HCV Genotyping

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-genotyping ye-virus ye-hepatitis C (i-HCV) i-subtypes ye-1b, i-2a, i-3a, i-3b kunye ne-6a kwi-serum yeklinikhi / iisampuli ze-plasma ze-virus ye-hepatitis C (HCV).Inceda ekuxilongeni nasekunyangweni kwezigulane ze-HCV.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-HP004-HCV Genotyping Kit yokuFumana (Fluorescence PCR)

I-Epidemiology

Intsholongwane ye-Hepatitis C (HCV) yeyosapho lwe-flaviviridae, kwaye i-genome yayo yi-RNA enye echanekileyo, eguqulwa ngokulula.Intsholongwane ikhona kwi-hepatocytes, i-serum leukocytes kunye ne-plasma yabantu abosulelekileyo.Ufuzo lwe-HCV luchaphazeleka ekuguqukeni kwaye lunokwahlulwa ubuncinane kwii-genotypes ezi-6 kunye nee-subtypes ezininzi.I-HCV genotypes ezahlukeneyo zisebenzisa iirejimeni zonyango ze-DAA ezahlukeneyo kunye nezifundo zonyango.Ngoko ke, phambi kokuba izigulana zinyangwe ngonyango lwe-DAA lwe-antiviral, i-HCV genotype kufuneka ibonwe, kwaye nakwizigulana ezinohlobo loku-1, kuyimfuneko ukwahlula ukuba luhlobo 1a okanye uhlobo 1b.

Umjelo

FAM Uhlobo 1b, Uhlobo 2a
ROX Uhlobo 6a, Uhlobo lwe-3a
VIC/HEX Ulawulo lwangaphakathi, Uhlobo lwe-3b

IiParameters zobuGcisa

Ugcino ≤-18℃ ebumnyameni
Beka ubomi kwishelufa Iinyanga ezili-9
Uhlobo loMfanekiso ISerum, iPlasma
Ct ≤36
CV ≤5.0%
LoD 200 IU/mL
Ukuchaza ngokuthe ngqo Sebenzisa le khithi ukubona ezinye iintsholongwane okanye iisampuli zebhaktheriya ezifana: i-cytomegalovirus yabantu, i-Epstein-Barr virus, i-virus ye-human immunodeficiency, i-virus ye-hepatitis B, i-virus ye-hepatitis A, i-syphilis, i-herpes virus yohlobo lwe-6, i-herpes virus ye-herpes 1, i-herpes virus uhlobo lwe-2, intsholongwane ye-influenza A, i-Propionibacterium acnes, i-Staphylococcus aureus, i-Candida albicans, njl. Iziphumo zonke zibi.
Izixhobo Ezisebenzayo Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike.
Iinkqubo zePCR ze-ABI 7500
ABI 7500 Fast Real-Time PCR Systems
SLAN-96P Iinkqubo zePCR zeXesha langempela
I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela
Iinkqubo zePCR zeLightCycler®480
LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems
MA-6000 Real-Time Quantitative Thermal Cycler
I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani
BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

hcv


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