I-Gene yoKuchasana neCarbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Igama lemveliso
I-HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescence PCR)
I-Epidemiology
Ii-antibiotics zeCarbapenem zi-antibiotics ezingaqhelekanga ze-β-lactam ezine-spectrum ebanzi kakhulu ye-antibacterial kunye nomsebenzi onamandla kakhulu we-antibacterial. Ngenxa yokuzinza kwayo kwi-β-lactamase kunye ne-toxicity ephantsi, iye yaba yenye yezona ziyobisi zibalulekileyo ze-antibacterial kunyango lwezifo ezinzulu zebhaktiriya. IiCarbapenems zizinzile kakhulu kwi-plasmid-mediated extended-spectrum β-lactamases (ESBLs), ii-chromosomes, kunye ne-plasmid-mediated cephalosporinases (ii-AmpC enzymes).
Itshaneli
| Umxube we-PCR 1 | Umxube we-PCR 2 | |
| I-FAM | I-IMP | I-VIM |
| I-VIC/HEX | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
| CY5 | I-NDM | I-KPC |
| I-ROX | I-OXA48
| I-OXA23 |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-sputum, iikholoni ezicocekileyo, i-rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | 103I-CFU/mL |
| Ukuchaneka | a) Le khithi ibona iireferensi ezingalunganga zenkampani ezisemgangathweni, kwaye iziphumo ziyahlangabezana neemfuno zeereferensi ezifanelekileyo. b) Iziphumo zovavanyo lwe-cross-reactivity zibonisa ukuba le khithi ayinayo i-cross reaction nezinye izifo ezibangela ukuphefumla, ezifana neKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, okanye iisampulu eziqulethe ezinye ii-genes ezinganyangekiyo ngamayeza i-CTX, mecA, SME, SHV, TEM, njl. c) Izinto ezichasene nokuphazamiseka: I-Mucin, i-Minocycline, i-Gentamicin, i-Clindamycin, i-Imipenem, i-Cefoperazone, i-Meropenem, i-Ciprofloxacin Hydrochloride, i-Levofloxacin, i-Clavulanic acid, i-Roxithromycin zikhethiwe ukuze kuvavanywe ukuphazamiseka, kwaye iziphumo zibonisa ukuba ezi zinto ziphazamisayo zikhankanyiweyo apha ngasentla azinazo iziphumo eziphazamisayo ekufumaneni ii-genes zokumelana ne-carbapenem KPC, NDM, OXA48, OXA23, VIM, kunye ne-IMP. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela (I-FQD-96A，IHangzhouItekhnoloji yeBioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Macro kunye ne-Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetyenziswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Yongeza i-200μL yesaline eqhelekileyo kwi-thallus precipitate. Amanyathelo alandelayo kufuneka alandele imiyalelo yokukhupha, kwaye umthamo wokucoca ocetyiswayo ngu100μL.
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co., Ltd. Ukukhupha kufuneka kuqalwe ngokuhambelana nenyathelo lesi-2 lomyalelo wokusetyenziswa (yongeza i-200μL ye-buffer GA kwi-thallus precipitate, uze uyishukumise ide i-thallus imiswe ngokupheleleyo). Sebenzisa amanzi angena-RNase/DNase ukuze ukhuphe, kwaye umthamo wokukhupha ocetyisiweyo yi-100μL.
Ukhetho 3.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Sample Release Reagent. Isampuli ye-sputum kufuneka ihlanjwe ngokongeza i-1mL ye-saline eqhelekileyo kwi-thallus precipitate ekhankanyiweyo apha ngasentla, ifakwe kwi-centrifuge kwi-13000r/min imizuzu emi-5, kwaye i-supernatant ilahlwe (gcina i-10-20µL ye-supernatant). Kwi-colony ecocekileyo kunye ne-rectal swab, yongeza i-50μL ye-sample release reagent ngqo kwi-thallus precipitate ekhankanyiweyo apha ngasentla, kwaye amanyathelo alandelayo kufuneka akhutshwe ngokwemiyalelo yokusetyenziswa.
