Intsholongwane yeHepatitis A
Igama lemveliso
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Intsholongwane yeHepatitis A (HAV) ngoyena nobangela we-acute viral hepatitis.Le ntsholongwane yintsholongwane ye-RNA enemisonto enye kwaye yeyeqela le-Hepadnavirus yosapho lwe-Picornaviridae.Intsholongwane yeHepatitis A, isasazwa ikakhulu yifecal-oral route, ekwaziyo ukumelana nobushushu, iiasidi, kunye nezinyibilikisi ezininzi eziphilayo, inokuphila ixesha elide kwi-shellfish, emanzini, emhlabeni, okanye kwintlenga yolwandle [1-3].Isasazwa ngokutya okanye amanzi angcolileyo, okanye isuka kumntu ukuya emntwini.Ukutya okunxulumene ne-HAV kubandakanya i-oyster kunye ne-clams, i-strawberries, i-raspberries, i-blueberries, i-date, imifuno enamagqabi aluhlaza, kunye ne-tomato eyomileyo[4-6].
Umjelo
| FAM | I-HAV nucleic acid |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9, iLyophilized: iinyanga ezili-12 |
| Uhlobo loMfanekiso | ISerum/isitulo |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezi-2/μL |
| Ukuchaza ngokuthe ngqo | Sebenzisa iikhithi ukuvavanya ezinye iintsholongwane hepatitis ezifana hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV kunye genome.Akukho cross-reactivity. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 yeXesha lokwenyani i-Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Iisampuli zeSerum
Ukhetho loku-1.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufuneka ikhutshwe ngokwemiyalelo.Umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho lwesi-2.
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwa yi-Tiangen Biotech (Beijing) Co., Ltd. Kufuneka ikhutshwe ngokwemiyalelo.Umthamo wesampulu ekhutshiweyo yi-140μL.Umthamo we-elution ocetyiswayo yi-60µL.
2.Iisampulu zesitulo
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufuneka ikhutshwe ngokwemiyalelo.Umthamo we-elution ocetyiswayo yi-80µL.
