—— Uphando oluHlanganisiweyo oluqhutywa yiZhejiang CDC, Macro & Micro-Test, kunye neChina CDC Ipapashwe kwiFrontiers in Cellular and Infection Microbiology
Isishwankathelo soFundo
NgoMeyi 2026, iFrontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) yapapasha iphepha elikhokelwa yiZhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), kunye neqela le-bioinformatics elivela eBeijing Macro & Micro-Test Bio-Tech Co., Ltd. kunye neNational Institute for Communicable Disease Control and Prevention (China CDC) njengababhali abancedisayo. Olu phononongo lunesihloko esithi:
"Ukuchongwa kunye nohlalutyo lwe-phylogenetic lweentlobo ezisixhenxe ze-Brucella abortus eZhejiang, eTshayina."
Olu phononongo lubonisa uhlalutyo lokuqala olucwangcisiweyo, olusekelwe kwi-phylogenetic traceability lwe-Brucella abortus (B. abortus) kwiPhondo laseZhejiang, eTshayina. Iqela lihlalutye iintlobo ezisixhenxe ze-isolates eziqokelelwe ukususela ngo-2015 ukuya ku-2025 (ezine ezivela ebantwini kunye neentlobo ezintathu ezivela kwinkomo ezivela eJinhua, eQuzhou, naseNingbo). Ezi ziphumo zibonelela ngobungqina be-genomic malunga nemvelaphi kunye neendlela zokudlulisela olu "hlobo luphezulu olusemantla" kummandla ongaqhelekanga wesifo esimazantsi empuma yeTshayina.
Imvelaphi kunye nokubaluleka
I-Brucellosis sisifo sezilwanyana esibangelwa yibhaktheriya yohlobo lweBrucella. I-Brucella abortus ihlasela kakhulu iinkomo kodwa inokubangela izifo nakubantu. E-China, i-brucellosis ibonisa umahluko omkhulu ngokwendawo: uninzi lweziganeko zenzeka kumaphondo asemantla (umz., Inner Mongolia, Shanxi, Heilongjiang). Ngokwahlukileyo koko, amaphondo asemazantsi, kuquka neZhejiang, ebesoloko elawulwa yiBrucella melitensis, kwaye zimbalwa kakhulu iimeko ezixeliweyo ze-B. abortus. Oku kwahluka kwengingqi kwenza ukuba ukuchongwa kwemfuza kunye nokulandelela umthombo we-B. abortus eZhejiang kube yeyona nto iphambili kwimpilo yoluntu.
Iindlela kunye neZiphumo eziphambili
Iqela lophando lamkele icebo elinamacandelo amaninzi elidibanisa ibhayoloji yemolekyuli kunye ne-bioinformatics:
1.Ukuchongwa kwezifo kunye nokubhala okusisiseko
I-BCSP-31 gene PCR kunye ne-AMOS-PCR ziqinisekisile ukuba zonke ii-isolates ezisixhenxe zaziyi-B. abortus.
Uhlobo lwe-Multilocus sequence typing (MLST) olusekelwe kwiijini ezilithoba zokugcinwa kwendlu lubonise ukuba zonke ii-isolates zazikwi-sequence type ST2, nto leyo ebonisa ukuba i-genetic homogeneity ephezulu phakathi kweentlobo ze-B. abortus ezijikelezayo eZhejiang.
2.Ukuchazwa kwe-genome epheleleyo
Ulandelelwano lwe-genome epheleleyo lwenziwe kwiqonga le-Illumina NovaSeq. Uhlalutyo oluqhelekileyo lwe-nucleotide identity (ANI) lubonise ukuba ii-isolates zaseZhejiang zabelana ngokufana okufikelela kwi-99.99% nohlobo lwereferensi i-B. abortus 544.
Uhlalutyo lwe-Pan-genome lubonise inani labantu abagcinwe kakuhle: ii-genes eziphambili ezingama-3,084 zichongiwe, kunye nee-genes ze-shell ezili-10 kuphela, kwaye akukho zi-genes ze-core okanye ze-cloud ezifunyenweyo.
