Intsholongwane yehlathi ihlathi
Igama lemveliso
I-HWTS-FO006 ihlathi i-chineffalis virus inycleid ikhithi ye-Acid iKit (Fluorescence PCR)
Isipikumu
Ihlathi lencephalis (Fe), ekwabizwa ngokuba yi-Tipne Encephalis (TREP-BICEPOAlis, TBE), sisifo esosulelayo se-ofisi ye-nencephalis ephambili esibangelwa yintsholongwane ye-encephalis ephakathi. Intsholongwane yeHlathi Encephalis intsholongwane ye-Flavivirus ye-Flavividi Davididae yosapho. Amasuntswana amantshontsho aneziseko ezinobubanzi obuyi-40-50mm. Ubunzima bemolekyuli buyi-4 × 106Da, kwaye intsholongwane i-genome yimvakalelo elungileyo, i-rna enye[1]. Ngokwesinyane, ibonakaliswa ngumkhuhlane omkhulu, intloko ebuhlungu, i-coma, ukuqala kwengcungcutheka ye-zungual, kunye nokukhubazeka kwemisipha yentamo kunye nenqanaba lemilenze, kwaye linenqanaba lokufa. Ukuqala kwesifo sehlathi yehlathi yehlathi sisitshixo sokunyanga i-Encephalis, kunye nokusekwa kwendlela elula, eyodwa nekhawulezayo kunye nekhawulezayo nekhawulezayo yokufihla ngokukhawuleza kubaluleke kakhulu[I-1,2].
Ijelo
Indlala | ihlathi le-encephalis virus nucleic acid |
Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
Ukugcinwa | ≤-18 ℃ |
Beka ubomi kwishelufa | Iinyanga ezili-9 |
Uhlobo locaphu | I-serium entsha |
Tt | ≤38 |
CV | ≤5.0% |
Indawo yokuhlala | I-500COPIes / ml |
Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR Ukukhanya®I-480 yeyokwenene ye-PCR I-Tlinegene 9600 kunye ne-Pood-Time-Dead-Times Proction Spice Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Ukucetyiswa ukwenziwa kwe-Reagent: Ukukhutshwa kwe-nucleic acid (Ikhithi yokucoca (I-YDP35-R) nguTiangen Biotech (Beijing) Co., i-LTD., I-LTD., I-LTD., I-LTD., I-LTD. Ivolumu yesampulu ekhuthazwe yi-140μl kwaye ivolumu yesiqhelo ye-elt yi-60μl.
Ukucetyiswa Ukuqala I-HWTS-3006, HWTS-3006C, HWTS-3006B). Ukukhutshwa kufuneka kuqhutywa ngokwemiyalelo ngokungqongqo. Ivolumu yesampulu ekhuthazwe yi-200μ kunye nevolumu yesiqhelo ye-Elpress yi-80μl.