Intsholongwane yomkhuhlane we-Xinjiang Hemorrhagic Fever
Igama lemveliso
Ikhithi yokuFumana iNucleic Acid ye-HWTS-FE007B/C ye-Xinjiang Hemorrhagic Fever Virus (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-Xinjiang hemorrhagic fever yaqala ukubonwa egazini lezigulana ezine-hemorrhagic fever eTarim Basin, eXinjiang, eTshayina nakwii-hard ticks ezifunyenwe apha, yaza yafumana igama layo. Iimpawu zonyango ziquka umkhuhlane, intloko ebuhlungu, ukopha, ukothuka okubangelwa kuxinzelelo lwegazi, njl. Utshintsho olusisiseko lwesifo sesi sifo kukwanda kwe-capillary systemic, ukuxinana, ukwanda kokungena kwamanzi kunye nobuthathaka, okubangela amazinga ahlukeneyo okuxinana kunye nokopha eluswini nakwi-mucous membranes kunye nezicubu zamalungu ahlukeneyo emzimbeni wonke, kunye nokuwohloka kunye nokubola kwamalungu aqinileyo afana nesibindi, i-adrenal gland, i-pituitary gland, njl., kunye ne-jelly-like edema kwi-retroperitoneum.
Itshaneli
| I-FAM | Intsholongwane yomkhuhlane we-Xinjiang Hemorrhagic Fever |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | iserum entsha |
| Tt | ≤38 |
| CV | ＜5.0% |
| I-LoD | Iikopi ezili-1000/mL |
| Ukuchaneka | Akukho kudibana kweentsholongwane nezinye iisampuli zokuphefumla ezifana ne-Influenza A, i-Influenza B, i-Legionella pneumophila, i-Rickettsia Q fever, i-Chlamydia pneumoniae, i-Adenovirus, i-Respiratory Syncytial Virus, i-Parainfluenza 1, 2, 3, i-Coxsackie virus, i-Echo virus, i-Metapneumovirus A1/A2/B1/B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njl.njl. kunye ne-human genomic DNA. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wesampulu okhutshiweyo yi-200µL, kwaye umthamo wokucoca ocetyiswayo yi-80µL.
I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN kunye ne-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhupha kufuneka kwenziwe ngokuhambelana nemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-140µL, kwaye umthamo wokucoca ocetyiswayo yi-60µL.
