaza womlomo wesibeleko.
Itshaneli
| I-buffer yempendulo | I-FAM | I-VIC/HEX | I-ROX | CY5 |
| I-HPV Genotyping Reaction Buffer 1 | 16 | 18 | / | Ulawulo lwangaphakathi |
| I-HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Ulawulo lwangaphakathi |
| I-HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| I-HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| I-HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| I-HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| I-HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| I-HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Swab yoMlomo, I-Swab yoBufazi, Umchamo |
| Ct | ≤28 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-300/mL |
| ukuchaneka | Zonke iziphumo azibi xa ikhithi isetyenziselwa ukubona iisampuli ezingezizo ezithile ezineempembelelo ezifanayo nayo, kuquka i-ureaplasma urealyticum, i-chlamydia trachomatis yendlela yokuzala, i-candida albicans, i-neisseria gonorrhoeae, i-trichomonas vaginalis, i-mold, i-gardnerella kunye nezinye iintlobo ze-HPV ezingagutyungelwanga yikhithi. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Iinkqubo ze-PCR zeXesha langempela ezingama-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Jonga iKit IFU ukuze ufumane iinkcukacha
Isisombululo 1 (Ukukhupha i-nucleic acid ngokuzenzekelayo)
Isisombululo sesi-2: (I-Macro kunye ne-Micro-Test Sample Release Reagent)
Ii-reagents ziyafuneka kodwa azibonelelwa
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), iMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwa
I-1.5mL yeetyhubhu ze-centrifuge ze-DNase/RNase-Free, ii-tips ze-DNase/RNase-Free, i-0.2mL yeetyhubhu ze-PCR, i-desktop centrifuge, kunye ne-desktop oscillation mixer.
