Iintlobo ezili-15 ze-Human Papillomavirus E6/E7 Gene mRNA enobungozi obuphezulu
Igama lemveliso
Iintlobo ze-HWTS-CC005A-15 ze-Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko yenye yezona ntlobo zixhaphakileyo zomhlaza wabasetyhini kwihlabathi liphela, kwaye ukuvela kwawo kunxulumene kakhulu neentsholongwane ze-human papilloma (HPV), kodwa lincinci kuphela inani losulelo lwe-HPV elinokuthi libe ngumhlaza. I-HPV enobungozi kakhulu yosulela iiseli ze-epithelial zomlomo wesibeleko kwaye ivelise ii-oncoproteins ezimbini, i-E6 kunye ne-E7. Le protein inokuchaphazela iintlobo ngeentlobo zeeproteni zeseli (ezifana neeproteni ze-tumor suppressor pRB kunye ne-p53), yolule umjikelo weseli, ichaphazele ukwenziwa kwe-DNA kunye nokuzinza kwe-genome, kwaye iphazamise iimpendulo zomzimba zokulwa ne-antiviral kunye ne-antitumor.
Itshaneli
| Itshaneli | Icandelo | Uvavanyo lwe-genotype |
| I-FAM | I-HPV Reaction Buffer 1 | I-HPV16, 31, 33, 35, 51, 52, 58 |
| I-VIC/HEX | Ijini ye-β-actin yomntu | |
| I-FAM | I-HPV Reaction Buffer 2 | I-HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| I-VIC/HEX | I-INS gene yomntu |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-swab yomlomo wesibeleko |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-TimeIinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusebenzisa. Umthamo wokucoca ocetyiswayo yi-50μL. Ukuba isampuli ayigaywanga ngokupheleleyo, yibuyisele kwinyathelo lesi-4 ukuze ihlaziywe kwakhona. Emva koko vavanya ngokwemiyalelo yokusebenzisa.
I-reagent yokukhupha ecetyiswayo: I-RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa ngokungqongqo (Kwinyathelo lesi-5, phinda kabini uxinzelelo lwesisombululo esisebenzayo se-DNaseI, oko kukuthi, thatha i-20μL yesisombululo sesitokhwe se-RNase-Free DNaseI (1500U) kwityhubhu entsha ye-RNase-Free centrifuge, yongeza i-60μL ye-RDD buffer, kwaye udibanise kancinci). Umthamo wokucoca ocetyiswayo yi-60μL. Ukuba isampuli ayigaywanga ngokupheleleyo, yibuyisele kwinyathelo lesi-5 ukuze ihlaziywe kwakhona. Emva koko uvavanye ngokwemiyalelo yokusetyenziswa.
