I-Hantaan Virus Nucleic
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-FE005 Hantaan Virus Nucleic Acid (iFluorescence PCR)
I-Epidemiology
IHantavirus luhlobo lwentsholongwane ye-RNA egutyungelweyo, ehlukaniswe, nenegative-strand. IHantavirus yahlulwe yaba ziintlobo ezimbini: enye ibangela iHantavirus pulmonary syndrome (HPS), kwaye enye ibangela iHantavirus hemorrhagic fever ene-renal syndrome (HFRS). Eyokuqala ixhaphake kakhulu eYurophu nase-United States, kwaye eyesibini yi-hemorrhagic fever ene-renal syndrome ebangelwa yiHantaan virus, exhaphakileyo eTshayina. Iimpawu zohlobo lwehantaan lwehantavirus zibonakala kakhulu njenge-hemorrhagic fever ene-renal syndrome, ebonakaliswa ngumkhuhlane ophezulu, uxinzelelo lwegazi, ukopha, i-oliguria okanye i-polyuria kunye nokunye ukuphazamiseka kokusebenza kwezintso. Iyingozi ebantwini kwaye kufuneka inikwe ingqalelo eyaneleyo.
Itshaneli
| I-FAM | uhlobo lwe-hantavirus hantaan |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | iserum entsha |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwe-IFU. Umthamo wesampulu yokukhupha yi-200μL. Umthamo wokucoca ocetyiswayo yi-80μL.
Ii-reagents zokukhupha ezicetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R). Ukukhupha kufuneka kwenziwe ngokwe-IFU. Umthamo wesampulu yokukhupha yi-140μL. Umthamo wokucoca ocetyiswayo yi-60μL.
