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    • Middle East Respiratory Syndrome ICoronavirus Nucleic Acid

    Middle East Respiratory Syndrome Coronavirus Nucleic Acid Featured Image
    • Middle East Respiratory Syndrome ICoronavirus Nucleic Acid

    Inkcazelo emfutshane:

    Ikhithi isetyenziselwa ukufumanisa umgangatho we-MERS coronavirus nucleic acid kwi-nasopharyngeal swabs kunye ne-Middle East Respiratory Syndrome (MERS) coronavirus.


    Iinkcukacha zeMveliso

    Iithegi zeMveliso

    Igama lemveliso

    HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

    I-Epidemiology

    I-Middle East yokuphefumla isifo se-coronavirus (MERS-CoV), i-β-coronavirus ebangelaIsifo sokuphefumla ebantwini, sachongwa okokuqala kwisigulane esineminyaka engama-60 ubudala saseSaudi Arabia esifayo ngomhla we-24 kaJulayi, 2012. Inkcazo yeklinikhi yosulelo lwe-MERS-CoV isuka kwi-asymptomatic state okanye iimpawu zokuphefumula ezithambileyo ukuya kwisifo esibi kakhulu sokuphefumla nokufa.

    Umjelo

    FAM Intsholongwane ye-MERS RNA
    VIC(HEX)

    Ulawulo lwangaphakathi

    IiParameters zobuGcisa

    Ugcino

    ≤-18℃ ebumnyameni

    Beka ubomi kwishelufa

    Iinyanga ezili-9

    Uhlobo loMfanekiso

    Iiswabs ze-nasopharyngeal ezisanda kuqokelela

    CV

    ≤5.0%

    Ct

    ≤38

    LoD

    		 Iikopi ezili-1000/mL

    Ukuchaza ngokuthe ngqo

    Akukho kuphinda kusebenze kunye nee-coronavirus zabantu i-SARSr-CoV kunye nezinye iipathogens eziqhelekileyo.

    Izixhobo Ezisebenzayo:

    		 Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

    Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

    I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

    SLAN-96P Iinkqubo zePCR zeXesha langempela

    Inkqubo yePCR yeLightCycler®480 yeXesha langempela

    I-LineGene 9600 Plus Real-Time PCR Detection System

    MA-6000 Real-Time Quantitative Thermal Cycler

    I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

    BioRad CFX Opus 96 Real-Time PCR System

    Ukuhamba komsebenzi

    Ukhetho loku-1.

    I-Reagent yokutsalwa ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.

    Ukhetho lwesi-2.

    I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

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