Umkhuhlane A Virus Universal/H1/H3
Igama lemveliso
I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) yeyona microorganism encinci yeprokaryotic enesakhiwo seseli kwaye akukho ludonga lweseli phakathi kwebhaktheriya kunye neentsholongwane.I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla ebantwini, ngakumbi ebantwaneni nakubantu abaselula.I-MP inokubangela i-Mycoplasma hominis pneumonia, izifo zentsholongwane yokuphefumula ebantwaneni kunye ne-atypical pneumonia.Iimpawu zeklinikhi ziyahlukahlukana, ubukhulu becala ukhohlokhohlo oluqatha, umkhuhlane, ukugodola, intloko ebuhlungu, umqala obuhlungu, usulelo lwendlela yokuphefumla ephezulu kunye ne-bronchopneumonia zezona zixhaphakileyo.Ezinye izigulana zinokuba nenyumoniya emandundu ngenxa yosulelo lwendlela yokuphefumla ephezulu, kunye noxinzelelo olukhulu lokuphefumla okanye nokufa.I-MP ingenye yezifo eziqhelekileyo kunye nezibalulekileyo kwi-pneumonia efunyenwe kuluntu (CAP), i-akhawunti ye-10% -30% ye-CAP, kwaye umlinganiselo unokunyusa amaxesha angama-3-5 xa i-MP ixhaphake.Kwiminyaka yakutshanje, inani leMP kwi-CAP pathogens liye lanyuka ngokuthe ngcembe.Iziganeko zokusuleleka kwe-Mycoplasma pneumoniae ziye zanda, kwaye ngenxa yokungabonakali kwayo kweklinikhi, kulula ukubhidaniswa ne-bacterial and viral cold.Ke ngoko, ukufunyaniswa kwangoko kwelebhu kubaluleke kakhulu kuxilongo lwezonyango kunye nonyango.
Umjelo
| FAM | umkhuhlane Uhlobo lwentsholongwane yendalo yonke i-nucleic acid |
| VIC/HEX | umkhuhlane A H1 uhlobo lwentsholongwane nucleic acid |
| ROX | umkhuhlane A H3 uhlobo lwentsholongwane nucleic acid |
| CY5 | ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye nezinye iisampuli zokuphefumla ezifana Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2 I-B1 / B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njl. kunye ne-DNA ye-genomic yabantu. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-Nucleic Acid Extraction okanye iReagent yokuCoca (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-140μL, kwaye umthamo we-lution ocetyiswayo yi-60μL.
Ukhetho lwesi-2.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
