I-ALDH Genetic Polymorphism
Igama lemveliso
I-HWTS-GE015Ikhithi yokuFumanisa i-ALDH Genetic Polymorphism (ARMS -PCR)
I-Epidemiology
I-ALDH2 gene (i-acetaldehyde dehydrogenase 2), ifumaneka kwi-chromosome yomntu 12. I-ALDH2 ine-esterase, i-dehydrogenase kunye nomsebenzi we-reductase ngaxeshanye. Izifundo zibonise ukuba i-ALDH2 yi-enzyme ye-metabolic ye-nitroglycerin, eguqula i-nitroglycerin ibe yi-nitric oxide, ngaloo ndlela iphumza imithambo yegazi kwaye iphucule ukuphazamiseka kokuhamba kwegazi. Nangona kunjalo, kukho ii-polymorphisms kwi-ALDH2 gene, ezigxile kakhulu eMpuma Asia. I-ALDH2*1/1 GG yasendle inamandla amakhulu e-metabolic, ngelixa uhlobo lwe-heterozygous lune-6% kuphela yomsebenzi we-enzyme yasendle, kwaye uhlobo lwe-homozygous mutant alunamsebenzi we-enzyme phantse, kunye ne-metabolism ebuthathaka kakhulu kwaye ayinakufezekisa isiphumo esifunekayo, ngaloo ndlela ibangela ingozi emzimbeni womntu.
Itshaneli
| I-FAM | I-ALDH2 |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Igazi elichasene nokugaywa kwegazi yi-EDTA |
| CV | <5.0% |
| I-LoD | 103Iikopi/mL |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents zokukhupha ezicetyiswayo: sebenzisa i-Blood Genome DNA Extraction Kit (DP318) yiTiangen Biotech (Beijing) Co.,Ltd. okanye i-Blood Genome Extraction Kit (A1120) yiPromega ukuze ukhuphe i-EDTA ye-DNA ye-Genomic blood anticoagulated.
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo engqongqo. Umthamo wokucoca ocetyiswayo ngu100μL.
