Intsholongwane yeHepatitis E
Igama lemveliso
HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Intsholongwane yeHepatitis E (HEV) yintsholongwane yeRNA ebangela iingxaki zempilo kwihlabathi.Inoluhlu olubanzi lwamamkeli kwaye inepropati yokuwela imiqobo ye-interspecies.Yenye yezona zinto zibalulekileyo ze-zoonotic pathogens kwaye ibangela ingozi enkulu ebantwini nakwizilwanyana.I-HEV isasazwa ikakhulu ngosulelo lwe-fecal-oral, kwaye inokudluliselwa ngokuthe nkqo ngeembumba okanye ngegazi.Phakathi kwabo, kwindlela yokuhanjiswa kwe-fecal-oral, amanzi angcolileyo e-HEV kunye nokutya kwasasazeka ngokubanzi, kwaye umngcipheko wosulelo lwe-HEV ebantwini nakwizilwanyana uphezulu[1-2].
Umjelo
| FAM | HEV nucleic acid |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Ukuswabha komqala |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Ukuchaza ngokuthe ngqo | Intsholongwane yeHepatitis E (HEV) yintsholongwane yeRNA ebangela iingxaki zempilo kwihlabathi.Inoluhlu olubanzi lwamamkeli kwaye inepropati yokuwela imiqobo ye-interspecies.Yenye yezona zinto zibalulekileyo ze-zoonotic pathogens kwaye ibangela ingozi enkulu ebantwini nakwizilwanyana.I-HEV isasazwa ikakhulu ngosulelo lwe-fecal-oral, kwaye inokudluliselwa ngokuthe nkqo ngeembumba okanye ngegazi.Phakathi kwabo, kwindlela yokuhanjiswa kwe-fecal-oral, amanzi angcolileyo e-HEV kunye nokutya kwasasazeka ngokubanzi, kwaye umngcipheko wosulelo lwe-HEV ebantwini nakwizilwanyana uphezulu[1-2]. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System I-Biosystems eSetyenzisiweyo 7500 Inkqubo yePCR ekhawulezayo yexesha langempela I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufuneka ikhutshwe ngokwemiyalelo.Umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho lwesi-2
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwa yi-Tiangen Biotech (Beijing) Co., Ltd. Kufuneka ikhutshwe ngokungqongqo ngokwemiyalelo.Umthamo wesampulu ekhutshiweyo yi-140μL.Umthamo we-elution ocetyiswayo yi-60µL.v
