Iintlobo ezisibhozo zeeNtsholongwane zokuphefumla
Igama lemveliso
HWTS-RT184-Iindidi ezisibhozo zeeNxulumana zokuphefumla zeNucleic Acid yeKit (Fluorescence PCR)
I-Epidemiology
Usulelo lwendlela yokuphefumla lolona hlobo luxhaphakileyo lwesifo somntu, esinokuthi senzeke nakwesiphi na isini, ubudala kunye nommandla, kwaye sesinye sezona zizathu zibalulekileyo zokugula nokufa emhlabeni.[1]. Iintsholongwane zeklinikhi eziqhelekileyo zokuphefumla ziquka intsholongwane yomkhuhlane A (IFV A), intsholongwane ye-influenza B (IFV B), intsholongwane ye-syncytial yokuphefumla, i-adenovirus, i-metapneumovirus yabantu, i-rhinovirus, i-parainfluenza virus (I / II / III) kunye ne-mycoplasma pneumoniae, njl.[2,3]. Iimpawu zeklinikhi kunye neempawu ezibangelwa usulelo lwendlela yokuphefumla ziyafana, kodwa usulelo olubangelwa zizifo ezahlukeneyo zineendlela ezahlukeneyo zonyango, iziphumo zonyango kunye nekhosi yesifo.[4,5]. Okwangoku, iindlela eziphambili zokufumanisa i-laboratory ye-pathogens yokuphefumula zibandakanya: ukuhlukaniswa kwentsholongwane, ukufumanisa i-antigen kunye nokufumanisa i-nucleic acid. Le khithi ibona kwaye ichonge i-nucleic acids yentsholongwane ethile kubantu abaneempawu kunye neempawu zosulelo lokuphefumla, ngokubambisana nezinye iziphumo zeklinikhi kunye nezaselebhu zokuncedisa ekuxilongeni usulelo lwentsholongwane yokuphefumula.
Iiparamitha zobuGcisa
| Ugcino | 2-8℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | i-oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Iikopi ezingama-200/mL |
| Ukuchaza ngokuthe ngqo | Ukuphinda usebenze kwakhona: Akukho kuphinda kusebenze phakathi kwekiti kunye nentsholongwane ye-Boca, i-Cytomegalovirus, i-Epstein-Barr virus, i-Herpes simplex virus, i-varicella zoster Virus, i-Mumps virus, i-Enterovirus, intsholongwane yemasisi, i-coronavirus yabantu, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-Streptococ pneumoniae, i-Kreptococcus Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, iStreptococcus calicoccus, iLalictococcus I-Corynebacterium, i-DNA ye-genomic yomntu. Uvavanyo lokuphazamiseka: Khetha i-mucin (60mg / mL), igazi lomntu (50%), i-benephrin (2mg / mL), i-hydroxymethazoline (2mg / mL) 2mg / mL), i-sodium chloride ene-5% yokukhusela (20mg / mL), i-beclomethasone (20mg / mL), i-dexamethasone / l0mg / L0mL), i-dexamethasone / l0mg (20mg) triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Iziphumo zibonise ukuba izinto eziphazamisayo kule concentra ingentla |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: I-Biosystems esetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®IiNkqubo ze-PCR ezi-5 zexesha langempela, i-SLAN-96P ye-Real-Time PCR Systems (i-Hongshi Medical Technology Co., Ltd.), i-LineGene 9600 Plus Iinkqubo ze-PCR zokufumanisa iXesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 ye-Real-Time Quantitative Thermal Cycler Co. Inkqubo, i-BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: I-Eudemon TM AIO800 (HWTS-EQ007) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
