Iintlobo ezisibhozo zeentsholongwane zokuphefumla
Igama lemveliso
I-HWTS-RT184-Iintlobo ezisibhozo zeentsholongwane zokuphefumla iNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Usulelo lwendlela yokuphefumla lolona hlobo luqhelekileyo lwesifo ebantwini, olunokwenzeka nakweyiphi na isini, ubudala kunye nommandla, kwaye sesinye sezona zizathu zibalulekileyo zokugula nokufa emhlabeni.[1]Iintsholongwane zokuphefumla eziqhelekileyo kwiklinikhi ziquka intsholongwane ye-influenza A (IFV A), intsholongwane ye-influenza B (IFV B), intsholongwane ye-respiratory syncytial, i-adenovirus, i-human metapneumovirus, i-rhinovirus, intsholongwane ye-parainfluenza (I/II/III) kunye ne-mycoplasma pneumoniae, njl.njl.[2,3]Iimpawu zeklinikhi kunye neempawu ezibangelwa lusulelo lwendlela yokuphefumla ziyafana, kodwa usulelo olubangelwa zizifo ezahlukeneyo luneendlela ezahlukeneyo zonyango, iziphumo zonyango kunye nendlela esiqala ngayo isifo.[4,5]Okwangoku, iindlela eziphambili zokuchonga iintsholongwane zokuphefumla elabhoratri ziquka: ukwahlulwa kwentsholongwane, ukufunyanwa kwe-antigen kunye nokufunyanwa kwe-nucleic acid. Le khithi ifumanisa kwaye ichonge ii-nucleic acids ezithile zentsholongwane kubantu abaneempawu zosulelo lokuphefumla, kunye nezinye iziphumo zeklinikhi nezelabhoratri ukunceda ekuchongweni kosulelo lwentsholongwane lokuphefumla.
Iiparameters zobugcisa
| Indawo yokugcina izinto | 2-8℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-200/mL |
| Ukuchaneka | Ukusabela okunqamlezileyo: Akukho kusabela okunqamlezileyo phakathi kwekhithi kunye nentsholongwane yeBoca, iCytomegalovirus, intsholongwane ye-Epstein-Barr, intsholongwane yeHerpes simplex, intsholongwane ye-varicella zoster, intsholongwane yeMumps, i-Enterovirus, intsholongwane yemasisi, intsholongwane ye-human coronavirus, iSARS Coronavirus, iMERS Coronavirus, iRotavirus, iNorovirus, iChlamydia pneumoniae, iStreptococcus pneumoniae, iKlebsiella pneumoniae, iStreptococcus pyogenes, iLegionella, iPneumospora, iHaemophilus influenzae, iBacillus pertussis, iStaphylococcus aureus, iMycobacterium tuberculosis, igonococcus, iCandida albicans, iCandida glabra, iAspergillus fumigatus, iCryptococcus neoformans, iStreptococcus salivarius, iMoraxella catarrh, iLactobacillus, iCorynebacterium, iDNA ye-human genomic. Uvavanyo lokuphazamisa: Khetha i-mucin (60mg/mL), igazi lomntu (50%), i-benephrin (2mg/mL), i-hydroxymethazoline (2mg/mL) 2mg/mL), i-sodium chloride ene-5% yesigcini (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-fluniacetone (20μg/mL), i-triamcinolone (2mg/mL), i-budesonide (1mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-benzocaine (10%), i-menthol (10%), i-zanamivir (20mg/mL), i-peramivir (1mg/mL), i-mupirocin (20mg/mL), i-tobramycin (0.6mg/mL), i-oseltamivir (60ng/mL), i-ribavirin (10mg/L), Iziphumo zibonise ukuba izinto eziphazamisayo kwi-concentra engentla |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Ii-Applied Biosystems 7500 Real-Time PCR Systems, iQuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela, iiNkqubo ze-SLAN-96P zeXesha langempela ze-PCR (iHongshi Medical Technology Co., Ltd.), iiNkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 yeXesha langempela ye-Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), iNkqubo ye-PCR yeXesha langempela ye-BioRad CFX96, iNkqubo ye-PCR yeXesha langempela ye-BioRad CFX Opus 96. Isebenza kwi-type II detection reagent: EudemonTM AIO800 (HWTS-EQ007) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
