Intsholongwane ye-Forest Encephalitis
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-FE006 ye-Forest Encephalitis Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Forest encephalitis (FE), eyaziwa ngokuba yi-tick-borne encephalitis (i-tick-borne encephalitis, TBE), sisifo esosulelayo esihlasela inkqubo yemithambo-luvo esibangelwa yi-forest encephalitis virus. I-Forest encephalitis virus ikwi-genus Flavivirus yosapho lweFlaviviridae. Amasuntswana e-virus angqukuva anobubanzi obuyi-40-50nm. Ubunzima be-molecular bumalunga ne-4×106I-Da, kwaye i-genome yentsholongwane yi-RNA ene-positive-sense, ene-single-strand.[1]Ngokwezonyango, ibonakaliswa ngumkhuhlane ophezulu, intloko ebuhlungu, i-coma, ukuqala ngokukhawuleza kokucaphuka kwe-meningeal, kunye nokukhubazeka kwemisipha yentamo kunye namalungu, kwaye inezinga eliphezulu lokufa. Ukuxilongwa kwangoko nangokukhawuleza kwentsholongwane ye-encephalitis yehlathi ngundoqo kunyango lwe-encephalitis yehlathi, kwaye ukusekwa kwendlela elula, ecacileyo nekhawulezayo yokuxilonga i-etiological kubaluleke kakhulu ekuxilongweni kwe-encephalitis yehlathi.[1,2].
Itshaneli
| I-FAM | i-nucleic acid yentsholongwane ye-encephalitis yehlathi |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | iserum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R) yiTiangen Biotech(Beijing) Co.,Ltd., ukukhupha kufuneka kwenziwe ngokwemiyalelo engqongqo. Umthamo wesampulu okhutshiweyo ocetyiswayo yi-140μL kwaye umthamo wokucoca ocetyiswayo yi-60μL.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Ukukhupha kufuneka kwenziwe ngokwemiyalelo engqongqo. Umthamo wesampulu ocetyiswayo yi-200μL kwaye umthamo wokucoca ocetyiswayo yi-80μL.
