I-Enterovirus 71 (ev71)
Igama lemveliso
I-HWTS -V003- I-Entetrovirus 71
Isipikumu
Isifo somlomo we-Hander sisifo esosulelayo esibangelwa yi-Enterovirus (EV). Okwangoku, iindidi ezili-108 ze-serobirpes ze-enterovirus ziye zafunyanwa, ezahlulahlulwe zangamaqela amane: A, B, i-Entetrovirus EV716 zii-pathogns eziphambili. Esi sifo senzeka ikakhulu kubantwana abangaphantsi kweminyaka emi-5, kwaye sinokubangela i-hespes ezandleni, iinyawo, umlomo kunye namanye amalungu. Inani elincinci labantwana liya kuhlakulela iingxaki ezinje nge-Myocarditis, i-pulmonary yede, kunye ne-Aseptic Meningphatis.
Ijelo
| Indlala | Ev71 |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo locaphu | I-Orophaorsenge in swab,I-herpes ilwela |
| Ct | ≤35 |
| CV | <5.0% |
| Indawo yokuhlala | I-500COPIes / ml |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR Ukukhanya®I-480 ye-PCR ye-PCR ye-PCR I-Tlinegene 9600 kunye ne-Pood-Time-Dead-Times Proction Spice Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
Ukucetyiswa ukwenziwa kwe-Reagent: I-Macro kunye neMacro kunye neMicro-Micro Pagent (HWTS-30055-8), kwaye i-Shoption kufuneka iqhutywa ngokomyalelo wokusetyenziswa. Iisampulu ezikhutshiweyo ziyi-oropharyngezwaal swibs okanye iisampulu ze-herpes ezivela kwizigulana eziqokelelwe kwisiza. Yongeza i-Swabs eqokelelweyo kwi-Macro kunye neMicroCaration Inguqulelo yokuqaliswa kwe-Macro kunye ne-HWTS-3005-8) ngokuthe ngqo, i-vortex kwaye idibanise igumbi lempahla kangangemizuzu emi-5, ithathe i-RNA Kwisampulu nganye.
Inketho 2.
Ukucetyiswa Ukuqulunqwa kwe-Reagent: I-MACR & I-MACRE & HWA K Kit (HWTS-3017-50, HWTS-3017-36, HWTS-3017-56) Isikhupheli se-nucleic esizenzekelayo (i-HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & I-Micro-Test Count-Tex Count-Te., kwaye i-LTD. Ivolumu yesampulu ekhutshiwe yi-2007, kwaye ivolumu yesiqhelo ye-LELL i-80μl.
Inketho3.
Ukucetyiswa ukwenziwa kwakhona: I-Qiaamp Vial RNA Mini Kit (i-52904) yi-Qiagen okanye i-Tiamp virus DNA / RNA K Kit Ivolumu yesampulu ekhutshiweyo yi-140μl, ivolumu yesiqhelo ye-elt yi-60μl.
