Intsholongwane yeDengue, intsholongwane yeZika kunye neChikungunya Virus Multiplex
Igama lemveliso
I-HWTS-FE040 Dengue Virus, Zika Virus kunye neChikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umkhuhlane weDengue (DF), obangelwa lusulelo lwe-dengue virus (DENV), sesinye sezifo ezisulelayo ze-arbovirus ezisasazeka kakhulu. Indlela yokudlulisela i-Aedes aegypti kunye ne-Aedes albopictus. I-DF ixhaphake kakhulu kwiindawo ezishushu nezishushu. I-DENV yeye-flavivirus phantsi kwe-flaviviridae, kwaye inokwahlulwa ibe zii-serotypes ezi-4 ngokwe-antigen yomphezulu. Iimpawu zonyango zosulelo lwe-DENV ziquka intloko ebuhlungu, umkhuhlane, ubuthathaka, ukwanda kwe-lymph node, i-leukopenia njl.njl., kunye nokopha, ukothuka, ukwenzakala kwesibindi okanye nokufa kwiimeko ezinzima. Kwiminyaka yakutshanje, utshintsho lwemozulu, ukufudukela kwabantu ezidolophini, uphuhliso olukhawulezileyo lokhenketho kunye nezinye izinto zibonelele ngeemeko ezikhawulezayo nezilula zokusasaza nokusasazeka kwe-DF, nto leyo ekhokelela ekwandeni rhoqo kwendawo yobhubhane ye-DF.
Itshaneli
| I-FAM | I-DENV nucleic acid |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-serum entsha |
| Ct | ≤38 |
| CV | ＜5% |
| I-LoD | Iikopi ezingama-500/mL |
| Ukuchaneka | Iziphumo zovavanyo lokuphazamisa zibonisa ukuba xa uxinzelelo lwe-bilirubin kwi-serum lungekho ngaphezulu kwe-168.2μmol/ml, uxinzelelo lwe-hemoglobin oluveliswa yi-hemolysis alukho ngaphezu kwe-130g/L, uxinzelelo lwe-lipid egazini alukho ngaphezu kwe-65mmol/ml, uxinzelelo lwe-IgG iyonke kwi-serum alukho ngaphezu kwe-5mg/mL, akukho mpembelelo kwi-dengue virus, i-Zika virus okanye i-chikungunya virus. I-Hepatitis A virus, i-Hepatitis B virus, i-Hepatitis C virus, i-Herpes virus, i-Eastern equine encephalitis virus, i-Hantavirus, i-Bunya virus, i-West Nile virus kunye neesampuli ze-human genomic serum zikhethwa kuvavanyo lwe-cross-reactivity, kwaye iziphumo zibonisa ukuba akukho mpikiswano phakathi kwale kit kunye ne-pathogens ekhankanyiweyo apha ngasentla. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), kwaye ukukhupha kufuneka kwenziwe ngokuhambelana ngqo nemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-140μL, kwaye umthamo wokucoca ocetyiswayo yi-60μL.
Ukhetho 2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., kwaye ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
