I-DNA yeMycobacterium Tuberculosis

Umfanekiso obonisiweyo we-DNA yeMycobacterium Tuberculosis

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa izigulane ezineempawu/iimpawu ezinxulumene nesifo sephepha ngaphakathi kwi-vitro okanye iqinisekiswe ngovavanyo lwe-X-ray losulelo lwesifo sephepha se-mycobacterium kunye neesampuli zesikhohlela zezigulana ezifuna ukuxilongwa okanye ukuxilongwa ngokwahlukileyo kosulelo lwesifo sephepha se-mycobacterium.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa i-HWTS-RT102-Nucleic Acid esekwe kwi-Enzymatic Probe Isothermal Amplification (EPIA) yesifo sephepha seMycobacterium

Isatifikethi

I-Epidemiology

I-Mycobacterium tubercle tubercle (i-Tubercle bacillus, i-TB) luhlobo lwebhaktiriya ye-aerobic ebophelelayo ene-acid-fast staining entle. Kukho i-pili kwi-TB kodwa akukho flagellum. Nangona i-TB ine-microcapsules kodwa ayenzi ii-spores. Udonga lweseli lwe-TB aluna-teichoic acid yebhaktiriya ene-gram-positive okanye i-lipopolysaccharide yebhaktiriya ene-gram-negative. I-Mycobacterium tuberculosis ebangela izifo ebantwini ngokubanzi yahlulwe ngohlobo lomntu, uhlobo lwenkomo, kunye nohlobo lwase-Afrika. I-pathogenicity ye-TB inokunxulunyaniswa nokudumba okubangelwa kukwanda kwebhaktiriya kwiiseli zezicubu, ubuthi bezinto zebhaktiriya kunye ne-metabolites, kunye nomonakalo womzimba kwiinxalenye zebhaktiriya. Izinto ezibangela izifo zinxulumene nee-capsules, ii-lipids kunye neeproteni. I-Mycobacterium tuberculosis inokuhlasela uluntu olusengozini ngokusebenzisa indlela yokuphefumla, indlela yokugaya ukutya okanye umonakalo wesikhumba, ibangele isifo sephepha kwiintlobo ngeentlobo zezicubu kunye namalungu, apho isifo sephepha esibangelwa yindlela yokuphefumla sesona sikhulu. Sivela kakhulu ebantwaneni, sineempawu ezifana nomkhuhlane ophantsi, ukubila ebusuku, kunye nenani elincinci le-hemoptysis. Usulelo lwesibini lubonakala kakhulu njengomkhuhlane ophantsi, ukubila ebusuku, i-hemoptysis kunye nezinye iimpawu; ukuqala okungapheliyo, ukuhlaselwa okukhawulezileyo. Isifo sephepha sesinye sezizathu ezilishumi eziphambili zokufa ehlabathini. Ngo-2018, malunga nezigidi ezili-10 zabantu emhlabeni bosulelwe sisifo sephepha iMycobacterium, malunga nezigidi ezili-1.6 zabantu bafa. I-China lilizwe elinomthwalo omkhulu wesifo sephepha, kwaye izinga lokwanda kwaso likwindawo yesibini kwihlabathi liphela.

Itshaneli

I-FAM	Isifo sephepha seMycobacterium
CY5	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	Ulwelo: ≤-18℃;
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	I-sputum
Tt	≤28
CV	≤10％
I-LoD	Ulwelo: Iikopi eziyi-1000/mL,
Ukuchaneka	Akukho kudibana kweentsholongwane nezinye iintsholongwane ze-mycobacteria kwi-non-Mycobacterium tuberculosis complex (umz. iMycobacterium kansas, iMycobacter surga, iMycobacterium marinum, njl.njl.) kunye nezinye izifo (umz. iStreptococcus pneumoniae, iHaemophilus influenzae, iEscherichia coli, njl.njl.).
Izixhobo Ezisebenzayo (Ulwelo)	Inkqubo yokuFumanisa i-Fluorescence Isothermal ye-Easy Amp Real-time (HWTS1600),Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time,Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.)
Izixhobo Ezisebenzayo (Ezilungisiweyo)	Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iShanghai Hongshi Medical Technology Co., Ltd.) I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumana ubushushu obuQhelekileyo beFluorescence yexesha langempela i-Easy Amp HWTS1600