I-Monkeypox Virus Nucleic Acid

Monkeypox Virus Nucleic Acid Umfanekiso obonakalayo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-monkeypox virus nucleic acid kulwelo lwerhashalala lomntu, i-nasopharyngeal swabs, i-swabs yomqala kunye neesampulu zeserum.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT071-Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-OT072-Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Isatifikethi

I-Epidemiology

I-Monkeypox (MP) sisifo esosulelayo esinamandla esibangelwa yiMonkeypox Virus (MPV).Ubukhulu becala esi sifo sisasazwa zizilwanyana, yaye abantu banokosulelwa ngokulunywa zizilwanyana ezosulelekileyo okanye ngokudibana ngokuthe ngqo negazi, ulwelo lomzimba nerhashalala lezilwanyana ezosulelekileyo.Intsholongwane inokosulela phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha elide, ngqo ubuso ngobuso okanye ngokudibana ngokuthe ngqo nolwelo lomzimba wesigulana okanye izinto ezingcolisekileyo.

Iimpawu zeklinikhi zosulelo lwemonkeypox ebantwini zifana nezo ze-smallpox, ngokubanzi emva kwexesha le-12-day incubation, ukubonakala komkhuhlane, intloko ebuhlungu, iintlungu zemisipha kunye nomqolo, i-lymph nodes eyandisiweyo, ukukhathala kunye nokungahambi kakuhle.I-rash ibonakala emva kweentsuku ezi-1-3 zomkhuhlane, ngokuqhelekileyo kuqala ebusweni, kodwa nakwezinye iindawo.Ikhosi yesifo ngokubanzi ithatha iiveki ezi-2-4, kwaye izinga lokufa li-1% -10%.I-Lymphadenopathy yenye yeeyantlukwano eziphambili phakathi kwesi sifo kunye ne-smallpox.

Umjelo

Umjelo	Inkawu	I-monkeypox kunye ne-Orthopox
FAM	Intsholongwane yemonkeypox MPV-1 gene	Intsholongwane ye-Orthopox i-universal type nucleic acid
VIC/HEX	Intsholongwane ye-monkeypox MPV-2 gene	Intsholongwane ye-monkeypox MPV-2 gene
ROX	/	Intsholongwane yemonkeypox MPV-1 gene
CY5	Ulawulo lwangaphakathi	Ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	I-Rash Fluid, i-Nasopharyngeal Swab, i-Throat Swab, iSerum
Ct	≤38
CV	≤5.0％
LoD	200Iikopi/mL
Ukuchaza ngokuthe ngqo	Akukho cross-reactivity kunye nentsholongwane yengqakaqha, Cowpox virus, Vaccinia virus, Herpes simplex virus, etc. Akukho cross-reactivity kunye nezinye pathogens ebangela isifo irhashalala.Akukho cross-reactivity kunye genomic DNA yomntu.
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo zePCR ze-ABI 7500 I-QuantStudio® 5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler