Hantaan Virus Nucleic
Igama lemveliso
HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hantavirus luhlobo lwentsholongwane ye-RNA egqunyiweyo, ecandekileyo, ene-negative-strand. IHantavirus yahlulahlulwe yaba ziindidi ezimbini: enye ibangela iHantavirus pulmonary syndrome (HPS), kwaye enye ibangela iHantavirus hemorrhagic fever ene-renal syndrome (HFRS). Olokuqala luxhaphake kakhulu eYurophu nase-United States, kwaye lo mva ngumkhuhlane we-hemorrhagic kunye nesifo sezintso esibangelwa yintsholongwane ye-Hantaan, exhaphakileyo eTshayina. Iimpawu ze-hantavirus uhlobo lwe-hantaan lubonakala ikakhulu njengomkhuhlane we-hemorrhagic kunye nesifo sezintso, esibonakaliswa ngumkhuhlane ophezulu, i-hypotension, ukopha, i-oliguria okanye i-polyuria kunye nokunye ukuphazamiseka komsebenzi wezintso. I-pathogenic kubantu kwaye kufuneka ihlawulwe ngokwaneleyo ingqalelo.
Umjelo
| FAM | uhlobo lwehantavirus hantaan |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampuli yokutsalwa yi-200μL. Umthamo we-elution ocetyiswayo yi-80μL.
Ii-reagents zokutsalwa ezicetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R). Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU. Umthamo wesampulu yokutsalwa yi-140μL. Umthamo we-elution ocetyiswayo yi-60μL.
