Ikhithi ye-Fluorescent ye-RT-PCR yexesha lokwenyani yokubona i-SARS-CoV-2
Igama lemveliso
Ikhithi ye-HWTS-RT057A-Ixesha lokwenyani le-fluorescent RT-PCR yokubona i-SARS-CoV-2
Ikhithi ye-HWTS-RT057F-Mkhenkce-yomileyo yexesha lokwenyani ye-RT-PCR yokubhaqa i-SARS-CoV-2 -Ipakethe
Isatifikethi
CE
I-Epidemiology
Inoveli coronavirus (SARS-CoV-2) isasazeke kwinqanaba elikhulu kwihlabathi liphela.Kwinkqubo yokusasaza, utshintsho olutsha luhlala lusenzeka, lukhokelela kutshintsho olutsha.Le mveliso isetyenziselwa ukufumanisa okuncedisayo kunye nokwahlulwa kwamatyala anxulumene nosulelo emva kokusasazeka okukhulu kwe-Alpha, Beta, Gamma, Delta kunye ne-Omicron yokuguquguquka kweentlobo ukusukela ngoDisemba ka-2020.
Umjelo
| FAM | Uhlobo lwe-2019-nCoV ORF1ab |
| CY5 | 2019-nCoV N yejini |
| VIC(HEX) | ijini yesalathiso yangaphakathi |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
| I-Lyophilized: ≤30℃ ebumnyameni | |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 |
| I-Lyophilized: iinyanga ezili-12 | |
| Uhlobo loMfanekiso | i-nasopharyngeal swabs, i-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 300Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda-phinda kusebenze kunye ne-coronavirus yabantu i-SARS-CoV kunye nezinye iipathogens eziqhelekileyo. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ukutsalwa kwe-Acid yeNucleic okanye iKit yokuCoca(iNdlela yeMagnetic Beads)(HWTS-3001, HWTS-3004-32, HWTS-3004-48) evela kwiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ukhetho lwesi-2.
Isixhobo sokutsalela esicetyiswayo: QIAamp Viral RNA Mini Kit (52904), Viral RNA Extraction Kit (YDP315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd.
