ALDH Genetic Polymorphism
Igama lemveliso
HWTS-GE015I-ALDH Genetic Polymorphism Kit (ARMS -PCR)
I-Epidemiology
I-ALDH2 gene (i-acetaldehyde dehydrogenase 2), ibeka kwi-chromosome yomntu 12. I-ALDH2 ine-esterase, i-dehydrogenase kunye nomsebenzi we-reductase ngexesha elifanayo. Uphononongo lubonise ukuba i-ALDH2 yi-enzyme ye-metabolic ye-nitroglycerin, eguqula i-nitroglycerin ibe yi-nitric oxide, ngaloo ndlela ikhulula imithwalo yegazi kunye nokuphucula ukuphazamiseka kwegazi. Nangona kunjalo, kukho iipolymorphisms kwi-ALDH2 gene, egxile kakhulu kwi-East Asia. Uhlobo lwasendle lwe-ALDH2 * 1/* 1 GG lunamandla e-metabolism, ngelixa uhlobo lwe-heterozygous lune-6% kuphela ye-enzyme yohlobo lwasendle, kwaye uhlobo lwe-homozygous mutant luphantse lube nomsebenzi we-enzyme, kunye ne-metabolism ebuthathaka kakhulu kwaye ayikwazi ukufikelela kwisiphumo esifunwayo, ngaloo ndlela ibangela umonakalo kumzimba womntu.
Umjelo
| FAM | I-ALDH2 |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Igazi le-EDTA anticoagulated |
| CV | <5.0 |
| LoD | 103Iikopi/mL |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ii-reagents zokutsalwa ezicetyiswayo: sebenzisa i-Blood Genome DNA Extraction Kit (DP318) yi-Tiangen Biotech (Beijing) Co.,Ltd. okanye i-Blood Genome Extraction Kit (A1120) yi-Promega ukukhupha i-EDTA ye-anticoagulated blood Genomic DNA.
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe ngeMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo we-lution ocetyiswayo ngu100μL.
