Iintlobo ezili-11 zePathogen yokuphefumla
Igama lemveliso
I-HWTS-RT162A Iindidi ezili-11 zeKiti yokuFumanisa i-Nucleic Acid yePathogen yokuPhefumla(I-PCR yeFluorescence)
Itshaneli
| 靶标 | Itshaneli | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | ILogo Simahla Akukho mlinganiselo Ipapashwe ngu- 核酸反应液D | |
| HI | Smet | MP | Umlenze | I-FAM |
| SP | PA | Bp | / | CY5 |
| I-KPN | I-ABA | I-Bpp | I-Cpn | I-ROX |
| 内参 | 内参 | 内参 | 内参 | I-VIC/HEX |
I-Epidemiology
Usulelo lwendlela yokuphefumla ludidi olubalulekileyo lwezifo ezisongela impilo yabantu, kwaye uphando lubonise ukuba uninzi losulelo lwendlela yokuphefumla lubangelwa ziintsholongwane okanye iintsholongwane ezihlasela umninimzi, nto leyo ekhokelela ekwandeni kobunzima besifo kwanokufa, ngoko ke ukucacisa intsholongwane kuvumela unyango olujoliswe kulo kwaye kuphucula izinga lokusinda kwesigulana.[1,2].
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-sputum |
| Ct | HI, SP, KPN, PA, ABA, Smet: Ct≤33I-BP, i-Bpp, i-MP, i-Cpn, umlenze: i-Ct≤38 |
| CV | <5.0% |
| I-LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasi-aeruginosa, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia kunye ne-Legionellai-pneumophila: 1000 CFU/mL； I-Bordetella pertussis kunye ne-Bacillus parapertussis: 500 CFU/mL; I-Mycoplasma pneumoniae kunye ne-Chlamydia pneumoniae: iikopi ezingama-200/mL. |
| Ukuchaneka | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo idibeneyo phakathi kwale kit kunye neCytomegalovirus, uhlobo lwe-Herpes simplex virus 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumophila, i-Moraxella catarrhalis, iintlobo ezincitshisiweyo ze-Mycobacterium tuberculosis, i-Neisseria meningitidis, i-Neisseria, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, IiCandida albicans, iRothia mucilaginosus, iStreptococcus oralis, iKlebsiella pneumoniae, iChlamydia psittaci, iCoxiella burnetii kunye nee-human genomic nucleic acids. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200µL yei-physiologicalFaka ityuwa kwi-precipitate elungisiweyo, kwaye amanyathelo alandelayo kufuneka enziwe ngokwemiyalelo. Umthamo wokucoca ocetyiswayo yi-100 µL.