3.Iiprofayili zejini zokumelana neentsholongwane kunye nokulwa neentsholongwane
Izinto ezingama-68 ezinxulumene nobungozi beziqikelelwe, zigubungela iindlela zakudala ezifana ne-LPS biosynthesis, inkqubo yokukhupha i-T4SS, kunye nenkqubo yokulawula yamacandelo amabini ye-BvrR-BvrS. Okuphawulekayo kukuba, zonke ii-isolates zazingenazo ii-adhesin genes bmaA kunye ne-btaF. Uhlalutyo lwe-resistance gene lufumanise kuphela i-mprF gene kwi-CARD database, kungekho ezinye izinto ezibangela ukuxhathisa ezichongiweyo.
4. Ukwakhiwa ngokutsha kwe-phylogenetic kunye nokulandelela ukusasazeka kwayo
Uhlalutyo lweCore-genome single-nucleotide polymorphism (cgSNP) lubeke ii-isolates zaseZhejiang kwindawo ethile kumthi we-phylogenetic wehlabathi. Iziphumo zibonise ukuba iintlobo zeZhejiang zenza iqela le-monophyletic kunye neentlobo ezivela eRashiya, eMongolia, kunye namaphondo amaninzi aseMantla eTshayina (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Eli qela lahlulwe laba zii-sub clades ezintathu ezahlukeneyo (Clade 1-3), nto leyo ebonisa iziganeko ezininzi zokwazisa ezizimeleyo.
Izigqibo kunye neziphumo
Olu phononongo lubonelela ngedatha yokuqala ye-genomic echanekileyo kakhulu ye-B. abortus kwiPhondo laseZhejiang kwaye ivelisa izigqibo ezibalulekileyo ezininzi:
- Clear imvelaphi yemfuza– Iintlobo ze-B. abortus ezijikeleza eZhejiang zeze-ST2, zigcinwe kakuhle ngokwezakhi zofuzo, kwaye zimele umnombo oqhelekileyo we-brucellosis yeenkomo.
2. U-Eviumngcipheko wothumelo lwemimandla enqamlezileyo– Uhlalutyo lwe-phylogenetic aluxhasi ukubakho koluhlu oluzimeleyo oluhlala lukhona eZhejiang. Endaweni yoko, idatha ibonisa ngamandla ukuba ezi ntlobo zivela kumantla eTshayina kwaye zinokwabelana ngemvelaphi efanayo yokuzivelela kunye neentlobo ezivela eRashiya naseMongolia. Ukubakho kweentlobo ezintathu ezingaphantsi kuthetha iziganeko ezininzi ezahlukeneyo zokungeniswa.
3. Impembelelo kwimpilo yoluntu– Ezi ziphumo zigxininisa ixabiso lokujonga i-genomic ye-brucellosis nakwiindawo ezingezizo ezixhaphakileyo njengeZhejiang. Nangona inani lamatyala akhoyo ngoku liphantsi, izixhobo ezisombulula iingxaki ezifana ne-cgSNP zinokulandela ngokufanelekileyo umthombo wokuqhambuka kwezifo ezingenisiweyo kwaye zibonelele ngobungqina besayensi bokuphazamisa uthungelwano lokudluliselwa kwezifo ezinxulumene nokuthuthwa kwemfuyo phakathi kwamaphondo.
Lo msebenzi awugcini nje ngokuzalisa umsantsa wophando kwiPhondo laseZhejiang kodwa ukwabonelela ngedatha entsha yokubeka esweni izifo kunye novavanyo lomngcipheko we-brucellosis kummandla weYangtze River Delta.
Ulwazi lwePhepha:
UYang, Y., uShi, X., uChen, J., uWang, L., uWu, Z., uYao, W., … kunye noWu, B. (2026). Ukuchongwa kunye nohlalutyo lwe-phylogenetic lweentlobo ezisixhenxe ze-Brucella abortus eZhejiang, eTshayina. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Ixesha lokuthumela: Juni-10-2026