Iindidi ezili-15 ze-papillomavirus emngciphekweni omkhulu kwi-E6 / E7 NEA Gene

Iindidi ezili-15 ze-papillomavirus emngciphekweni omkhulu we-E6 / e7 gene gene off

Inkcazo emfutshane:

Le kit ijolise ekufumanekeni okuphezulu kwe-15 kwi-papillomavirus ephezulu ye-15 (i-HPV) i-E6 / E7 gene Gene Peneral kwiPlevix yeCervix yomfazi.

Thumela i-imeyile kuthi

Iinkcukacha zemveliso

Iimpawu zeMveliso

Igama lemveliso

I-HWTS-CC005A-15 yeentlobo ze-papillomavirus emngciphekweni omkhulu kwi-E6 / E7 Gene Gene Shonection Tiki (Fluorescence PCR)

Isipikumu

Umhlaza womlomo wesibeleko yenye yezona ntlobo ziqhelekileyo zomhlaza oqhelekileyo womhlaza we-World, kwaye imeko yayo ye-HPV), kodwa incinci kuphela yosulelo lwe-HPV lunokuphuhlisa ube ngumhlaza. Umngcipheko omkhulu we-HPV usulele iiseli zesibeleko esisibeleko kwaye uvelisa ii-oxilpropropropetins ezimbini, e6 kunye ne-E7. Le proteni inokuchaphazela iintlobo ngeentlobo zeeproteni zeselula (ezinjengee-pro proteressor iproteyisi kunye ne-P53), yandisa umjikelo weseli, ichaphazela uzinzo lwe-genom kunye ne-genome, kwaye iphazamisane namajoni omzimba kunye ne-antitmimor.

Ijelo

Ijelo	Icandelo	I-genotype ivavanywa
Indlala	I-HPV yokuphendula i-Buffer 1	I-HPV16,31,33,35,51,51,52,58
I-vic / hex	I-HPV yokuphendula i-Buffer 1	I--β-βin gene
Indlala	I-HPV yokuphendula i-Buffer 2	I-HPV 18,39,45,53,56,59,66,68
I-vic / hex	I-HPV yokuphendula i-Buffer 2	I-gen gen

Iiparamitha zobugcisa

Ukugcinwa	Ulwelo: ≤-18 ℃
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo locaphu	I-wirvical swab
Ct	≤38
CV	<5.0%
Indawo yokuhlala	Iikopi ezingama-500 / ml
Izixhobo ezifanelekileyo	Faka isicelo se-biosystslates 700 ye-PCR ye-PCR ye-PCRFaka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR I-CARTUDIONIO®5 I-PAY-Time-Times Spish Spish I-Slan-96p yangempela ye-PCR ye-PCR I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR

Ukuhamba komsebenzi

Ukucetyiswa Ukuqulunqwa kwe-Reagent: I-Macro kunye ne-Macro kunye ne-Murical Vi / i-HWSE KIT (HWTS-3020-50) NguJiangssu Macro & HECME COED-TOPE. . Ivolumu yesiqhelo yesiqhelo yi-8μl. Ukuba isampulu ayiphelelwanga ngokupheleleyo, yibuyisele kwiNyathelo 4. Kwaye emva koko uvavanyo ngokwemiyalelo yokusetyenziswa.

Ukucetyiswa ukwenziwa kwakhona Ukukhutshwa kufuneka kuqhutywa ngokwemiyalelo yokusetyenziswa ngokungqongqo (kwiNyathelo lesi-5, kabini isisombululo somsebenzi seDnasei, oko kukuthi, i-20μi) ye-rante-free ye-ceberifuge ityhubhu entsha ye-RNAIR kwi-ceberifuge i-ceberifuge i-ceberifuge ityhubhu entsha ye-RNAIRE-FINTAFIFIFA) Yongeza i-60μl ye-rdd buffer, kwaye udibanise kancinci). Ivolumu yesiqhelo yesiqhelo yi-60μl. Ukuba isampulu ayiphelelwanga ngokupheleleyo, yibuyisele kwiNyathelo le-5. Kwaye emva koko uvavanyo ngokwemiyalelo yokusetyenziswa.

Ngaphambili: I-papillomavirus yomntu (iindidi ezingama-28) ze-genotyping

Okulandelayo: Intsholongwane kaGawulayo 1/2 antidy

Bhala umyalezo apha kwaye uthumele kuthi