Intsholongwane yeDengue, iZika Virus kunye neChikungunya Virus Multiplex
Igama lemveliso
HWTS-FE040 Virus Dengue, Zika Virus and Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Dengue fever (DF), ebangelwa yintsholongwane yedengue (DENV) usulelo, sesinye sezifo ezisulelayo ze-arbovirus.Uthungelwano lwayo lubandakanya i-Aedes aegypti kunye ne-Aedes albopictus.I-DF ixhaphake kakhulu kwimimandla yetropikhi kunye neyetropikhi.I-DENV yeye-flavivirus phantsi kwe-flaviviridae, kwaye ingahlelwa kwii-serotypes ze-4 ngokwe-antigen yomhlaba.Ukubonakaliswa kweklinikhi yosulelo lwe-DENV ikakhulu kubandakanya intloko ebuhlungu, umkhuhlane, ubuthathaka, ukwanda kwe-lymph node, i-leukopenia kunye nokunye, kunye nokopha, ukothuka, ukwenzakala kwesibindi okanye ukufa kwiimeko ezinzima.Kwiminyaka yakutshanje, utshintsho lwemozulu, ukufudukela kwabantu ezidolophini, uphuhliso olukhawulezayo lokhenketho kunye nezinye izinto zibonelele ngeemeko ezikhawulezayo neziluncedo zokusasaza kunye nokusasazwa kwe-DF, ekhokelela ekwandeni okuthe gqolo kobhubhane we-DF.
Umjelo
| FAM | MP nucleic acid |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Iserum entsha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lophazamiseko zibonisa ukuba xa uxinaniso lwebilirubin kwi-serum lungekho ngaphezu kwe-168.2μmol/ml, ugxininiso lwe-hemoglobin oluveliswa yi-hemolysis alukho ngaphezu kwe-130g/L, ukuxinwa kwe-lipid yegazi akukho ngaphezu kwe-65mmol/ml, i-IgG iyonke. Ukugxininiswa kwi-serum akukho ngaphezu kwe-5mg / mL, akukho mpembelelo kwintsholongwane ye-dengue, i-virus ye-Zika okanye i-chikungunya i-virus yokufunyanwa.Intsholongwane yeHepatitis A, intsholongwane yeHepatitis B, intsholongwane yeHepatitis C, intsholongwane yeHerpes, intsholongwane yeEquine encephalitis yaseMpuma, iHantavirus, intsholongwane yeBunya, intsholongwane yeWest Nile kunye neesampulu zeserum yabantu zikhethwa kuvavanyo lwe-cross-reactivity, kwaye iziphumo zibonisa ukuba akukho ukusabela okuphambeneyo phakathi kwale khithi kunye neentsholongwane ezichazwe ngasentla. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-TIANamp Virus DNA/RNA Kit (YDP315-R), kwaye ukukhutshwa kufuneka kuqhutywe ngokuhambelana ngqongqo nomyalelo wokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-140μL, kwaye umthamo we-lution ocetyiswayo yi-60μL.
Ukhetho lwesi-2.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd., kwaye ukukhutshwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
