Iintlobo ezingama-28 ze-HPV Nucleic Acid Detection Kit (Fluorescence PCR)
Igama lemveliso
Iintlobo ezingama-28 ze-HPV Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Human Papillomavirus (HPV) ikwintsapho yePapillomaviridae yentsholongwane ye-DNA encinci, engagqunywanga, ejikelezayo enemicu emibini, enobude be-genome obumalunga ne-8000 base pairs (bp). I-HPV yosulela abantu ngokudibana ngqo okanye ngokungathanga ngqo nezinto ezingcolisiweyo okanye ukudluliselwa ngokwesondo. Le ntsholongwane ayijoliswanga nje kuphela kumninimzi, kodwa ikwajoliswa nakwizicubu ezithile, kwaye inokosulela kuphela ulusu lomntu kunye neeseli ze-epithelial ze-mucosal, nto leyo ebangela iintlobo ngeentlobo zee-papillomas okanye ii-warts eluswini lomntu kunye nomonakalo okhulayo kwi-epithelium yokuzala.
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zibonise ukuba usulelo oluqhubekayo lwe-HPV kunye nosulelo oluninzi zezinye zezona zizathu ziphambili zomhlaza womlomo wesibeleko. Okwangoku, unyango olusebenzayo oluqatshelweyo alusekho kumhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV yeyona nto iphambili ekuthinteleni umhlaza womlomo wesibeleko. Kubaluleke kakhulu ukumisela uvavanyo olulula, olucacileyo nolukhawulezayo lokuxilonga i-etiology yokuxilongwa kweklinikhi kunye nonyango lomhlaza womlomo wesibeleko.
Itshaneli
| S/N | Itshaneli | Uhlobo |
| I-PCR-Mix1 | I-FAM | 16, 18, 31, 56 |
| I-VIC(HEX) | Ulawulo lwangaphakathi | |
| CY5 | 45, 51, 52, 53 | |
| I-ROX | 33, 35, 58, 66 | |
| I-PCR-Mix2 | I-FAM | 6, 11, 54, 83 |
| I-VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| I-ROX | 39, 59, 68, 73 |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤ -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli yomchamo, Isampuli yeswabhu yomlomo wesibeleko yabasetyhini, Isampuli yeswabhu yomlomo wesibeleko yabasetyhini |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-300/mL |
| Ukuchaneka | Akukho mpendulo ihambelanayo nezinye izifo ezinxulumene nendlela yokuzala kunye ne-DNA ye-genomic yomntu. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, Iinkqubo ze-PCR ze-QuantStudio®5 Real-Time, Iinkqubo ze-PCR ze-SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.), Iinkqubo ze-PCR ze-LightCycler®480 Real-Time, Iinkqubo ze-PCR ze-LineGene 9600 Plus Real-Time Detection (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Iinkqubo ze-PCR ze-BioRad CFX96 Real-Time, Iinkqubo ze-PCR ze-BioRad CFX Opus 96 Real-Time. |
Ukuhamba komsebenzi: Jonga iKit IFU ukuze ufumane iinkcukacha
Isisombululo 1 (Ukukhupha i-nucleic acid ngokuzenzekelayo)
Isisombululo sesi-2: (I-Macro kunye ne-Micro-Test Sample Release Reagent)
Ii-reagents ziyafuneka kodwa azibonelelwa
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), i-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwa
Iityhubhu ezingenayo i-DNase/RNase eziyi-1.5mL, ii-tips ezingenayo i-DNase/RNase, ii-strips ezineetyhubhu ezi-8 ze-PCR, i-tabletop centrifuge, i-tabletop shaker mixer.
