Iindidi ezi-4 zeeNtsholongwane zokuPhumla i-Nucleic Acid

Iindidi ezi-4 zeeNtsholongwane zokuPhumla kwi-Nucleic Acid eFakelweyo Umfanekiso

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufunyaniswa komgangatho we-SARS-CoV-2, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B kunye nokuphefumla kwe-sycytial virus nucleic acids kwiisampulu ze-oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT186- Iindidi ezi-4 zeeNtsholongwane zokuPhumla iiNucleic Acid Detection Kit (Fluorescence PCR)

I-Epidemiology

Isifo se-Corona Virus 2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwinyumoniya ebangelwa lusulelo lwe-SARS-CoV-2. I-SARS-CoV-2 yi-coronavirus yodidi lwe-β. I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-SARS-CoV-2, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa. Izigulana ezimbalwa zineempawu ezinjengokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye nohudo, njl.

Umkhuhlane, owaziwa ngokuba "ngumkhuhlane", sisifo esosulelayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane. Iyosulela kakhulu. Isasazwa ikakhulu ngokukhohlela kunye nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A (IFV A), umkhuhlane B (IFV B), kunye noMkhuhlane C (IFV C) iintlobo ezintathu, zonke zezentsholongwane encangathi, zibangela isifo somntu ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yintsholongwane enye, i-RNA ecandekileyo. Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele. "Shift" ibhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kwentsholongwane entsha "subtype". Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini, iYamagata kunye neVictoria. Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye iyayiphepha iliso lenkqubo yomzimba yokuzikhusela kunye nokuphelisa ngokuguqulwa kwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu. Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.

Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae. Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali kunye nenyumoniya, ehambelana ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl.

Umjelo

FAM	I-SARS-CoV-2
VIC(HEX)	RSV
CY5	IFV A
ROX	IFV B
NED	Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino	2-8°C
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	I-Oropharyngeal swab
Ct	≤38
LoD	I-SARS-CoV-2: 150Iikopi/mL Intsholongwane ye-Influenza A/influenza B virus/Respiratory syncytial virus: 300Copies/mL
Ukuchaza ngokuthe ngqo	Akukho cross-reactivity kunye ne-coronavirus yabantu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus uhlobo 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniaeumoviruse, intsholongwane kaGawulayo, i-human virus, i-metapneumoviruse, i-human intsholongwane yemiphunga, intsholongwane ye-epstein-barr, intsholongwane yemasisi, intsholongwane ye-cytomegalo yabantu, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniae, i-s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kunye ne-cryptococcus esanda kuzalwa kunye ne-nucleic acid yabantu.
Izixhobo Ezisebenzayo	I-Biosystems eSetyenzisiweyo 7500 Inkqubo ye-PCR yexesha langempela, i-Biosystems eSetyenzisiweyo 7500 Iinkqubo ze-PCR ezikhawulezayo, i-QuantStudio®5 ye-Real-Time PCR Systems

Ukuhamba komsebenzi

Ukhetho loku-1.

I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ukhetho lwesi-2.

I-Reagent yokutsalwa ecetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.

Ngaphambili: I-Mycobacterium Tuberculosis Rifampicin Resistance

Okulandelayo: Iindidi ezili-19 zePathogen Nucleic Acid yokuphefumla

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