Iintlobo ezili-14 zeePathogens zokuphefumla ezidityanisiweyo
Igama lemveliso
HWTS-RT159B Iindidi ezili-14 zeePathogens zokuphefumla eziDityanisiweyo zeNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Usulelo lwendlela yokuphefumla sesona sifo sixhaphakileyo ebantwini, esinokuthi senzeke nakwesiphi na isini, ubudala kunye nommandla. Ngomnye wezona zizathu ziphambili zokugula nokufa kwabantu kwihlabathi liphela[1]. Izifo eziqhelekileyo zokuphefumla zibandakanya inoveli coronavirus, intsholongwane yoMkhuhlane A, intsholongwane yoMkhuhlane B, intsholongwane yokuphefumla yesyncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, St.[2,3].
Umjelo
| Kulungile Isikhundla | Igama lesisombululo sempendulo | Iintsholongwane eziza kufunyanwa |
| 1 | Master Mix A | I-SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, ulawulo lwangaphakathi |
| 5 | Master Mix A | I-SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab, i-Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo phakathi kwale khithi kunye ne-Cytomegalovirus, i-Herpes simplex virus type 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella-zoster, i-legionellauxellauxellauxelles Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stekhomalcoccus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stekhomal Cococcus, bacterium Coccus, Coccus, Streptococcus salivarius, striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlaitimic nucleic acid burning human. |
| Izixhobo Ezisebenzayo | SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.)Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, iBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
Ukukhutshwa kwe-reagent ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isampuli ekhutshiweyo ye-L. Amanyathelo alandelayo kufuneka enziwe ngokwemiyalelo yokusetyenziswa kwesi sixhobo sokutsalwa. Umthamo we-lution ocetyiswayo ngu80µL.
