Xinjiang Hemorrhagic Fever Virus
Igama lemveliso
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-Xinjiang hemorrhagic fever yaqala yahlukaniswa negazi lezigulana ezinomkhuhlane we-hemorrhagic kwi-Tarim Basin, e-Xinjiang, e-China kunye namakhalane anzima abanjwe ekuhlaleni, kwaye yafumana igama layo. ukubonakaliswa Clinical ziquka umkhuhlane, intloko ebuhlungu, ukopha, ukothuka hypotensive, etc. utshintsho olusisiseko pathological kwesi sifo inkqubo capillary dilatation, ukuxinana, ukwanda permeability kunye fragility, okubangela amanqanaba ahlukeneyo ukuxinana kunye ukopha eluswini kunye nenwebu kunye nezicubu zamalungu ahlukeneyo kuwo wonke umzimba, kunye nokuwohloka kunye nedlala kunye necrosis, i-adrenal gland kunye necrosis, idlala, i-adrenal gland, i-solid. I-edema efana nejeli kwi-retroperitoneum.
Umjelo
| FAM | Xinjiang Hemorrhagic Fever Virus |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | 1000Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye nezinye iisampulu zokuphefumla ezifana Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapnevirus A1/B2umotory A1/B2 syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njl kunye ne-DNA ye-genomic yabantu. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampulu ekhutshiweyo yi-200µL, kwaye umthamo we-elution ocetyiswayo yi-80µL.
I-reagent yokutsalwa ecetyiswayo: QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN kunye ne-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokuhambelana nemiyalelo yokusetyenziswa. Umthamo wesampulu ekhutshiweyo yi-140µL, kwaye umthamo we-elution ocetyiswayo yi-60µL.
