Intsholongwane yeDengue, iZika Virus kunye neChikungunya Virus Multiplex
Igama lemveliso
HWTS-FE040 Virus Dengue, Zika Virus and Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Dengue fever (DF), ebangelwa yintsholongwane yedengue (DENV) usulelo, sesinye sezifo ezisulelayo ze-arbovirus. Uthungelwano lwayo lubandakanya i-Aedes aegypti kunye ne-Aedes albopictus. I-DF ixhaphake kakhulu kwimimandla yetropikhi kunye neyetropikhi. I-DENV yeye-flavivirus phantsi kwe-flaviviridae, kwaye ingahlelwa kwii-serotypes ze-4 ngokwe-antigen yomhlaba. Iimpawu zeklinikhi zosulelo lwe-DENV ikakhulu ziquka intloko ebuhlungu, umkhuhlane, ubuthathaka, ukwanda kwe-lymph node, i-leukopenia kunye nokunye, kunye nokopha, ukothuka, ukwenzakala kwesibindi okanye ukufa kwiimeko ezinzima. Kwiminyaka yakutshanje, utshintsho lwemozulu, ukufudukela kwabantu ezidolophini, uphuhliso olukhawulezileyo lokhenketho kunye nezinye izinto zibonelele ngeemeko ezikhawulezayo neziluncedo zokusasaza kunye nokusasazwa kwe-DF, ekhokelela ekwandeni okuthe gqolo kobhubhane we-DF.
Umjelo
| FAM | DENV nucleic acid |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Iserum entsha |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Iikopi ezingama-500/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lokuphazanyiswa zibonisa ukuba xa ukuxinwa kwe-bilirubin kwi-serum kungekho ngaphezu kwe-168.2μmol / ml, i-hemoglobin concentration eveliswa yi-hemolysis ayikho ngaphezu kwe-130g / L, i-concentration ye-lipid yegazi ayikho ngaphezu kwe-65mmol / ml, i-IgG yoxinzelelo kwi-serum ayikho ngaphezu kwe-5mg / mL yokufumanisa i-virus okanye i-virus ye-chikungue. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus kunye neesampulu zabantu genomic serum zikhethwa uvavanyo cross-reactivity, kwaye iziphumo zibonisa ukuba akukho reaction cross phakathi le kit kunye pathogens ezikhankanywe ngasentla. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), kwaye ukutsalwa kufuneka kuqhutywe ngokuhambelana ngqongqo nomyalelo wokusetyenziswa. Umthamo wesampulu ekhutshiweyo yi-140μL, kwaye umthamo we-elution ocetyiswayo yi-60μL.
Ukhetho lwesi-2.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HCTS0, HWTS-30BTS) yiMacro & Micro-Test Med-Tech Co., Ltd., kunye nokutsalwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
