Intsholongwane yeDengue, intsholongwane ye-ZIKA kunye ne-chikunyoy yentsholongwane
Igama lemveliso
I-HWTS-FE040 Dengue Virus, Intsholongwane yeVikta kunye neChikungo virus ye-Inyx In Bucleid iKid Ikhithi ye-Acid iKid (Fluorescence PCR)
Isipikumu
I-Dengue Fever (DF), ethi ikhutshwe yi-Dengue Virus (i-deniv) yosulelo, yenye yezona zifo ze-Arbovirus zeArbovirus. Iphakathi sayo sokuhambisa ibandakanya i-AEDES AEGYPTI kunye ne-AEDES ALBOPICTUS. I-DF ikakhulu ixhaphake kwiindawo ezitshisayo nezinzulu. I-denv yekaFlavivirus phantsi kweFlavivididae, kwaye inokuhlelwa kwi-4 serotypes ngokwe-Antigen. Ukubonakaliswa kweklinikhi yosulelo lwe-denv ikakhulu kubandakanya intloko ebuhlungu, umkhuhlane, ubuthathaka be-lymph node, kanciopenia kwaye njl njl. Kwiminyaka yakutshanje, ukutshintsha kwemozulu, ukufudukela kwemozulu, ukuphuculwa okukhawulezileyo kokhenketho kunye nezinye izinto zibonelele ngeemeko ezikhawulezayo nezilula zokuhambisa kunye nokusasazeka kwe-DF, ekhokelela ekukhuleni kwendawo ye-DF.
Ijelo
| Indlala | I-denv nucleid acid |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | -18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo locaphu | I-serium entsha |
| Ct | ≤38 |
| CV | <5% |
| Indawo yokuhlala | Iikopi ezingama-500 / ml |
| Ubuchule | Iziphumo zovavanyo lokuphazamiseka zibonisa ukuba xa uxinzelelo lweBilirubin kwi-168.2μ0lmol / ml, i-hemoglobin iveliswe yi-Hemolysis ayingaphezulu kwe-130g / L, i-igid yegazi ayingaphezulu kwe-65mmol / ml, i-Igg ipheleleyo Ugxininiso kwi-serum alikho ngaphezulu kwe-5mg / ml, akukho mpembelelo kwintsholongwane yeDengue, intsholongwane okanye I-viungoun virus I-Hepatitisi Intsholongwane, i-Hepatitis B intsholongwane, i-Hepatitis C inyulu, intsholongwane ye-Herpes, i-Hinavirus, i-virus yase-Intshona, i-Bunavirus, i-Bunavicus, iziphumo zibonisa ukuba akukho Impendulo enqamlezileyo phakathi kwale kit kunye nee pathons ezichazwe apha ngasentla. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystslates 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR Ukukhanya®I-480 yeyokwenene ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
I-Tiamp VIUS DNA / I-RNA Kit Ivolumu yesampulu ekhutshiweyo yi-140μl, ivolumu yesiqhelo ye-elt yi-60μl.
Inketho 2.
I-Macro kunye ne-RNA yeDNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-7-56), I-HWTS-3017-96) (HWTS-3006C, HWTS-3006B) I-LTD Ivolumu yesampulu ekhutshiwe yi-2007, kwaye ivolumu yesiqhelo ye-LELL i-80μl.
