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    • Iindidi ezi-4 zeeNtsholongwane zokuPhumla i-Nucleic Acid

    Iindidi ezi-4 zeeNtsholongwane zokuPhumla kwi-Nucleic Acid Umfanekiso obonisiwe
    • Iindidi ezi-4 zeeNtsholongwane zokuPhumla i-Nucleic Acid

    Inkcazelo emfutshane:

    Le khithi isetyenziselwa ukufunyaniswa komgangatho we-SARS-CoV-2, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B kunye nokuphefumla kwe-sycytial virus nucleic acids kwiisampulu ze-oropharyngeal swab.


    Iinkcukacha zeMveliso

    Iithegi zeMveliso

    Igama lemveliso

    I-HWTS-RT075-Umkhenkce-zomisiweyo ezi-4 Iindidi zeeVirusi eziphefumlayo zeNucleic Acid Detection Kit (Fluorescence PCR)

    I-Epidemiology

    Isifo se-Corona Virus 2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwinyumoniya ebangelwa lusulelo lwe-SARS-CoV-2.I-SARS-CoV-2 yi-coronavirus yodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi.Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-SARS-CoV-2, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7.Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa.Izigulana ezimbalwa zineempawu ezinjengokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye nohudo, njl.

    Umkhuhlane, owaziwa ngokuba "ngumkhuhlane", sisifo esosulelayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane.Iyosulela kakhulu.Isasazwa ikakhulu ngokukhohlela kunye nokuthimla.Idla ngokuqhambuka entwasahlobo nasebusika.Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A (IFV A), umkhuhlane B (IFV B), kunye noMkhuhlane C (IFV C) iindidi ezintathu, zonke zezentsholongwane encangathi, zibangela isifo ebantwini ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yinto enye. -i-stranded, i-virus ye-RNA ecandiweyo.Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele."Shift" ibhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kwentsholongwane entsha "subtype".Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini, iYamagata kunye neVictoria.Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye iyayiphepha iliso lenkqubo yomzimba yokuzikhusela kunye nokuphelisa ngokuguqulwa kwayo.Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu.Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.

    Intsholongwane yokuphefumla ye-syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae.Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana.Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali kunye nenyumoniya, ehambelana ne-asthma ebantwaneni.Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia.Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl.

    Umjelo

    FAM I-SARS-CoV-2
    VIC(HEX) RSV
    CY5 IFV A
    ROX IFV B
    NED

    Ulawulo lwangaphakathi

    IiParameters zobuGcisa

    Ugcino 2-8°C
    Beka ubomi kwishelufa Iinyanga ezili-12
    Uhlobo loMfanekiso I-Oropharyngeal swab
    Ct ≤38
    LoD I-SARS-CoV-2: 150Iikopi/mL

    Intsholongwane ye-Influenza A/influenza B virus/Respiratory syncytial virus: 300Copies/mL
    Ukuchaza ngokuthe ngqo Akukho-cross-reactivity with human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia inyumoniya, metapneumovirus yabantu, enterovirus A, B, C, D, intsholongwane yemiphunga yomntu, intsholongwane ye-epstein-barr, intsholongwane yemasisi, intsholongwane ye-cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kunye ne-cryptococcus esanda kuzalwa kunye ne-nucleic acid yabantu.
    Izixhobo Ezisebenzayo I-Biosystems eSetyenzisiweyo 7500 Inkqubo ye-PCR yexesha langempela, i-Biosystems eSetyenzisiweyo 7500 Iinkqubo ze-PCR ezikhawulezayo, i-QuantStudio®5 ye-Real-Time PCR Systems

    Ukuhamba komsebenzi

    Ukhetho loku-1.

    I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

    Ukhetho lwesi-2.

    I-Reagent yokutsalwa ecetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.

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