Umkhenkce-omisiweyo Iindidi ezili-11 zeePathogens zokuphefumla iNucleic Acid
Igama lemveliso
HWTS-RT190 -Umkhenkce-umkhenkce-womileyo Iindidi ezili-11 zePathogens eziphefumlayo zeNucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Usulelo lomgudu wokuphefumla sisifo esibalulekileyo esisongela kakhulu impilo yoluntu. Uphononongo lubonise ukuba uninzi losulelo lwendlela yokuphefumla lubangelwa ziintsholongwane zebhaktheriya kunye/okanye zentsholongwane eyosulela umkhosi, nto leyo ekhokelela ekunyukeni kobunzima besifo okanye nokufa. Ngoko ke, ukuchonga i-pathogen kunokubonelela ngonyango olujoliswe kuyo kunye nokuphucula izinga lokusinda kwesigulane [1,2]. Nangona kunjalo, iindlela zemveli zokufumanisa iintsholongwane zokuphefumla zibandakanya uvavanyo lwe-microscopic, inkcubeko yebhaktheriya, kunye novavanyo lwe-immunological. Ezi ndlela zintsonkothile, zidla ixesha, zifuna ubuchwephesha, kwaye zinovakalelo oluphantsi. Ukongeza, abanako ukubona iintsholongwane ezininzi kwisampulu enye, okwenza kube nzima ukubonelela oogqirha ngoxilongo oluchanekileyo. Ngenxa yoko, uninzi lwamayeza asekwinqanaba lamayeza anamandla, angakhawulezisi nje umjikelo woxhathiso lwebhaktiriya, kodwa achaphazela ukuxilongwa kwangethuba kwezigulana[3]. I-Common Haemophilus influenzae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Klebsiella pneumoniae, i-Streptococcus pneumoniae, i-Stenotrophomonas maltophilia, i-Bordetella pertussis, i-Bordetella parapertussis, i-Mycoplasma pneumoniae, i-Chlamydia pneumonia ebaluleke kakhulu, i-Chlamydia pneumonia ebaluleke kakhulu. usulelo lwe-nosocomial phecana lokuphefumula [4,5]. Le khithi yovavanyo ibona kwaye ichonge ii-nucleic acids ezithile zezi ntsholongwane zingasentla kubantu abaneempawu kunye neempawu zosulelo lokuphefumla, kwaye idibanisa kunye nezinye iziphumo zaselabhoratri ukuncedisa ekuxilongeni usulelo lwentsholongwane yokuphefumla.
Iiparamitha zobuGcisa
| Ugcino | 2-30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Ukuswabha komqala |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | I-LoD yekhithi ye-Klebsiella pneumoniae yi-500 CFU/mL; i-LoD ye-Streptococcus pneumoniae yi-500 CFU / mL; i-LoD ye-Haemophilus influenzae yi-1000 CFU/mL; i-LoD ye-Pseudomonas aeruginosa yi-500 CFU/mL; i-LoD ye-Acinetobacter baumannii yi-500 CFU/mL; i-LoD ye-Stenotrophomonas maltophilia yi-1000 CFU/mL; i-LoD yeBordetella pertussis yi-500 CFU / mL; i-LoD ye-Bordetella parapertussis yi-500 CFU / mL; i-LoD ye-Mycoplasma pneumoniae ziikopi ezingama-200/mL; i-LoD yeLegionella pneumophila yi-1000 CFU/mL; i-LoD yeChlamydia pneumoniae ngama-200 eekopi/mL. |
| Ukuchaza ngokuthe ngqo | Akukho mpendulo phakathi kwekiti kunye nezinye iipathogens eziqhelekileyo zokuphefumla ngaphandle koluhlu lokufumanisa ikiti yovavanyo, umz. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoccus, Micrococcus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influesperanza virus, Influesperanza virus virus Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, kunye neCandida tropicalis. |
| Izixhobo Ezisebenzayo | Uhlobo I: Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo ze-PCR zeXesha langempela, ii-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo, i-QuantStudio®IiNkqubo ze-PCR ze-5 zexesha langempela, i-SLAN-96P ye-Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), i-LightCycler®IiNkqubo ze-PCR ze-480 zeXesha langempela, i-LineGene 9600 Plus ye-Real-Time PCR Detection Systems (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 yeXesha langempela le-Cycler ye-Thermal Cycler (i-Suzhou Molarray Co., Ltd.), i-BioRad CFX96 ye-Real-Time PCR System, i-BioRad ye-PCR ye-Real-Time ye-PCR ye-PCR. Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co. Ltd. |
Ukuhamba komsebenzi
Uhlobo I: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co. ngokuhambelana ne-IFU yeKit.
