Iintlobo ezili-14 zeentsholongwane zokuphefumla ezidityanisiweyo
Igama lemveliso
I-HWTS-RT159B Iindidi ezili-14 zeePathogens zokuphefumla eziDibeneyo zeNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Ukosuleleka kwindlela yokuphefumla sesona sifo sixhaphakileyo ebantwini, esinokwenzeka nakweyiphi na isini, ubudala kunye nommandla. Sesinye sezona zizathu ziphambili zokugula nokufa kubantu kwihlabathi liphela.[1]Iintsholongwane eziqhelekileyo zokuphefumla ziquka i-coronavirus entsha, i-Influenza A virus, i-Influenza B virus, i-Respiratory syncytial virus, i-Adenovirus, i-human metapneumovirus, i-rhinovirus, i-Parainfluenza virus type I/II/III/IV, i-Bocavirus, i-Enterovirus, i-Coronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, kunye ne-Streptococcus pneumoniae, njl.njl.[2,3].
Itshaneli
| Indawo yoMthombo | Igama lesisombululo sempendulo | Iintsholongwane eziza kubonwa |
| 1 | Umxube weMaster A | I-SARS-CoV-2, i-IFV A, i-IFV B |
| 2 | Umxube weMaster B | I-Adv, i-hMPV, i-MP, i-Cpn |
| 3 | Umxube weMaster C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Umxube weMaster D | I-CoV, i-EV, i-SP, ulawulo lwangaphakathi |
| 5 | Umxube weMaster A | I-SARS-CoV-2, i-IFV A, i-IFV B |
| 6 | Umxube weMaster B | I-Adv, i-hMPV, i-MP, i-Cpn |
| 7 | Umxube weMaster C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Umxube weMaster D | I-CoV, i-EV, i-SP, ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | Iswabhu yomlomo, iswabhu yempumlo |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | 2Iikopi ze-00/mL |
| Ukuchaneka | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo idibeneyo phakathi kwale kit kunye neCytomegalovirus, uhlobo lwe-Herpes simplex virus 1, i-Varicella-zoster virus, i-Epstein-Barr virus, i-Bordetella pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumophila, i-Moraxella catarrhalis, iintlobo ezincitshisiweyo ze-Mycobacterium tuberculosis, i-Neisseria meningitidis, i-Neisseria, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas maltophilia, i-Burkholderia cepacia, i-Corynebacterium striatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis jiroveci, IiCandida albicans, iRothia mucilaginosus, iStreptococcus oralis, iKlebsiella pneumoniae, iChlamydia psittaci, iCoxiella burnetii kunye nee-human genomic nucleic acids. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.)Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 Real-Time, Inkqubo ye-PCR ye-BioRad CFX Opus 96 Real-Time |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampulu okhutshiweyo yi-200µL. Amanyathelo alandelayo kufuneka enziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wokucoca ocetyiswayo ngu80µL.
