I-Carbaecloction Dege (i-KPC / ye-KPC / NDM 48 / i-AXA 23 / VM / i-FART)
Igama lemveliso
I-HWTS-OT045 I-CACKAEPUDY DEET (KPC / NDM / I-ANDA 48 / I-AX5 / I-AX5 / VIM / I-FRCR)
Isipikumu
I-carbapech ene-antibiotics i-anypical i-anypical β-lactam antibiotics kunye ne-vibiotics ebanzi kunye nomsebenzi onamandla owomeleleyo. Ngenxa yozinzo lwayo kwi-β-lactanase kunye netyhefu esezantsi, iba lelinye lawona machiza abaluleke kakhulu kakhulu kunyango olunzima lwentsholongwane. I-Carbapenems zizinzile kakhulu kwi-plasmid-spectrum-lacamases (i-ecbls), i-chromosomes, kunye ne-plasmid-med-med-candsporinases (Ampc Enzymes).
Ijelo
| I-PCR-xube 1 | I-PCR-xube 2 | |
| Indlala | Chaza | Vim |
| I-vic / hex | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
| I-Cy5 | I-NDM | Kpc |
| Rox | I-OXA48
| I-OXA23 |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | I-Sputum, iikholoni ezicocekileyo, i-wincital swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Indawo yokuhlala | 103I-CFU / ML |
| Ubuchule | a) Ikhithi ifumana i-iting ufumana izikhombisi ezingekho mthethweni, kwaye iziphumo ziyahlangabezana neemfuno zezalathiso ezihambelanayo. b) Iziphumo zovavanyo lokuphinda-phinda lokuhlaselwa kwakhona zibonisa ukuba le kit ayinantsingiselo yokuphendula kunye nezinye izinto zokuphefumla, i-Aisserbas Aerumoniae, i-proptoonas bauumonia, i-staudorococs aurea, owytoca, I-haemophilis ifluelweni, i-acinetobacter junii, i-apinetobacter haemolytica, i-chaomylla puonesia, interpiyas, i-chinanocovia pleomoniae, i-chinanocovia pleomonia, okanye iisampulu eziqulathe ezinye iintlobo zeziyobisi ezinganyangekiyo I-CTX, MECA, iSM, SOT, njl. c) Ukuphazamiseka kwe-antin, i-minecycline, i cefofycin, i-cefofrazucn, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo zichazwe apha ngasentla azinantsingiselo yokuphendula ukufunyanwa kwe I-Carbaeclocy Gees Ukunganyangeki kwe-KPC, i-NDM, e-Onqua48, i-OXA23, VIM, NOP. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR I-CARTUDIONIO®5 I-PAY-Time-Times Spish Spish I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Tlinegene 9600 kunye neNkqubo yokufumana iPCR ye-PCR (Fqd-96a,HangzhouItekhnoloji ye-bioer) I-Ma-6000 yexesha lokwenyani le-thermal ye-thermal (Suzhou Molarray Co., Ltd.) I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
Ukucetyiswa Ukuqhekeza Ukuphinda uqwalasele kwakhona: I-Macro kunye ne-Macro kunye ne-Macro kunye ne-RNA ye-DNA / HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (enokusetyenziswa nge-macro & micro-micro-stackic ye-nucleid ye-nucleid i-acid (HWTS-3006C-3006b)) nge-jiangsis-test Co.p. I-Thalls ichanekile. Amanyathelo alandelayo makalandele imiyalelo yokukhupha, kwaye ivolumu yesiqhelo ye-Exel10I-0μl.
Inketho 2.
Ukucetyiswa ukwenziwa komgangatho: Ukukhutshwa kwe-nucleic acid (YDPUP) nge-tiangen biotech (iBeijing) CO. , kwaye shake de ibe i-thalls inqunyanyisiwe ngokupheleleyo). Sebenzisa amanzi e-rnase / dnase yasimahla ye-elution, kunye nevolumu ye-ECRAMPMED i-100μl.
Inketho 3.
Ukucetyiswa ukuqulunqwa kwe-Reagent: I-Macro kunye neMacro kunye neMicroral-Page yeSampulu. Isampulu ye-Sputum kufuneka ihlanjwe ngo-1ml ye-Suline yesiqhelo kwi-thalls echazwe apha ngasentla ye-thalls, i-Getynaged nge-5000r imizuzu emi-5, kwaye igcinwe i-10 ukuya kwi-10-20μl ye-supernatant). Ukufumana i-collony kunye ne-swabl evuthayo, yongeza i-50μl yesampula yokukhutshwa kwesampulu ngokuthe ngqo kuThaltys olichazwe apha ngasentla, kwaye amanyathelo alandelayo kufuneka akhutshwe ngokwemiyalelo yokusetyenziswa.
