I-Enterovirus 71 (EV71)
Igama lemveliso
I-HWTS-EV003- Enterovirus 71 (EV71) iNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hand-foot-mouth isifo sisifo esosulelayo esibangelwa yi-enteroviruses (EV).Okwangoku, iintlobo ze-108 ze-serotypes ze-enteroviruses zifunyenwe, ezihlulwe zibe ngamaqela amane: A, B, C kunye no-D. Phakathi kwazo, i-enterovirus EV71 kunye ne-CoxA16 ziyi-pathogens eziphambili.Esi sifo sivela kakhulu kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye kunokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo.Inani elincinane labantwana liya kuba neengxaki ezifana ne-myocarditis, i-pulmonary edema, kunye ne-aseptic meningoencephalitis.
Umjelo
| FAM | EV71 |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab,Ulwelo lweHerpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Iikopi/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®IiNkqubo ze-PCR ze-480 zeXesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), kwaye ukukhutshwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa.Iisampuli ezikhutshiweyo zi-oropharyngeal swabs okanye iisampulu ze-herpes fluid ezivela kwizigulane eziqokelelwe kwindawo.Yongeza i-swabs eqokelelweyo kwi-Macro kunye ne-Micro-Test Release Release Reagent (HWTS-3005-8) ngokuthe ngqo, i-vortex kwaye udibanise kakuhle, ubeke kwindawo yokushisa imizuzu emi-5, uzikhuphe kwaye emva koko uguqule ukuze udibanise kakuhle, ukufumana i-RNA. yesampulu nganye.
Ukhetho lwesi-2.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd., kwaye ukukhutshwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-200µL, kwaye umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho3.
Ukukhutshwa kwe-reagent ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) yi-QIAGEN okanye i-TIANamp Virus DNA/RNA Kit (YDP315-R), kwaye ukukhutshwa kufuneka kuqhutywe ngokuhambelana ngqongqo nomyalelo wokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-140μL, kwaye umthamo we-elution ocetyiswayo yi-60µL.
