Iintlobo ezili-18 zeNucleic Acid yeHuman Papilloma Virus enobungozi obuphezulu
Igama lemveliso
Iintlobo ze-HWTS-CC018B-18 ze-Human Papilloma Virus Nucleic Acid Detection Kit (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zibonise ukuba usulelo oluqhubekayo kunye nosulelo oluninzi lwe-human papillomavirus zezinye zezona zizathu zibalulekileyo zomhlaza womlomo wesibeleko.
Usulelo lwe-HPV kwindlela yokuzala luqhelekile phakathi kwabasetyhini abaphila ubomi bokwabelana ngesondo. Ngokwezibalo, ama-70% ukuya kuma-80% abasetyhini banokuba nosulelo lwe-HPV kube kanye ubuncinane ebomini babo, kodwa uninzi losulelo luzithintela ngokwalo, kwaye ngaphezulu kwama-90% abasetyhini abosulelekileyo baya kuba nempendulo esebenzayo yomzimba yokuzikhusela enokuphelisa usulelo phakathi kweenyanga ezi-6 ukuya kwezingama-24 ngaphandle kokungenelela kwezempilo ixesha elide. Usulelo lwe-HPV oluhlala lukhona olunobungozi obukhulu luyimbangela ephambili ye-neoplasia ye-intraepithelial yomlomo wesibeleko kunye nomhlaza womlomo wesibeleko.
Iziphumo zophando lwehlabathi lonke zibonise ukuba ubukho be-HPV DNA enobungozi obukhulu bufunyenwe kwi-99.7% yezigulane ezinomhlaza womlomo wesibeleko. Ke ngoko, ukufunyanwa kwangethuba kunye nokuthintela i-HPV yomlomo wesibeleko yeyona nto ibalulekileyo ekuthinteleni umhlaza. Ukusekwa kwendlela elula, ecacileyo nekhawulezayo yokuxilonga izifo kubaluleke kakhulu ekuxilongweni komhlaza womlomo wesibeleko.
Itshaneli
| I-FAM | I-HPV 18 |
| I-VIC (HEX) | I-HPV 16 |
| I-ROX | I-HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82 |
| CY5 | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Swab yoMlomo, I-Swab yoBufazi, Umchamo |
| Ct | ≤28 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-300/mL |
| Ukuchaneka | （1）Izinto eziphazamisayo Sebenzisa izixhobo zokuvavanya ezi zinto zilandelayo eziphazamisayo, iziphumo zonke azilunganga: i-hemoglobin, iiseli ezimhlophe zegazi, i-cervical mucus, i-metronidazole, i-Jieryin lotion, i-Fuyanjie lotion, i-human lubricant.(2) Ukusebenza ngokunqamlezayo Sebenzisa ezi zixhobo ukuvavanya ezinye iintsholongwane ezinxulumene neendlela zokuzala kunye ne-DNA ye-genomic yabantu enokuba ne-cross-reactivity kunye neekiti, iziphumo zonke azilunganga: Iisampulu ze-HPV6 ezilungileyo, iisampulu ze-HPV11 ezilungileyo, iisampulu ze-HPV40 ezilungileyo, iisampulu ze-HPV42 ezilungileyo, iisampulu ze-HPV43 ezilungileyo, iisampulu ze-HPV44 ezilungileyo, iisampulu ze-HPV54 ezilungileyo, iisampulu ze-HPV67 ezilungileyo, iisampulu ze-HPV69 ezilungileyo, iisampulu ze-HPV70 ezilungileyo, iisampulu ze-HPV71 ezilungileyo, iisampulu ze-HPV72 ezilungileyo, iisampulu ze-HPV81 ezilungileyo, iisampulu ze-HPV83 ezilungileyo, uhlobo lwentsholongwane ye-herpes simplex Ⅱ, i-treponema pallidum, i-ureaplasma urealyticum, i-mycoplasma hominis, i-candida albicans, i-neisseria gonorrhoeae, i-trichomonas vaginalis, i-chlamydia trachomatis kunye ne-human genomic DNA. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler®Iinkqubo ze-PCR zeXesha langempela ezingama-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Isisombululo se-PCR iyonke
Ukhetho 1.
1. Ukuthatha iisampulu
2. Ukukhupha i-Nucleic acid
3. Yongeza iisampulu kumatshini
Ukhetho 2.
1. Ukuthatha iisampulu
2. Ayinakukhutshwa
3. Yongeza iisampulu kumatshini
