I-Enterovirus 71 (EV71)
Igama lemveliso
Ikhithi yokuFumanisa i-Nucleic Acid ye-HWTS-EV003- Enterovirus 71 (EV71) (i-Fluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla sisifo esosulelayo esibangelwa zii-enteroviruses (EV). Okwangoku, kufunyenwe iintlobo ezili-108 zee-serotypes zee-enteroviruses, ezahlulwe zangamaqela amane: A, B, C kunye no-D. Phakathi kwazo, i-enterovirus EV71 kunye ne-CoxA16 zezona zifo ziphambili. Esi sifo sixhaphake kakhulu kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye sinokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo. Inani elincinci labantwana liya kuba neengxaki ezifana ne-myocarditis, i-pulmonary edema, kunye ne-aseptic meningoencephalitis.
Itshaneli
| I-FAM | EV71 |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab,Ulwelo lweHerpes |
| Ct | ≤35 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Iinkqubo ze-PCR zeXesha langempela ezingama-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), kwaye ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Iisampuli ezikhutshiweyo zii-oropharyngeal swabs okanye iisampuli ze-herpes fluid ezivela kwizigulana eziqokelelwe kwindawo. Yongeza ii-swabs eziqokelelweyo kwi-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ngqo, udibanise kakuhle, ubeke kubushushu begumbi imizuzu emi-5, uzikhuphe uze uziguqule ukuze zixutywe kakuhle, ukuze ufumane i-RNA yesampuli nganye.
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., kwaye ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-200µL, kwaye umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 3.
I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN okanye i-TIANamp Virus DNA/RNA Kit (YDP315-R), kwaye ukukhupha kufuneka kwenziwe ngokuhambelana ngqo nemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-140μL, kwaye umthamo wokucoca ocetyiswayo yi-60μL.
