Iintlobo ezi-4 zeentsholongwane zokuphefumla i-Nucleic Acid
Igama lemveliso
I-HWTS-RT186- Iintlobo ezi-4 zeentsholongwane zokuphefumla Isixhobo sokubona i-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Isifo seCorona Virus sika-2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwi-pneumonia ebangelwa lusulelo lwe-SARS-CoV-2. I-SARS-CoV-2 sisifo se-coronavirus esikwi-β genus. I-COVID-19 sisifo esosulelayo esihlaselayo, kwaye uluntu ngokubanzi lusengozini. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-SARS-CoV-2, kwaye abantu abane-symptomatic nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokukhulelwa liphakathi kweentsuku ezili-1-14, uninzi lwazo iintsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokudinwa zezona mpawu ziphambili. Izigulana ezimbalwa zazineempawu ezifana nokuxinana kweempumlo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo, njl.
I-Influenza, eyaziwa ngokuba yi-"flu", sisifo esosulelayo esibangelwa yintsholongwane ye-influenza. Isasazeka kakhulu. Isasazeka kakhulu ngokukhohlela nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane ze-Influenza zahlulwe zibe yi-influenza A (IFV A), i-influenza B (IFV B), kunye ne-Influenza C (IFV C) iintlobo ezintathu, zonke zezentsholongwane ezinamathelayo, zibangela isifo ebantwini ikakhulu kwiintsholongwane ze-influenza A kunye ne-B, yintsholongwane ye-RNA enemigca enye, ehlukaniswe ngezigaba. Intsholongwane ye-Influenza A sisifo sokuphefumla esikhawulezileyo, kuquka i-H1N1, i-H3N2 kunye nezinye iintlobo, ezinokuthi ziguquke kwaye ziqhambuke kwihlabathi liphela. Igama elithi "Shift" libhekisa kuguquko lwentsholongwane ye-influenza A, nto leyo ebangela ukuvela kwentsholongwane entsha "engaphantsi". Iintsholongwane ze-Influenza B zahlulwe ngemigca emibini, i-Yamagata kunye ne-Victoria. Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye ithintela ukujongwa nokupheliswa kwenkqubo yomzimba yokuzikhusela ngoguquko lwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane ye-influenza B sihamba kancinci kunesentsholongwane ye-influenza A yabantu. Intsholongwane ye-Influenza B inokubangela izifo zokuphefumla ebantwini kwaye ikhokelele kwiindyikityha zobhubhane.
Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, ephuma kusapho lwe-paramyxoviridae. Idluliselwa ngamathontsi omoya kunye nokudibana ngokusondeleyo kwaye yeyona ntsholongwane iphambili yosulelo lwendlela yokuphefumla esezantsi kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis enzima kunye ne-pneumonia, ezinxulumene ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, kubandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima nge-otitis media, i-pleurisy kunye ne-myocarditis, njl. Usulelo lwendlela yokuphefumla ephezulu luphawu oluphambili losulelo kubantu abadala nakubantwana abadala.
Itshaneli
| I-FAM | I-SARS-CoV-2 |
| I-VIC(HEX) | I-RSV |
| CY5 | IFV A |
| I-ROX | IFV B |
| I-NED | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | 2-8°C |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab |
| Ct | ≤38 |
| I-LoD | I-SARS-CoV-2: Iikopi ezili-150/mL Intsholongwane ye-Influenza A/Intsholongwane ye-Influenza B/Intsholongwane ye-Respiratory syncytial: 300Iikopi/mL |
| Ukuchaneka | Akukho reaction-reaction eyenzekayo ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kunye ne-cryptococcus esandul' ukuzalwa kunye ne-human genomic nucleic acid. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time, i-PCR Systems ye-PCR eFast Real-Time, i-QuantStudio®5 Real-Time PCR Systems |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co.,Ltd.








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