Iintlobo ezili-11 zeentsholongwane zokuphefumla ezomileyo ngokuqandisa i-Nucleic Acid
Igama lemveliso
I-HWTS-RT190 -Yomiswe ngokuqandisa-yomiswe ngokuqandisa Iintlobo ezili-11 ze-Respiratory Pathogens Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Usulelo lwendlela yokuphefumla sisifo esibalulekileyo esisongela impilo yoluntu kakhulu. Izifundo zibonise ukuba uninzi lwezifo zendlela yokuphefumla zibangelwa ziintsholongwane kunye/okanye iintsholongwane ezibangela ukuba umntu asuleleke, nto leyo ekhokelela ekwandeni kobunzima besifo okanye nokufa. Ke ngoko, ukuchonga intsholongwane kunokubonelela ngonyango olujoliswe kulo kwaye kuphucule izinga lokusinda kwesigulana[1,2]. Nangona kunjalo, iindlela zemveli zokufumanisa izifo zendlela yokuphefumla ziquka uvavanyo lwe-microscopic, inkcubeko yeentsholongwane, kunye novavanyo lwe-immunological. Ezi ndlela ziyinkimbinkimbi, zithatha ixesha, zifuna ubuchwepheshe, kwaye zinobuthathaka obuphantsi. Ukongeza, azinakubona izifo ezininzi kwisampulu enye, okwenza kube nzima ukubonelela oogqirha ngoxilongo oluchanekileyo. Ngenxa yoko, uninzi lwamayeza lusekwinqanaba lonyango olusebenzayo, olungakhawulezi nje kuphela umjikelo wokuxhathisa iintsholongwane, kodwa lukwachaphazela ukuxilongwa kwezigulane ngexesha elifanelekileyo[3]. I-Common Haemophilus influenzae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Klebsiella pneumoniae, i-Streptococcus pneumoniae, i-Stenotrophomonas maltophilia, i-Bordetella pertussis, i-Bordetella parapertussis, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, kunye ne-Legionella pneumophila zizinto ezibalulekileyo ezibangela usulelo lwe-nosocomial respiratory tract [4,5]. Le khithi yovavanyo ifumanisa kwaye ichonge ii-nucleic acids ezithile zezifo ezingasentla kubantu abaneempawu kunye neempawu zosulelo lokuphefumla, kwaye iyidibanise nezinye iziphumo zelebhu ukunceda ekuchongweni kosulelo lwe-pathogen yokuphefumla.
Iiparameters zobugcisa
| Indawo yokugcina izinto | 2-30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomphimbo |
| Ct | ≤33 |
| CV | <5.0% |
| I-LoD | I-LoD yekhithi yeKlebsiella pneumoniae yi-500 CFU/mL; i-LoD yeStreptococcus pneumoniae yi-500 CFU/mL; i-LoD yeHaemophilus influenzae yi-1000 CFU/mL; i-LoD yePseudomonas aeruginosa yi-500 CFU/mL; i-LoD ye-Acinetobacter baumannii yi-500 CFU/mL; i-LoD yeStenotrophomonas maltophilia yi-1000 CFU/mL; i-LoD yeBordetella pertussis yi-500 CFU/mL; i-LoD yeBordetella parapertussis yi-500 CFU/mL; i-LoD yeMycoplasma pneumoniae yi-200 copies/mL; i-LoD yeLegionella pneumophila yi-1000 CFU/mL; I-LoD yeChlamydia pneumoniae yiikopi ezingama-200/mL. |
| Ukuchaneka | Akukho mpendulo idibeneyo phakathi kwekhithi kunye nezinye izifo ezixhaphakileyo zokuphefumla ngaphandle koluhlu lokufumanisa lwekhithi yovavanyo, umz. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, kunye neCandida tropicalis. |
| Izixhobo Ezisebenzayo | Uhlobo I: Iinkqubo ze-PCR eziSebenzisiweyo 7500 zeXesha langempela, iiNkqubo zePCR eziSebenzisiweyo 7500 zeXesha langempela, iQuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela, iiNkqubo ze-SLAN-96P zeXesha langempela ze-PCR (iHongshi Medical Technology Co., Ltd.), iLightCycler®Iinkqubo ze-PCR zeXesha Eliqhelekileyo ezingama-480, iiNkqubo zokuFumanisa i-PCR zeXesha Eliqhelekileyo zeLineGene 9600 Plus (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), iNkqubo ye-PCR yeXesha Eliqhelekileyo yeBioRad CFX96, iNkqubo ye-PCR yeXesha Eliqhelekileyo yeBioRad CFX Opus 96. Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co. Ltd. |
Ukuhamba komsebenzi
Uhlobo I: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ziyacetyiswa xa kutsalwa isampuli kwaye amanyathelo alandelayo kufuneka aqhutywe ngokuhambelana ne-IFU yeKit.
