I-Forest Encephalitis Virus
Igama lemveliso
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Forest encephalitis (FE), ekwaziwa ngokuba yi-incephalitis yekhalane (I-Tick-borne encephalitis, i-TBE), sisifo esosulelayo esiqatha kwinkqubo yemithambo-luvo esibangelwa yintsholongwane ye-encephalitis yamahlathi. Intsholongwane ye-encephalitis yehlathi yeyohlobo lweFlavivirus yosapho lweFlaviviridae. Iintsholongwane zentsholongwane zingqukuva kunye nobubanzi obuyi-40-50nm. Ubunzima be-molecular malunga ne-4 × 106Da, kunye ne-virus genome yi-positive-sense, i-RNA enomsonto omnye[1]. Ngokonyango, kubonakala ngumkhuhlane ophezulu, intloko ebuhlungu, i-coma, ukuqala ngokukhawuleza kwe-meningeal irritation, kunye nokukhubazeka kwemisipha yentamo kunye nemilenze, kwaye inezinga eliphezulu lokufa. Ukuxilongwa kwangoko kunye nokukhawuleza kwentsholongwane ye-encephalitis yehlathi ngundoqo kunyango lwe-encephalitis yamahlathi, kwaye ukusekwa kwendlela elula, ecacileyo kunye nekhawulezayo yokuxilongwa kwe-etiological ibaluleke kakhulu ekuxilongweni kweklinikhi ye-encephalitis yamahlathi.[1,2].
Umjelo
| FAM | ihlathi encephalitis virus nucleic acid |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Iikopi/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd., ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo ngokungqongqo. Umthamo wesampulu ephakanyisiweyo ecetyiswayo yi-140μL kwaye umthamo we-elution ocetyiswayo yi-60μL.
I-reagent yokutsalwa ecetyiswayo: IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (H3006060-HWTS-30, HWTS-3017-96) HWTS-3006B). ukutsalwa kufuneka kuqhutywe ngokwemiyalelo ngokungqongqo. Umthamo wesampulu ecetyiswayo ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-80μL.
