Iindidi ze-15 ze-Papillomavirus yoMntu oMngcipheko ophezulu we-E6/E7 Gene mRNA
Igama lemveliso
HWTS-CC005A-15 Iintlobo zePapillomavirus yoMntu esemngciphekweni ophezulu E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko lolona hlobo luxhaphakileyo lomhlaza wabasetyhini kwihlabathi jikelele, kwaye ukwenzeka kwawo kunxulumene ngokusondeleyo ne-human papillomaviruses (HPV), kodwa liqaqobana elincinane losulelo lwe-HPV elinokuthi libe ngumhlaza. Umngcipheko ophezulu we-HPV wosulela iiseli ze-epithelial zomlomo wesibeleko kwaye uvelise ii-oncoprotein ezimbini, i-E6 kunye ne-E7. Le proteni inokuchaphazela iindidi zeeprotheni zeselula (ezifana ne-tumor suppressor proteins pRB kunye ne-p53), ukwandisa umjikelo weseli, ichaphazela i-DNA synthesis kunye nokuzinza kwe-genome, kwaye iphazamise iimpendulo ze-antiviral kunye ne-antitumor immune.
Umjelo
| Umjelo | Icandelo | I-Genotype ivavanyiwe |
| FAM | I-HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Ijini ye-β-actin yomntu | |
| FAM | I-HPV Reaction Buffer 2 | HPV 18,39,45,53,56,59,66,68 |
| VIC/HEX | Ufuzo lwe-INS lomntu |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Iswabhu yomlomo wesibeleko |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR SystemIinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo we-elution ocetyiswayo yi-50μL. Ukuba isampulu ayigatyiswanga ngokupheleleyo, yibuyisele kwinyathelo lesi-4 lokwetyisa kwakhona. Uze uvavanye ngokwemiyalelo yokusetyenziswa.
I-reagent yokutsalwa ecetyiswayo: I-RNAprep iPure Animal Tissue Total RNA Extraction Kit (DP431). Ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa ngokungqongqo (Kwinqanaba lesi-5, phinda kabini ukuxinana kwesisombululo sokusebenza se-DNaseI, oko kukuthi, thatha i-20μL ye-RNase-Free DNaseI (1500U) isisombululo sesitokhwe kwityhubhu entsha ye-RNase-Free ye-centrifuge, yongeza i-60μL ye-RDD buffer, kwaye udibanise ngobunono). Umthamo we-elution ocetyiswayo yi-60μL. Ukuba isampulu ayigatyiswanga ngokupheleleyo, yibuyisele kwinyathelo lesi-5 lokwetyisa kwakhona. Uze uvavanye ngokwemiyalelo yokusetyenziswa.
