IiPathogens zokuphefumla zidibeneyo

IiPathogens zokuphefumla ezidityanisiweyo Umfanekiso okhoyo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufunyaniswa komgangatho we-in vitro intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B, intsholongwane yokuphefumla i-syncytial virus, i-adenovirus, i-human rhinovirus kunye ne-mycoplasma pneumoniae nucleic acids kwi-swabs yomntu ye-nasopharyngeal kunye neesampuli ze-oropharyngeal swab.Iziphumo zovavanyo zingasetyenziselwa uncedo ekuxilongeni usulelo lwe-pathogen yokuphefumula, kunye nokubonelela ngesiseko esincedisayo sokuxilongwa kwe-molecular yokuxilongwa kunye nokunyangwa kwezifo zokuphefumula.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT050-Iintlobo eziNtathu zePathogen yeNucleic Acid yokuphefumla(Fluorescence PCR)

I-Epidemiology

Umkhuhlane, owaziwa ngokuba 'ngumkhuhlane', sisifo esosulelayo esinamandla sokuphefumla esibangelwa yintsholongwane yomkhuhlane, neyosulela kakhulu kwaye isasazeka kakhulu ngokukhohlela kunye nokuthimla.

Intsholongwane yokuphefumla ye-syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae.

I-Human adenovirus (HAdV) yintsholongwane ye-DNA ephindwe kabini ngaphandle kwemvulophu.Ubuncinci i-90 genotypes ifunyenwe, enokuthi ihlulwe ibe yi-7 subgenera AG.

I-Human rhinovirus (HRV) lilungu losapho lwe-Picornaviridae kunye nohlobo lwe-Enterovirus.

I-Mycoplasma pneumoniae (MP) yintsholongwane ye-pathogenic ephakathi kwebhaktheriya kunye neentsholongwane ngokobukhulu.

Umjelo

Umjelo	PCR-Mix A	PCR-Mix B
FAM Channel	IFV A	I-HAdV
VIC/HEX Channel	HRV	IFV B
Ijelo le-CY5	RSV	MP
Ijelo le-ROX	Ulawulo lwangaphakathi	Ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino	-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	I-Oropharyngeal swab
Ct	≤35
LoD	500Iikopi/mL
Ukuchaza ngokuthe ngqo	1.Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo emnqamlezweni phakathi kwekhithi kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, iintlobo zentsholongwane ye-Parainfluenza 1, 2, kunye 3, Chlamydia pneumoniae, metapneumovirus yabantu, Enterovirus A, B, C, D, Epstein-Barr virus, intsholongwane yemasisi, cytomegalovirus yabantu, Rotavirus, Norovirus, Mumps virus, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influlococcus, aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans kunye ne-nucleic acids yabantu. 2.Ikhono lokuchasana nokuphazamiseka: i-Mucin (60mg / mL), i-10% (v / v) igazi lomntu, i-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (kunye ne-preservatives) (20mg / mL), i-beclomethasone ( 20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), kunye tobramycin (0.6mg/mL) zikhethwe kuvavanyo lokuphazamiseka, kwaye iziphumo zibonise ukuba izinto eziphazamisayo kwezi zigxininiso zingasentla azikho ukusabela kokuphazamiseka kwiziphumo zovavanyo lwe-pathogens.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, iBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani